Widefield quantitative fluorescence imaging applied to tumor margin assessment

2021 ◽  
Author(s):  
Silvère Ségaud ◽  
Labrinus van Manen ◽  
Lysanne D.A. N. de Muynck ◽  
J. Sven D. Mieog ◽  
Alexander L. Vahrmeijer ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Margin Assessment ◽  
Tumor Margin ◽  
Quantitative Fluorescence

System and methods for wide-field quantitative fluorescence imaging during neurosurgery

2013 ◽  
Vol 38 (15) ◽  
pp. 2786 ◽  
Author(s):  
Pablo A. Valdes ◽  
Valerie L. Jacobs ◽  
Brian C. Wilson ◽  
Frederic Leblond ◽  
David W. Roberts ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Wide Field ◽  
Quantitative Fluorescence

Quantitative spectral reflectance imaging device for intraoperative breast tumor margin assessment

2009 ◽  
Author(s):  
N. Ramanujam ◽  
J.Q. Brown ◽  
T.M. Bydlon ◽  
S.A. Kennedy ◽  
L.M. Richards ◽  
...  
Keyword(s):  
Spectral Reflectance ◽  
Breast Tumor ◽  
Margin Assessment ◽  
Imaging Device ◽  
Tumor Margin

scholarly journals Quantitative fluorescence in intracranial tumor: implications for ALA-induced PpIX as an intraoperative biomarker

2011 ◽  
Vol 115 (1) ◽  
pp. 11-17 ◽  
Author(s):  
Pablo A. Valdés ◽  
Frederic Leblond ◽  
Anthony Kim ◽  
Brent T. Harris ◽  
Brian C. Wilson ◽  
...  
Keyword(s):  
Brain Tissue ◽  
Fluorescence Imaging ◽  
Low Grade ◽  
Normal Brain ◽  
Intracranial Tumors ◽  
Neoplastic Tissue ◽  
Fluorescence Measurements ◽  
Significant Difference ◽  
Quantitative Fluorescence

Object Accurate discrimination between tumor and normal tissue is crucial for optimal tumor resection. Qualitative fluorescence of protoporphyrin IX (PpIX), synthesized endogenously following δ-aminolevulinic acid (ALA) administration, has been used for this purpose in high-grade glioma (HGG). The authors show that diagnostically significant but visually imperceptible concentrations of PpIX can be quantitatively measured in vivo and used to discriminate normal from neoplastic brain tissue across a range of tumor histologies. Methods The authors studied 14 patients with diagnoses of low-grade glioma (LGG), HGG, meningioma, and metastasis under an institutional review board–approved protocol for fluorescence-guided resection. The primary aim of the study was to compare the diagnostic capabilities of a highly sensitive, spectrally resolved quantitative fluorescence approach to conventional fluorescence imaging for detection of neoplastic tissue in vivo. Results A significant difference in the quantitative measurements of PpIX concentration occurred in all tumor groups compared with normal brain tissue. Receiver operating characteristic (ROC) curve analysis of PpIX concentration as a diagnostic variable for detection of neoplastic tissue yielded a classification efficiency of 87% (AUC = 0.95, specificity = 92%, sensitivity = 84%) compared with 66% (AUC = 0.73, specificity = 100%, sensitivity = 47%) for conventional fluorescence imaging (p < 0.0001). More than 81% (57 of 70) of the quantitative fluorescence measurements that were below the threshold of the surgeon's visual perception were classified correctly in an analysis of all tumors. Conclusions These findings are clinically profound because they demonstrate that ALA-induced PpIX is a targeting biomarker for a variety of intracranial tumors beyond HGGs. This study is the first to measure quantitative ALA-induced PpIX concentrations in vivo, and the results have broad implications for guidance during resection of intracranial tumors.

scholarly journals Label-free reflectance hyperspectral imaging for tumor margin assessment: a pilot study on surgical specimens of cancer patients

2017 ◽  
Vol 22 (08) ◽  
pp. 1 ◽  
Author(s):  
Baowei Fei ◽  
Guolan Lu ◽  
Xu Wang ◽  
Hongzheng Zhang ◽  
James V. Little ◽  
...  
Keyword(s):  
Pilot Study ◽  
Cancer Patients ◽  
Hyperspectral Imaging ◽  
Label Free ◽  
Margin Assessment ◽  
Tumor Margin
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