Influence of substrate stiffness on the cellular mechanical properties

2020 ◽  
Author(s):  
Xiaoqiong Tang ◽  
Jiangbing Mao ◽  
Yuhua Wang ◽  
WeiWei Ruan ◽  
Hongqin Yang
Keyword(s):  
Mechanical Properties ◽  
Substrate Stiffness ◽  
Influence Of Substrate

scholarly journals Modifying the nanostructure and the mechanical properties of Mo2BC hard coatings: Influence of substrate temperature during magnetron sputtering

2018 ◽  
Vol 142 ◽  
pp. 203-211 ◽  
Author(s):  
Stephan Gleich ◽  
Rafael Soler ◽  
Hanna Fager ◽  
Hamid Bolvardi ◽  
Jan-Ole Achenbach ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Magnetron Sputtering ◽  
Substrate Temperature ◽  
Hard Coatings ◽  
Influence Of Substrate

scholarly journals Substrate stiffness induced mechanotransduction regulates temporal evolution of human fetal neural progenitor cell phenotype, differentiation, and biomechanics

2020 ◽  
Vol 8 (19) ◽  
pp. 5452-5464
Author(s):  
Chandrasekhar Kothapalli ◽  
Gautam Mahajan ◽  
Kurt Farrell
Keyword(s):  
Mechanical Properties ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
Progenitor Cell ◽  
Temporal Evolution ◽  
Neural Progenitor Cell ◽  
Neural Progenitor ◽  
Substrate Stiffness ◽  
Cell Phenotype ◽  
Spatiotemporal Evolution

We here report on the substrate stiffness dependent spatiotemporal evolution of mechanical properties of neural stem cells and their progenies.

scholarly journals Atomic force acoustic microscopy reveals the influence of substrate stiffness and topography on cell behavior

2019 ◽  
Vol 10 ◽  
pp. 2329-2337 ◽  
Author(s):  
Yan Liu ◽  
Li Li ◽  
Xing Chen ◽  
Ying Wang ◽  
Meng-Nan Liu ◽  
...  
Keyword(s):  
Acoustic Microscopy ◽  
Substrate Stiffness ◽  
Cell Behavior ◽  
Cell Alignment ◽  
Nondestructive Technique ◽  
Elastic Deformations ◽  
L929 Cells ◽  
Atomic Force ◽  
Cell Substrate ◽  
Influence Of Substrate

The stiffness and the topography of the substrate at the cell–substrate interface are two key properties influencing cell behavior. In this paper, atomic force acoustic microscopy (AFAM) is used to investigate the influence of substrate stiffness and substrate topography on the responses of L929 fibroblasts. This combined nondestructive technique is able to characterize materials at high lateral resolution. To produce substrates of tunable stiffness and topography, we imprint nanostripe patterns on undeveloped and developed SU-8 photoresist films using electron-beam lithography (EBL). Elastic deformations of the substrate surfaces and the cells are revealed by AFAM. Our results show that AFAM is capable of imaging surface elastic deformations. By immunofluorescence experiments, we find that the L929 cells significantly elongate on the patterned stiffness substrate, whereas the elasticity of the pattern has only little effect on the spreading of the L929 cells. The influence of the topography pattern on the cell alignment and morphology is even more pronounced leading to an arrangement of the cells along the nanostripe pattern. Our method is useful for the quantitative characterization of cell–substrate interactions and provides guidance for the tissue regeneration therapy in biomedicine.

Substrate Stiffness Affects Laminin-332 Matrix Deposition in Cultured Keretinocytes

2007 ◽  
Author(s):  
Abel L. Thangawng ◽  
Rodney S. Ruoff ◽  
Jonathan C. Jones ◽  
Matthew R. Glucksberg
Keyword(s):  
Mechanical Properties ◽  
Extracellular Matrix ◽  
Cell Motility ◽  
Substrate Stiffness ◽  
Mechanical Environment ◽  
Matrix Deposition ◽  
Laminin 332 ◽  
Substrate Influence

It has been reported that the mechanical properties of a substrate influence cell motility, morphology, and adhesion [1–3]. This work is an attempt to move a step further beyond cells’ sensing the mechanical properties of their environment, by determining whether the secretion and assembly of laminin extracellular matrix is regulated by the mechanical environment in which the cell is placed. We hypothesize that this matrix then influences the behavior of the cell, particularly with regard to its motility.

Substrate stiffness tunes the dynamics of polyvalent rolling motors

Soft Matter ◽  
2021 ◽  
Author(s):  
Chapin S. Korosec ◽  
Lavisha Jindal ◽  
Mathew Schneider ◽  
Ignacio Calderon de la Barca ◽  
Martin J. Zuckermann ◽  
...  
Keyword(s):  
Substrate Stiffness ◽  
Active Motion ◽  
Influence Of Substrate

Nature utilizes the burnt bridges ratchet (BBR) to generate active motion in a variety of biological contexts. Here, the influence of substrate stiffness on spherical BBR dynamics is investigated.

scholarly journals Influence of the PDMS substrate stiffness on the adhesion of Acanthamoeba castellanii

2014 ◽  
Vol 5 ◽  
pp. 1393-1398 ◽  
Author(s):  
Sören B Gutekunst ◽  
Carsten Grabosch ◽  
Alexander Kovalev ◽  
Stanislav N Gorb ◽  
Christine Selhuber-Unkel
Keyword(s):  
Cell Adhesion ◽  
Elastic Properties ◽  
Mammalian Cells ◽  
Cellular Response ◽  
Acanthamoeba Castellanii ◽  
Substrate Stiffness ◽  
Human Pathogen ◽  
Human Pathogens ◽  
Young’S Moduli ◽  
Influence Of Substrate

Background: Mechanosensing of cells, particularly the cellular response to substrates with different elastic properties, has been discovered in recent years, but almost exclusively in mammalian cells. Much less attention has been paid to mechanosensing in other cell systems, such as in eukaryotic human pathogens. Results: We report here on the influence of substrate stiffness on the adhesion of the human pathogen Acanthamoebae castellanii (A. castellanii). By comparing the cell adhesion area of A. castellanii trophozoites on polydimethylsiloxane (PDMS) substrates with different Young’s moduli (4 kPa, 29 kPa, and 128 kPa), we find significant differences in cell adhesion area as a function of substrate stiffness. In particular, the cell adhesion area of A. castellanii increases with a decreasing Young’s modulus of the substrate. Conclusion: The dependence of A. castellanii adhesion on the elastic properties of the substrate is the first study suggesting a mechanosensory effect for a eukaryotic human pathogen. Interestingly, the main targets of A. castellanii infections in the human body are the eye and the brain, i.e., very soft environments. Thus, our study provides first hints towards the relevance of mechanical aspects for the pathogenicity of eukaryotic parasites.

The effect of substrate bias on the characteristics of CrN coatings deposited by DC-superimposed HiPIMS system

2017 ◽  
Vol 31 (16-19) ◽  
pp. 1744032 ◽  
Author(s):  
X. Zuo ◽  
F. Xia ◽  
D. Zhang ◽  
P. L. Ke ◽  
Q. M. Wang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Bias Voltage ◽  
Indentation Test ◽  
Grain Structure ◽  
Substrate Bias ◽  
Cross Sectional ◽  
Microstructure And Mechanical Properties ◽  
Columnar Grain ◽  
Influence Of Substrate ◽  
Crn Coatings

Chromium nitride coatings were prepared by reactive DC-superimposed high-power-impulse magnetron sputtering (HiPIMS) system. The influence of substrate bias on the microstructure and mechanical properties of CrN coatings was investigated. XRD and cross-sectional SEM were utilized to characterize the film structures. Mechanical properties were characterized by nanoindentation and Vickers indentation test. The results revealed that the microstructure and mechanical properties of CrN coatings were affected by bias voltage. The CrN coatings exhibited dense and fine columnar grain structure with the hardness of about 18.7 GPa. The fracture toughness of CrN coatings was around 3.16 MPa ⋅ m[Formula: see text]. However, further increase of the bias voltage from −250 V to −300 V led to the degradation of coating properties.

The influence of substrate stiffness gradients on primary human dermal fibroblasts

Biomaterials ◽  
2013 ◽  
Vol 34 (21) ◽  
pp. 5070-5077 ◽  
Author(s):  
Isabel Hopp ◽  
Andrew Michelmore ◽  
Louise E. Smith ◽  
David E. Robinson ◽  
Akash Bachhuka ◽  
...  
Keyword(s):  
Dermal Fibroblasts ◽  
Substrate Stiffness ◽  
Human Dermal Fibroblasts ◽  
Influence Of Substrate

scholarly journals Astrogliosis in a dish: substrate stiffness induces astrogliosis in primary rat astrocytes

RSC Advances ◽  
2016 ◽  
Vol 6 (41) ◽  
pp. 34447-34457 ◽  
Author(s):  
Christina L. Wilson ◽  
Stephen L. Hayward ◽  
Srivatsan Kidambi
Keyword(s):  
Mechanical Properties ◽  
Substrate Stiffness ◽  
Cellular Microenvironment ◽  
Rat Astrocytes

Mechanical properties of the cellular microenvironment induces astrogliosisin vitroin primary rat astrocytes.

Sign in / Sign up

Export Citation Format

Share Document