Raman imaging for detection of corn kernels infected with Aspergillus flavus: a preliminary study

2020 ◽  
Author(s):  
Feifei Tao ◽  
Haibo Yao ◽  
Zuzana Hruska ◽  
Kanniah Rajasekaran ◽  
Jianwei Qin ◽  
...  
Keyword(s):  
Aspergillus Flavus ◽  
Raman Imaging ◽  
Preliminary Study ◽  
Corn Kernels

The preliminary study of mirna exploration in Aspergillus flavus

Toxicon ◽  
2019 ◽  
Vol 158 ◽  
pp. S58
Author(s):  
Mingzhu Li ◽  
Youhuang Bai ◽  
Shihua Wang
Keyword(s):  
Aspergillus Flavus ◽  
Preliminary Study

scholarly journals Resistance to Aspergillus flavus in Corn Kernels Is Associated with a 14-kDa Protein

1998 ◽  
Vol 88 (4) ◽  
pp. 276-281 ◽  
Author(s):  
Z.-Y. Chen ◽  
R. L. Brown ◽  
A. R. Lax ◽  
B. Z. Guo ◽  
T. E. Cleveland ◽  
...  
Keyword(s):  
Aspergillus Flavus ◽  
Trypsin Inhibitor ◽  
Aflatoxin Contamination ◽  
Inhibitor Protein ◽  
Trypsin Activity ◽  
Terminal Sequence ◽  
High Concentration ◽  
Resistant Varieties ◽  
Corn Kernels

Corn genotypes resistant or susceptible to Aspergillus flavus were extracted for protein analysis using a pH 2.8 buffer. The profile of protein extracts revealed that a 14-kDa protein is present in relatively high concentration in kernels of seven resistant corn genotypes, but is absent or present only in low concentration in kernels of six susceptible ones. The N-terminal sequence of this 14-kDa protein showed 100% homology to a corn trypsin inhibitor. The 14-kDa protein purified from resistant varieties also demonstrated in vitro inhibition of both trypsin activity and the growth of A. flavus. This is the first demonstration of antifungal activity of a corn 14-kDa trypsin inhibitor protein. The expression of this protein among tested genotypes may be related to their difference in resistance to A. flavus infection and subsequent aflatoxin contamination.

scholarly journals Lack of Host Specialization inAspergillus flavus

2000 ◽  
Vol 66 (1) ◽  
pp. 320-324 ◽  
Author(s):  
Raymond J. St. Leger ◽  
Steven E. Screen ◽  
Bijan Shams-Pirzadeh
Keyword(s):  
Aspergillus Fumigatus ◽  
Aspergillus Nidulans ◽  
Aspergillus Flavus ◽  
Galleria Mellonella ◽  
Host Specialization ◽  
Plant Tissues ◽  
Animal Hosts ◽  
Insect Hemocytes ◽  
Corn Kernels ◽  
Bean Leaves

ABSTRACT Aspergillus spp. cause disease in a broad range of organisms, but it is unknown if strains are specialized for particular hosts. We evaluated isolates of Aspergillus flavus,Aspergillus fumigatus, and Aspergillus nidulansfor their ability to infect bean leaves, corn kernels, and insects (Galleria mellonella). Strains of A. flavus did not affect nonwounded bean leaves, corn kernels, or insects at 22°C, but they killed insects following hemocoelic challenge and caused symptoms ranging from moderate to severe in corn kernels and bean leaves injured during inoculation. The pectinase P2c, implicated in aggressive colonization of cotton bolls, is produced by most A. flavus isolates, but its absence did not prevent colonization of bean leaves. Proteases have been implicated in colonization of animal hosts. All A. flavus strains produced very similar patterns of protease isozymes when cultured on horse lung polymers. Quantitative differences in protease levels did not correlate with the ability to colonize insects. In contrast to A. flavus, strains ofA. nidulans and A. fumigatus could not invade living insect or plant tissues or resist digestion by insect hemocytes. Our results indicate that A. flavus has parasitic attributes that are lacking in A. fumigatus and A. nidulans but that individual strains of A. flavus are not specialized to particular hosts.

scholarly journals Antifungal activity of Cymbopogon citratus essential oil against Aspergillus flavus

2019 ◽  
Vol 41 ◽  
pp. 20
Author(s):  
Ana Paula Martinazzo ◽  
Filipe Da Silva de Oliveira ◽  
Carlos Eduardo de Souza Teodoro
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Essential Oil ◽  
Aspergillus Flavus ◽  
Inhibitory Concentration ◽  
Fungal Growth ◽  
In Vitro Tests ◽  
Cymbopogon Citratus ◽  
Object Of Study ◽  
Corn Kernels

The search for alternatives for the control of microbiological contamination in foods has been the object of study in different scientific areas. This study aimed to evaluate the efficiency of lemon grass (Cymbopogon citratus) essential oil in controlling the growth of the fungus Aspergillus flavus in three types of analysis: first, by in vitro tests, in essential oil doses between 0.2 and 1.0 μL/ml; second, by serial microdilution to determine the minimum inhibitory concentration, in doses between 0.1 and 1.2 μL/mL; and third, by inhibition of fungal growth in corn kernels contaminated using essential oil doses of 0.4, 0.7, and 1.0 μL/mL, in the incubation times of 14, 28, and 42 days. The in vitro tests showed that the essential oil controlled the fungus from doses of 0.6 μL/mL, but the dose of 1.0 μL/mL controlled 100% growth until day eight of incubation, from which it decreased. The minimum inhibitory concentration for the microdilution analysis was 0.9 μL/mL. The evaluation of the corn kernels for all doses of essential oil and times tested showed 100% inhibition of the fungal growth.

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