Determination of the diffusion coefficient of rivanol in dentin of a human tooth in vitro

2020 ◽  
Author(s):  
Alexey Selifonov ◽  
Valery Tuchin
Keyword(s):  
Diffusion Coefficient ◽  
Human Tooth

scholarly journals Определение коэффициента диффузии растворов метиленового синего в дентине зуба человека с помощью спектроскопии отражения и их антибактериальная активность при лазерном воздействии-=SUP=-*-=/SUP=-

2019 ◽  
Vol 126 (6) ◽  
pp. 832
Author(s):  
А.А. Селифонов ◽  
О.Г. Шаповал ◽  
А.Н. Микеров ◽  
В.В. Тучин
Keyword(s):  
Aqueous Solution ◽  
Methylene Blue ◽  
Laser Radiation ◽  
Micellar Solution ◽  
Cetylpyridinium Chloride ◽  
Suppressive Effect ◽  
Human Tooth ◽  
Solution Studies

The work is devoted to the determination of the diffusion coefficients of methylene blue in pure aqueous solution and in a micellar solution of a cationic surfactant in human tooth dentinal sections in vitro using diffuse reflectance optical spectroscopy and the free diffusion model. The determination of the diffusion coefficient of methylene blue for an aqueous solution is (6.74 ± 1.32) • 10−6 cm2/s and (1.93 ± 0.24) • 10−6 cm2/s for methylene blue in micellar solution. Studies of toxicity in daylight in the absence of laser radiation of methylene blue solutions in water and in solution of cetylpyridinium chloride, as well as the photodynamic effect of laser radiation (662 nm) on cells of Candida albicans, Staphylococcus aureus FDA 209P and Lactobacillus were carried out. The effect of laser radiation has a pronounced suppressive effect on all the studied microbial strains.

Determination of in vitro „anti-aging„-effects of Ganoderma pfeifferi

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
W Jülich ◽  
J Pörksen ◽  
H Welzel ◽  
U Lindequist
Keyword(s):  
Aging Effects

In vitro determination of the skin anti-aging potential of eleven South African plants

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
GN Ndlovu ◽  
G Fouche ◽  
W Cordier ◽  
V Steenkamp ◽  
M Tselanyane
Keyword(s):  
South African ◽  
South African Plants ◽  
African Plants

A Molecular Approach to Immunoscintigraphy: A Study of the T-Antigen Conformation on the Surface of Tumors

1987 ◽  
Vol 26 (01) ◽  
pp. 1-6 ◽  
Author(s):  
S. Selvaraj ◽  
M. R. Suresh ◽  
G. McLean ◽  
D. Willans ◽  
C. Turner ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Tumor Cell ◽  
T Antigen ◽  
Human Carcinomas ◽  
Animal Tumor ◽  
Synthetic Antigens

The role of glycoconjugates in tumor cell differentiation has been well documented. We have examined the expression of the two anomers of the Thomsen-Friedenreich antigen on the surface of human, canine and murine tumor cell membranes both in vitro and in vivo. This has been accomplished through the synthesis of the disaccharide terminal residues in both a and ß configuration. Both entities were used to generate murine monoclonal antibodies which recognized the carbohydrate determinants. The determination of fine specificities of these antibodies was effected by means of cellular uptake, immunohistopathology and immunoscintigraphy. Examination of pathological specimens of human and canine tumor tissue indicated that the expressed antigen was in the β configuration. More than 89% of all human carcinomas tested expressed the antigen in the above anomeric form. The combination of synthetic antigens and monoclonal antibodies raised specifically against them provide us with invaluable tools for the study of tumor marker expression in humans and their respective animal tumor models.

Storage of Human Blood Platelets

1974 ◽  
Vol 32 (02/03) ◽  
pp. 405-416 ◽  
Author(s):  
M. R Hardeman ◽  
Carina J L. Heynens
Keyword(s):  
Storage Temperature ◽  
Platelet Rich Plasma ◽  
Blood Platelets ◽  
Storage Period ◽  
Serotonin Uptake ◽  
Hypotonic Shock ◽  
Glycolytic Intermediates

SummaryStorage experiments were performed at 4°, 25° and 37° C with platelet-rich plasma under sterile conditions. In some experiments also the effect of storing platelets at 4° C in whole blood was investigated.Before, during and after three days of storage, the platelets were tested at 37° C for their serotonin uptake and response to hypotonic shock. In addition some glycolytic intermediates were determined.A fair correlation was noticed between the serotonin uptake and hypotonic shock experiments. Both parameters were best maintained at 25° C. Also platelet counting, performed after the storage period, indicated 25° C as the best storage temperature. Determination of glycolytic intermediates did not justify any conclusion regarding the optimal storage temperature. Of the various anticoagulants studied, ACD and heparin gave the best results as to the serotonin uptake and hypotonic shock response, either with fresh or stored platelets. The use of EDTA resulted in the lowest activity, especially after storage.The results of these storage experiments in vitro, correspond well with those in vivo reported in the literature.

Sign in / Sign up

Export Citation Format

Share Document