Integration of optical manipulation in 3D printed microfluidic devices (Conference Presentation)

2020 ◽  
Author(s):  
Haoran Wang ◽  
Anton Enders ◽  
Alexander Heisterkamp ◽  
Janina Bahnemann ◽  
Maria Leilani Torres-Mapa
Keyword(s):  
Microfluidic Devices ◽  
Optical Manipulation ◽  
3D Printed

scholarly journals 3D printed microfluidic lab-on-a-chip device for fiber-based dual beam optical manipulation

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Haoran Wang ◽  
Anton Enders ◽  
John-Alexander Preuss ◽  
Janina Bahnemann ◽  
Alexander Heisterkamp ◽  
...  
Keyword(s):  
Optical Fibers ◽  
Single Cells ◽  
Lab On A Chip ◽  
Cost Effective ◽  
Optical Manipulation ◽  
Hydrodynamic Focusing ◽  
Trapping Region ◽  
Dual Beam ◽  
3D Printed ◽  
On Chip

Abstract3D printing of microfluidic lab-on-a-chip devices enables rapid prototyping of robust and complex structures. In this work, we designed and fabricated a 3D printed lab-on-a-chip device for fiber-based dual beam optical manipulation. The final 3D printed chip offers three key features, such as (1) an optimized fiber channel design for precise alignment of optical fibers, (2) an optically clear window to visualize the trapping region, and (3) a sample channel which facilitates hydrodynamic focusing of samples. A square zig–zag structure incorporated in the sample channel increases the number of particles at the trapping site and focuses the cells and particles during experiments when operating the chip at low Reynolds number. To evaluate the performance of the device for optical manipulation, we implemented on-chip, fiber-based optical trapping of different-sized microscopic particles and performed trap stiffness measurements. In addition, optical stretching of MCF-7 cells was successfully accomplished for the purpose of studying the effects of a cytochalasin metabolite, pyrichalasin H, on cell elasticity. We observed distinct changes in the deformability of single cells treated with pyrichalasin H compared to untreated cells. These results demonstrate that 3D printed microfluidic lab-on-a-chip devices offer a cost-effective and customizable platform for applications in optical manipulation.

Automated calibration of 3D-printed microfluidic devices based on computer vision

2021 ◽  
Vol 15 (2) ◽  
pp. 024102
Author(s):  
Junchao Wang ◽  
Kaicong Liang ◽  
Naiyin Zhang ◽  
Hailong Yao ◽  
Tsung-Yi Ho ◽  
...  
Keyword(s):  
Computer Vision ◽  
Microfluidic Devices ◽  
Automated Calibration ◽  
3D Printed

scholarly journals Rapid Production of PDMS Microdevices for Electrodriven Separations and Microfluidics by 3D-Printed Scaffold Removal

Separations ◽  
2021 ◽  
Vol 8 (5) ◽  
pp. 67
Author(s):  
Alena Šustková ◽  
Klára Konderlová ◽  
Ester Drastíková ◽  
Stefan Sützl ◽  
Lenka Hárendarčíková ◽  
...  
Keyword(s):  
Organic Dyes ◽  
Microfluidic Devices ◽  
Proof Of Concept ◽  
Magnetic Microparticles ◽  
Mechanical Removal ◽  
Glass Slides ◽  
Rapid Production ◽  
3D Printed

In our work, we produced PDMS-based microfluidic devices by mechanical removal of 3D-printed scaffolds inserted in PDMS. Two setups leading to the fabrication of monolithic PDMS-based microdevices and bonded (or stamped) PDMS-based microdevices were designed. In the monolithic devices, the 3D-printed scaffolds were fully inserted in the PDMS and then carefully removed. The bonded devices were produced by forming imprints of the 3D-printed scaffolds in PDMS, followed by bonding the PDMS parts to glass slides. All these microfluidic devices were then successfully employed in three proof-of-concept applications: capture of magnetic microparticles, formation of droplets, and isotachophoresis separation of model organic dyes.

scholarly journals 3D printed microfluidic devices with immunoaffinity monoliths for extraction of preterm birth biomarkers

2018 ◽  
Vol 411 (21) ◽  
pp. 5405-5413 ◽  
Author(s):  
Ellen K. Parker ◽  
Anna V. Nielsen ◽  
Michael J. Beauchamp ◽  
Haifa M. Almughamsi ◽  
Jacob B. Nielsen ◽  
...  
Keyword(s):  
Preterm Birth ◽  
Microfluidic Devices ◽  
3D Printed

3D printed microfluidic devices and applications

2021 ◽  
pp. 659-679
Author(s):  
Sui Ching Phung ◽  
Qingfu Zhu ◽  
Kimberly Plevniak ◽  
Mei He
Keyword(s):  
Microfluidic Devices ◽  
3D Printed

scholarly journals 3D Printed Microfluidics

2020 ◽  
Vol 13 (1) ◽  
pp. 45-65 ◽  
Author(s):  
Anna V. Nielsen ◽  
Michael J. Beauchamp ◽  
Gregory P. Nordin ◽  
Adam T. Woolley
Keyword(s):  
3D Printing ◽  
Early Stage ◽  
Three Dimensional ◽  
Microfluidic Devices ◽  
Fabrication Method ◽  
Size Scale ◽  
Additional Work ◽  
Fluidic Devices ◽  
3D Printed ◽  
Microfabrication Techniques

Traditional microfabrication techniques suffer from several disadvantages, including the inability to create truly three-dimensional (3D) architectures, expensive and time-consuming processes when changing device designs, and difficulty in transitioning from prototyping fabrication to bulk manufacturing. 3D printing is an emerging technique that could overcome these disadvantages. While most 3D printed fluidic devices and features to date have been on the millifluidic size scale, some truly microfluidic devices have been shown. Currently, stereolithography is the most promising approach for routine creation of microfluidic structures, but several approaches under development also have potential. Microfluidic 3D printing is still in an early stage, similar to where polydimethylsiloxane was two decades ago. With additional work to advance printer hardware and software control, expand and improve resin and printing material selections, and realize additional applications for 3D printed devices, we foresee 3D printing becoming the dominant microfluidic fabrication method.

scholarly journals Characterization of Soft Tooling Photopolymers and Processes for Micromixing Devices with Variable Cross-Section

Micromachines ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 970
Author(s):  
J. Israel Martínez-López ◽  
Héctor Andrés Betancourt Cervantes ◽  
Luis Donaldo Cuevas Iturbe ◽  
Elisa Vázquez ◽  
Edisson A. Naula ◽  
...  
Keyword(s):  
Flow Regime ◽  
Cross Section ◽  
Cross Sections ◽  
Microfluidic Devices ◽  
Variable Cross Section ◽  
Variable Cross ◽  
Multiple Cross ◽  
3D Printed

In this paper, we characterized an assortment of photopolymers and stereolithography processes to produce 3D-printed molds and polydimethylsiloxane (PDMS) castings of micromixing devices. Once materials and processes were screened, the validation of the soft tooling approach in microfluidic devices was carried out through a case study. An asymmetric split-and-recombine device with different cross-sections was manufactured and tested under different regime conditions (10 < Re < 70). Mixing performances between 3% and 96% were obtained depending on the flow regime and the pitch-to-depth ratio. The study shows that 3D-printed soft tooling can provide other benefits such as multiple cross-sections and other potential layouts on a single mold.

scholarly journals 3D-Printed Microfluidic Devices for Enhanced Online Sampling and Direct Optical Measurements

ACS Sensors ◽  
2020 ◽  
Vol 5 (7) ◽  
pp. 2044-2051
Author(s):  
Giraso Keza Monia Kabandana ◽  
Curtis G. Jones ◽  
Sahra Khan Sharifi ◽  
Chengpeng Chen
Keyword(s):  
Microfluidic Devices ◽  
Optical Measurements ◽  
3D Printed ◽  
Online Sampling
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