scholarly journals Fast volumetric mapping of human brain slices

2020 ◽  
Author(s):  
Luca Pesce ◽  
Annunziatina Laurino ◽  
Vladislav Gavryusev ◽  
Giacomo Mazzamuto ◽  
Giuseppe Sancataldo ◽  
...  
Keyword(s):  
Human Brain ◽  
Brain Slices

Visualization of the ischemic core on native human brain slices by potassium staining method

2010 ◽  
Vol 192 (1) ◽  
pp. 17-21 ◽  
Author(s):  
László Csiba ◽  
Szabolcs Farkas ◽  
József Kollár ◽  
Ervin Berényi ◽  
Katalin Nagy ◽  
...  
Keyword(s):  
Human Brain ◽  
Staining Method ◽  
Brain Slices ◽  
Ischemic Core

scholarly journals Multicellular ‘hotspots’ harbour high-grade potential in lower-grade gliomas

2021 ◽  
Author(s):  
Alastair J Kirby ◽  
José P Lavrador ◽  
Istvan Bodi ◽  
Francesco Vergani ◽  
Ranjeev Bhangoo ◽  
...  
Keyword(s):  
Human Brain ◽  
Brain Tissue ◽  
Aminolevulinic Acid ◽  
Ex Vivo ◽  
Brain Slices ◽  
Glioma Cells ◽  
Malignant Progression ◽  
Lower Grade ◽  
High Grade ◽  
Human Brain Tissue

Abstract Background Lower-grade gliomas may be indolent for many years before developing malignant behaviour. The reasons mechanisms underlying malignant progression remain unclear. Methods We collected blocks of live human brain tissue donated by people undergoing glioma resection. The tissue blocks extended through the peritumoral cortex and into the glioma. The living human brain tissue was cut into ex vivo brain slices and bathed in 5-aminolevulinic acid (5-ALA). High-grade glioma cells avidly take up 5-aminolevulinic acid (5-ALA) and accumulate high levels of the fluorescent metabolite, Protoporphyrin IX (PpIX). We exploited the PpIX fluorescence emitted by higher-grade glioma cells to investigate the earliest stages of malignant progression in lower-grade gliomas. Results We found sparsely-distributed ‘hot-spots’ of PpIX-positive cells in living lower-grade glioma tissue. Glioma cells and endothelial cells formed part of the PpIX hotspots. Glioma cells in PpIX hotspots were IDH1 mutant and expressed nestin suggesting they had acquired stem-like properties. Spatial analysis with 5-ALA conjugated quantum dots indicated that these glioma cells replicated adjacent to blood vessels. PpIX hotspots formed in the absence of angiogenesis. Conclusion Our data show that PpIX hotspots represent microdomains of cells with high-grade potential within lower-grade gliomas and identify locations where malignant progression could start.

Procedure for Staining Fixed Human Brain Slices

1986 ◽  
Vol 61 (2) ◽  
pp. 71-73 ◽  
Author(s):  
Matthew W. Heller ◽  
Susan L. Stoddard
Keyword(s):  
Human Brain ◽  
Brain Slices

scholarly journals Amyloid-β Oligomers Induce Differential Gene Expression in Adult Human Brain Slices

2012 ◽  
Vol 287 (10) ◽  
pp. 7436-7445 ◽  
Author(s):  
Adriano Sebollela ◽  
Leo Freitas-Correa ◽  
Fabio F. Oliveira ◽  
Andrea C. Paula-Lima ◽  
Leonardo M. Saraiva ◽  
...  
Keyword(s):  
Gene Expression ◽  
Human Brain ◽  
Differential Gene Expression ◽  
Brain Slices ◽  
Amyloid Β ◽  
Adult Human Brain ◽  
Adult Human ◽  
Differential Gene

scholarly journals Spontaneous glioma-induced seizures recorded in a living human brain tissue preparation

2019 ◽  
Vol 21 (Supplement_4) ◽  
pp. iv16-iv16
Author(s):  
Alastair Kirby ◽  
Jose Pedro Lavrador ◽  
Christian Brogna ◽  
Francesco Vergani ◽  
Bassel Zebian ◽  
...  
Keyword(s):  
Human Brain ◽  
Brain Tissue ◽  
Aminolevulinic Acid ◽  
Brain Slices ◽  
Low Grade ◽  
Tissue Preparation ◽  
Spontaneous Seizure ◽  
Human Brain Tissue ◽  
Who Grade ◽  
The Brain

Abstract Gliomas often present clinically with seizures. Tumour-associated seizures can be difficult to control with medication. A deeper understanding of the cellular mechanisms underlying tumour-associated seizures would provide a basis for developing new treatments. Here, we investigate epileptic discharges in peritumoral cortex using living human brain tissue donated by people having a craniotomy for glioma resection (REC approval, 18/SW/002). The brain tissue was cut into thin slices, which preserved the architecture of the glioma and the adjacent healthy brain. The brain slices were incubated in 5-aminolevulinic acid to make the glioma cells fluorescent. This enabled us to make electrophysiological recordings of brain activity across the boundary between glioma and brain. We recorded from brain slices of 5 participants with glioblastoma and 4 participants with oligodendroglioma (WHO grade II – III). Spontaneous “seizure-like” discharges were recorded in brain slices from 5/8 participants (3 GBM, 2 oligodendroglioma) who reported seizures and from one participant (GBM) who had not had any clinical seizures. Further analysis of the seizure-like discharges revealed that they could be subdivided into two distinct types based on the major frequencies in the discharge. We concluded that human brain slices from people with either a low-grade or a high-grade glioma can generate spontaneous seizure-like discharges. The living human brain tissue preparation gives us a platform to study the mechanisms of tumour-associated seizures and how abnormal neural activity affects glioma growth.

Modulation of K + -evoked [ 3 H]-noradrenaline release from rat and human brain slices by gabapentin: involvement of K ATP channels

2001 ◽  
Vol 363 (5) ◽  
pp. 537-542 ◽  
Author(s):  
Thomas M. Freiman ◽  
Juraj Kukolja ◽  
Jan Heinemeyer ◽  
Klaus Eckhardt ◽  
Heike Aranda ◽  
...  
Keyword(s):  
Human Brain ◽  
Noradrenaline Release ◽  
Brain Slices

K+-evoked [3H]-norepinephrine release in human brain slices from epileptic and non-epileptic patients is differentially modulated by gabapentin and pinacidil

2006 ◽  
Vol 55 (2) ◽  
pp. 204-210 ◽  
Author(s):  
Thomas M. Freiman ◽  
Rainer Surges ◽  
Juraj Kukolja ◽  
Jan Heinemeyer ◽  
Maximilian Klar ◽  
...  
Keyword(s):  
Human Brain ◽  
Brain Slices ◽  
Norepinephrine Release ◽  
Epileptic Patients

A comparative study of bioradiography in human brain slices and preoperative PET imaging

2007 ◽  
Vol 1142 ◽  
pp. 19-27 ◽  
Author(s):  
Toru Sasaki ◽  
Tadashi Nariai ◽  
Taketoshi Maehara ◽  
Katsushige Sato ◽  
Keiich Oda ◽  
...  
Keyword(s):  
Comparative Study ◽  
Human Brain ◽  
Pet Imaging ◽  
Brain Slices

scholarly journals P11.49 An electrophysiological signature of glioma infiltration in the ex vivo human brain

2019 ◽  
Vol 21 (Supplement_3) ◽  
pp. iii54-iii54
Author(s):  
A J Kirby ◽  
J P Lavrador ◽  
C Brogna ◽  
F Vergani ◽  
C Chandler ◽  
...  
Keyword(s):  
Human Brain ◽  
Brain Tissue ◽  
Brain Slices ◽  
Glioma Cells ◽  
Low Grade ◽  
Tissue Preparation ◽  
High Grade ◽  
Spontaneous Seizure ◽  
Human Brain Tissue ◽  
The Brain

Abstract BACKGROUND Invading glioma cells affect the physiological function of the peritumoural cortex. This may manifest clinically as seizures. Here, we investigate the effect the invading glioma cells on the electrophysiological signalling of the peritumoral cortex using living human brain tissue donated by people having a craniotomy for glioma resection (REC approval, 18/SW/002). MATERIAL AND METHODS The brain tissue was cut into thin slices, which preserved the architecture of the glioma and the adjacent healthy brain. The brain slices were incubated in 5-aminolevulinic acid to make the glioma cells fluorescent. We observed 5-ALA induced fluorescence in both low-grade and high-grade gliomas. This enabled us to make electrophysiological recordings of brain activity across the boundary between glioma and brain. RESULTS We recorded from brain slices of 5 participants with glioblastoma and 4 participants with oligodendroglioma (WHO grade II - III). Spontaneous “seizure-like” discharges were recorded in brain slices from 5/8 participants (3 GBM, 2 oligodendroglioma) who reported seizures and from one participant (GBM) who had not had any clinical seizures. Further analysis of the electrical discharges revealed that they could be subdivided into two distinct types based on the major frequencies in the discharge. CONCLUSION We concluded that human brain slices from people with either a low-grade or a high-grade glioma can generate spontaneous seizure-like discharges. This electrophysiological signature will be compared to infiltration and grade of the glioma cells in the donated sample. The living human brain tissue preparation gives us a platform to study the mechanisms of tumour-associated seizures and how abnormal neural activity affects glioma growth.

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