Optical tools for analyzing and controlling the brain (Conference Presentation)

2020 ◽  
Author(s):  
Edward S. Boyden
Keyword(s):  
Optical Tools ◽  
The Brain

scholarly journals Optical Approaches for Interrogating Neural Circuits Controlling Hormone Secretion

Endocrinology ◽  
2018 ◽  
Vol 159 (11) ◽  
pp. 3822-3833 ◽  
Author(s):  
Su Young Han ◽  
Jenny Clarkson ◽  
Richard Piet ◽  
Allan E Herbison
Keyword(s):  
Hormone Secretion ◽  
Functional Studies ◽  
Optical Tools ◽  
Neural Populations ◽  
Functional Investigation ◽  
Functional Circuitry ◽  
The Brain ◽  
Genetic Mouse Models

Abstract Developments in optical imaging and optogenetics are transforming the functional investigation of neuronal networks throughout the brain. Recent studies in the neuroendocrine field have used genetic mouse models combined with a variety of light-activated optical tools as well as GCaMP calcium imaging to interrogate the neural circuitry controlling hormone secretion. The present review highlights the benefits and caveats of these approaches for undertaking both acute brain slice and functional studies in vivo. We focus on the use of channelrhodopsin and the inhibitory optogenetic tools, archaerhodopsin and halorhodopsin, in addition to GCaMP imaging of individual cells in vitro and neural populations in vivo using fiber photometry. We also address issues around the use of genetic vs viral delivery of encoded proteins to specific Cre-expressing cell populations, their quantification, and the use of conscious vs anesthetized animal models. To date, optogenetics and GCaMP imaging have proven useful in dissecting functional circuitry within the brain and are likely to become essential investigative tools for deciphering the different neural networks controlling hormone secretion.

scholarly journals Awarded abstracts from 20th annual Julius Marmur Symposium

2017 ◽  
Vol 31 (1&2) ◽  
pp. 40
Author(s):  
Philip D. Campbell ◽  
Florence L. Marlow ◽  
Fanny Cazettes ◽  
Jose L. Pena ◽  
Veronika Miskolci ◽  
...  
Keyword(s):  
Immune Cells ◽  
Small Gtpases ◽  
Optical Tools ◽  
Sensory Uncertainty ◽  
The Brain

VITAL ROLES OF KINESINS IN DEVELOPMENT AND DISEASE IN ZEBRAFISH Philip D. Campbell. Mentor: Dr. Florence L. Marlow HOW THE BRAIN HANDLES SENSORY UNCERTAINTY Fanny Cazettes. Mentor: Dr. Jose L. Pena OPTICAL TOOLS TO STUDY THE ISOFORM-SPECIFIC ROLES OF SMALL GTPASES IN IMMUNE CELLS Veronika Miskolci. Mentor: Dr. Dianne Cox & Dr. Louis Hodgson NEUTROPHIL AGING IS REGULATED BY THE MICROBIOME Dachuan Zhang. Mentor: Dr. Paul S. Frenette

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