Improved functional imaging of ganglion neuronal and photoreceptor cell layers in living human retina (Conference Presentation)

2020 ◽  
Author(s):  
Gereon M. Hüttmann ◽  
Clara Pfäffle ◽  
Hendrik Spahr ◽  
Lisa Kutzner ◽  
Sazan Burhan ◽  
...  
Keyword(s):  
Functional Imaging ◽  
Photoreceptor Cell ◽  
Human Retina ◽  
Cell Layers

Functional imaging of neuronal layers in the human retina (Conference Presentation)

2020 ◽  
Author(s):  
Clara Pfäffle ◽  
Hendrik Spahr ◽  
Lisa Kutzner ◽  
Sazan Burhan ◽  
Felix Hilge ◽  
...  
Keyword(s):  
Functional Imaging ◽  
Human Retina

Simultaneous functional imaging of neuronal and photoreceptor layers in living human retina

2019 ◽  
Vol 44 (23) ◽  
pp. 5671 ◽  
Author(s):  
Clara Pfäffle ◽  
Hendrik Spahr ◽  
Lisa Kutzner ◽  
Sazan Burhan ◽  
Felix Hilge ◽  
...  
Keyword(s):  
Functional Imaging ◽  
Human Retina

scholarly journals Protein Kinase A in Human Retina: Differential Localization of Cβ, Cα, RIIα, and RIIβ in Photoreceptors Highlights Non-redundancy of Protein Kinase A Subunits

2021 ◽  
Vol 14 ◽  
Author(s):  
Jinae N. Roa ◽  
Yuliang Ma ◽  
Zbigniew Mikulski ◽  
Qianlan Xu ◽  
Ronit Ilouz ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Ganglion Cells ◽  
Splice Variants ◽  
Photoreceptor Cells ◽  
Human Retina ◽  
Photoreceptor Outer Segments ◽  
Neuronal Tissues ◽  
Cell Layers ◽  
Kinase A

Protein kinase A (PKA) signaling is essential for numerous processes but the subcellular localization of specific PKA regulatory (R) and catalytic (C) subunits has yet to be explored comprehensively. Additionally, the localization of the Cβ subunit has never been spatially mapped in any tissue even though ∼50% of PKA signaling in neuronal tissues is thought to be mediated by Cβ. Here we used human retina with its highly specialized neurons as a window into PKA signaling in the brain and characterized localization of PKA Cα, Cβ, RIIα, and RIIβ subunits. We found that each subunit presented a distinct localization pattern. Cα and Cβ were localized in all cell layers (photoreceptors, interneurons, retinal ganglion cells), while RIIα and RIIβ were selectively enriched in photoreceptor cells where both showed distinct patterns of co-localization with Cα but not Cβ. Only Cα was observed in photoreceptor outer segments and at the base of the connecting cilium. Cβ in turn, was highly enriched in mitochondria and was especially prominent in the ellipsoid of cone cells. Further investigation of Cβ using RNA BaseScope technology showed that two Cβ splice variants (Cβ4 and Cβ4ab) likely code for the mitochondrial Cβ proteins. Overall, our data indicates that PKA Cα, Cβ, RIIα, and RIIβ subunits are differentially localized and are likely functionally non-redundant in the human retina. Furthermore, Cβ is potentially important for mitochondrial-associated neurodegenerative diseases previously linked to PKA dysfunction.

The Ultrastructure of Monkey Gingival Epithelium

1967 ◽  
Vol 25 ◽  
pp. 16-17
Author(s):  
C.N. Sun
Keyword(s):  
Epithelial Cells ◽  
Macaca Nemestrina ◽  
Stratum Granulosum ◽  
Solution Ph ◽  
Gingival Epithelial Cells ◽  
Stratum Spinosum ◽  
Cell Layers ◽  
Gingival Epithelium ◽  
The Individual ◽  
Different Thickness

The present study demonstrates the ultrastructure of the gingival epithelium of the pig tail monkey (Macaca nemestrina). Specimens were taken from lingual and facial gingival surfaces and fixed in Dalton's chrome osmium solution (pH 7.6) for 1 hr, dehydrated, and then embedded in Epon 812.Tonofibrils are variable in number and structure according to the different region or location of the gingival epithelial cells, the main orientation of which is parallel to the long axis of the cells. The cytoplasm of the basal epithelial cells contains a great number of tonofilaments and numerous mitochondria. The basement membrane is 300 to 400 A thick. In the cells of stratum spinosum, the tonofibrils are densely packed and increased in number (fig. 1 and 3). They seem to take on a somewhat concentric arrangement around the nucleus. The filaments may occur scattered as thin fibrils in the cytoplasm or they may be arranged in bundles of different thickness. The filaments have a diameter about 50 A. In the stratum granulosum, the cells gradually become flatted, the tonofibrils are usually thin, and the individual tonofilaments are clearly distinguishable (fig. 2). The mitochondria and endoplasmic reticulum are seldom seen in these superficial cell layers.

Morphological and elemental analysis of isolated incubated frog retina-choroid

1984 ◽  
Vol 42 ◽  
pp. 298-299
Author(s):  
B. J. Panessa-Warren ◽  
J. B. Warren ◽  
H. W. Kraner
Keyword(s):  
Elemental Analysis ◽  
Pigment Epithelium ◽  
Human Retina ◽  
Healthy Individuals ◽  
Anterior Portion ◽  
Pathological Conditions ◽  
Frog Retina ◽  
Isolated Retina ◽  
Vertebrate Eye ◽  
Retina Pigment Epithelium

Our previous studies have demonstrated that abnormally high amounts of calcium (Ca) and zinc (Zn) can be accumulated in human retina-choroid under pathological conditions and that barium (Ba), which was not detected in the eyes of healthy individuals, is deposited in the retina pigment epithelium (RPE), and to a lesser extent in the sensory retina and iris. In an attempt to understand how these cations can be accumulated in the vertebrate eye, a morphological and microanalytical study of the uptake and loss of specific cations (K, Ca,Ba,Zn) was undertaken with incubated Rana catesbiana isolated retina and RPE preparations. Large frogs (650-800 gms) were dark adapted, guillotined and their eyes enucleated in deep ruby light. The eyes were hemisected behind the ora serrata and the anterior portion of the eye removed. The eyecup was bisected along the plane of the optic disc and the two segments of retina peeled away from the RPE and incubated.

Abstract #1100: Tumor Induced Osteomalacia: Clinical; Biochemical and Radiological Features from a Single Tertiary Care Centre with Emphasis on Functional Imaging.

2017 ◽  
Vol 23 ◽  
pp. 289
Author(s):  
Vineet Surana ◽  
Rajesh Khadgawat ◽  
Nikhil Tandon ◽  
Chandrashekhar Bal ◽  
Kandasamy Devasenathipathy
Keyword(s):  
Functional Imaging ◽  
Tertiary Care ◽  
Care Centre ◽  
Tertiary Care Centre ◽  
Radiological Features

Noninvasive Assessment of Coronary Artery Disease – Anatomical versus Functional Imaging and the Marginal Role of Exercise Electrocardiograms

Praxis ◽  
2020 ◽  
Vol 109 (14) ◽  
pp. 1141-1149
Author(s):  
Martina Boscolo Berto ◽  
Dominik C. Benz ◽  
Christoph Gräni
Keyword(s):  
Coronary Artery Disease ◽  
Coronary Artery ◽  
Functional Imaging ◽  
Clinical Aspects ◽  
Industrialized Countries ◽  
Coronary Syndromes ◽  
Pros And Cons ◽  
Noninvasive Assessment ◽  
Artery Disease ◽  
European Society Of Cardiology

Abstract. Coronary artery disease (CAD) is the leading cause of morbidity and mortality in the industrialized countries. Assessment of symptomatic patients with suspected obstructive CAD is a common reason for a clinical visit. Noninvasive anatomical and functional imaging are established tools to rule-in and rule-out CAD, to assess the severity of disease and to determine the potential risk of future cardiovascular events. In this review, we discuss the updated Guidelines from the European Society of Cardiology on Chronic Coronary Syndromes and explore the different imaging modalities used in current clinical practice for the noninvasive assessment of CAD. The pros and cons of each method, especially comparing anatomical and functional testing, are presented. Furthermore we we address the practical clinical aspects in the selection of the optimal noninvasive tests according to clinical need.

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