2D image-guided cell sorter and 3D imaging flow cytometer

2020 ◽  
Author(s):  
Rui Tang ◽  
Xinyu Chen ◽  
Zunming Zhang ◽  
Lauren Waller ◽  
Jiajie Chen ◽  
...  
Keyword(s):  
3D Imaging ◽  
Flow Cytometer ◽  
Cell Sorter ◽  
Image Guided

PO-1300: Manufactured 3D imaging treatment table for image-guided brachytherapy

2020 ◽  
Vol 152 ◽  
pp. S686
Author(s):  
J. Papp ◽  
M. Simon ◽  
E. Csiki ◽  
I. Törő ◽  
Á. Kovács
Keyword(s):  
3D Imaging ◽  
Image Guided ◽  
Treatment Table

Image-guided microfluidic cell sorter with machine learning

2019 ◽  
Author(s):  
Yi Gu ◽  
Rui Tang ◽  
Alex Ce Zhang ◽  
Yuanyuan Han ◽  
Yu-Hwa Lo
Keyword(s):  
Machine Learning ◽  
Cell Sorter ◽  
Image Guided ◽  
Microfluidic Cell Sorter

scholarly journals A light sheet based high throughput 3D-imaging flow cytometer for phytoplankton analysis

2013 ◽  
Vol 21 (12) ◽  
pp. 14474 ◽  
Author(s):  
Jianglai Wu ◽  
Jianping Li ◽  
Robert K.Y. Chan
Keyword(s):  
High Throughput ◽  
3D Imaging ◽  
Light Sheet ◽  
Flow Cytometer

Usage of the flow cytometer-cell sorter

1981 ◽  
Vol 47 (1) ◽  
pp. 13-24 ◽  
Author(s):  
R.G. Miller ◽  
M.E. Lalande ◽  
M.J. McCutcheon ◽  
S.S. Stewart ◽  
G.B. Price
Keyword(s):  
Flow Cytometer ◽  
Cell Sorter

Graphical User Interface for Human Intervention in 2D-3D Registration of Medical Images

2013 ◽  
Author(s):  
Grant L. S. Marchelli ◽  
David R. Haynor ◽  
William R. Ledoux ◽  
Mark A. Ganter ◽  
Duane W. Storti
Keyword(s):  
User Interface ◽  
Graphical User Interface ◽  
3D Imaging ◽  
Quality Measure ◽  
Reference Image ◽  
3D Registration ◽  
Data Set ◽  
Image Guided ◽  
2D And 3D

Image-guided medical therapies and image-guided biomechanical measurement systems often combine 2D and 3D imaging modalities. Determination of relations between the 2D and 3D imaging data is known as 2D-3D registration. Motivated by an ongoing project aimed at non-invasive, marker-free measurement of the kinematics of the bones in the foot during gait, we consider a registration approach that involves (1) computing projections of the 3D data set, (2) computing a quality measure to describe the agreement/discrepancy between the simulated projections and actual 2D images, and (3) optimization of the quality measure relative to the kinematic degrees of freedom to determine the optimal registration. For our particular project, the 3D imaging modality is CT scan, the 2D modality is bi-plane fluoroscopy, the computed projection is a digitally reconstructed radiograph (DRR), the quality measure is normalized cross-correlation (NCC) between a pair of DRRs and a pair of corresponding fluoroscope images, and the 2D imaging includes a sequence of several hundred stereo image pairs. We have recently released a software toolkit, DRRACC, that accelerates both the DRR and NCC computations via GPU-based parallel processing to enable more efficient automated determination of kinematic relations for optimal registration. While fully automated 2D-3D registration is desirable, there are situations (such as creating a reasonable starting configuration for optimization, re-starting after the optimizer fails to converge, and visual verification of registration relations) when it is desirable/necessary to have a human in the loop. In this paper, we present an OpenGL-based graphical user interface that employs the DRRACC toolkit to allow the user to manipulate the kinematics of individual objects (bones) segmented from the 3D imaging and to view the corresponding DRR and the associated correlation with a reference image in real time. We also present plots showing initial results for the dependence of the registration measure on pairs of kinematic parameters. The plots show well-defined peaks that support the hope for automated registration, but they also contain large relatively flat regions that may prove problematic for gradient-based optimizers and necessitate the sort of interface presented in this paper.

Minimally invasive image-guided direct repair of bilateral L-5 pars interarticularis defects

2008 ◽  
Vol 25 (2) ◽  
pp. E13 ◽  
Author(s):  
Ryan P. Brennan ◽  
Philip Y. Smucker ◽  
Eric M. Horn
Keyword(s):  
Minimally Invasive ◽  
3D Imaging ◽  
The Novel ◽  
Open Technique ◽  
Conservative Care ◽  
Male Athlete ◽  
Intraoperative 3D Imaging ◽  
Image Guided ◽  
Lower Back ◽  
Pars Interarticularis

Lower back pain from spondylolysis historically has been treated with a variety of options ranging from conservative care to open fusion. The authors describe the novel technique of minimally invasive bilateral pars interarticularis screw placement by utilizing intraoperative 3D imaging and frameless navigation in a 17-year-old male athlete. This technique is a modification of the open technique first described in 1970 by Buck and has the advantages of minimal dissection requirements with improved screw trajectory visualization. The patient's postoperative course is discussed, followed by a brief literature review of pars interarticularis defect treatment.

scholarly journals Novel Impactor and Microsphere‐Based Assay Used to Measure Containment of Aerosols Generated in a Flow Cytometer Cell Sorter

2018 ◽  
Vol 95 (2) ◽  
pp. 173-182 ◽  
Author(s):  
Stephen P. Perfetto ◽  
Phillip J. Hogarth ◽  
Simon Monard ◽  
Ben Fontes ◽  
Kristen M. Reifel ◽  
...  
Keyword(s):  
Flow Cytometer ◽  
Cell Sorter

scholarly journals A low-voltage droplet charging circuit with simulative cell-sorting function for flow cytometer-cell sorter

Cytometry ◽  
2000 ◽  
Vol 39 (4) ◽  
pp. 306-309
Author(s):  
Hongwei Zhou ◽  
Bohai Lin ◽  
Wei Wu ◽  
Youling Zhang ◽  
Lihua Wang
Keyword(s):  
Cell Sorting ◽  
Low Voltage ◽  
Flow Cytometer ◽  
Cell Sorter

scholarly journals Evaluating optical filter efficiency in a flow cytometer.

1980 ◽  
Vol 28 (10) ◽  
pp. 1136-1137 ◽  
Author(s):  
M R Loken
Keyword(s):  
Scattered Light ◽  
Spectral Characteristics ◽  
Optical Filter ◽  
Optical Filters ◽  
Flow Cytometer ◽  
Operating Conditions ◽  
Fluorescent Light ◽  
Cell Sorter ◽  
Filter Efficiency ◽  
Normal Operating

A simple yet effective method of testing optical filters under normal operating conditions on a Fluorescence Activated Cell Sorter is described. The spectral characteristics of three filters are compared to demonstrate the methods of determining which filter is the best in a particular situation. These tests include determining the efficiency of transmitting fluorescent light, determining the efficiency of removing scattered light, and detecting fluorescence originating from the filter itself.

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