Fluorescence lifetime image scanning microscopy (Conference Presentation)

2020 ◽  
Author(s):  
Ingo Gregor ◽  
Niels Radmacher ◽  
Jörg Enderlein
Keyword(s):  
Fluorescence Lifetime ◽  
Scanning Microscopy ◽  
Image Scanning

scholarly journals Image Scanning Microscopy with Single-Photon Detector Array

2018 ◽  
Author(s):  
Marco Castello ◽  
Giorgio Tortarolo ◽  
Mauro Buttafava ◽  
Takahiro Deguchi ◽  
Federica Villa ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Single Photon ◽  
Laser Scanning Microscopy ◽  
Scanning Microscopy ◽  
Detector Array ◽  
Photon Detector ◽  
Single Photon Detector ◽  
Image Scanning ◽  
Reconstruction Methods ◽  
The Way

Image scanning microscopy (ISM) improves the spatial resolution of conventional confocal laser-scanning microscopy (CLSM), but current implementations reduce versatility and restrict its combination with fluorescence spectroscopy techniques, such as fluorescence lifetime. Here, we describe a natural design of ISM based on a fast single-photon detector array, which allows straightforward upgrade of an existing confocal microscope, without compromising any of its functionalities. In contrast to all-optical ISM implementations, our approach provides access to the raw scanned images, opening the way to adaptive reconstruction methods, capable of considering different imaging conditions and distortions. We demonstrate its utility in the context of fluorescence lifetime, deep, multicolor and live-cell imaging. This implementation will pave the way for a transparent and massive transition from conventional CLSM to ISM.confocal microscopy | time-resolved spectroscopy | image scanning microscopy | single-photon detector array

Practical aspects of super-resolution optical fluctuation image scanning microscopy (SOFISM)

2020 ◽  
Author(s):  
Adrian Makowski ◽  
Gur Lubin ◽  
Ron Tenne ◽  
Aleksandra Sroda ◽  
Uri Rossman ◽  
...  
Keyword(s):  
Super Resolution ◽  
Scanning Microscopy ◽  
Image Scanning

scholarly journals EASY TWO-PHOTON IMAGE-SCANNING MICROSCOPY WITH SPAD ARRAY AND BLIND IMAGE RECONSTRUCTION

2019 ◽  
Author(s):  
S. V. Koho ◽  
E. Slenders ◽  
G. Tortarolo ◽  
M. Castello ◽  
M. Buttafava ◽  
...  
Keyword(s):  
Image Reconstruction ◽  
Single Photon ◽  
Imaging Modality ◽  
Scanning Microscopy ◽  
Array Detector ◽  
Reconstruction Method ◽  
Image Scanning ◽  
Two Photon ◽  
Blind Image

ABSTRACTTwo-photon excitation (2PE) microscopy is the imaging modality of choice, when one desires to work with thick biological samples, possibly in-vivo. However, the resolution in two-photon microscopy is poor, below confocal microscopy, and the lack of an optical pinhole becomes apparent in complex samples as reduced quality of optical sectioning. Here, we propose a straightforward implementation of 2PE image scanning microscopy (2PE-ISM) that, by leveraging our recently introduced ISM platform – based on a new single-photon avalanche diode array detector – coupled with a novel blind image reconstruction method, is shown to improve the optical resolution, as well as the overall image quality in various test samples. Most importantly, our 2PE-ISM implementation requires no calibration or other input from the user – it works like any old and familiar two-photon system, but simply produces higher resolution images (in real-time). Making the complexity disappear, in our view, is the biggest novelty here, and the key for making 2PE-ISM mainstream.

scholarly journals A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM

2019 ◽  
Vol 16 (2) ◽  
pp. 175-178 ◽  
Author(s):  
Marco Castello ◽  
Giorgio Tortarolo ◽  
Mauro Buttafava ◽  
Takahiro Deguchi ◽  
Federica Villa ◽  
...  
Keyword(s):  
Super Resolution ◽  
Scanning Microscopy ◽  
Image Scanning

scholarly journals Super-resolution microscopy based on parallel detection

2019 ◽  
Vol 12 (06) ◽  
pp. 1950023
Author(s):  
Zhimin Zhang ◽  
Shaocong Liu ◽  
Liang Xu ◽  
Yubing Han ◽  
Cuifang Kuang ◽  
...  
Keyword(s):  
Cross Correlation ◽  
Signal To Noise Ratio ◽  
3D Structure ◽  
Super Resolution ◽  
Confocal Imaging ◽  
Scanning Microscopy ◽  
Resolution Limit ◽  
Image Scanning ◽  
Parallel Detection ◽  
Super Resolution Microscopy

Image scanning microscopy based on pixel reassignment can improve the confocal resolution limit without losing the image signal-to-noise ratio (SNR) greatly [C. J. R. Sheppard, “Super-resolution in confocal imaging,” Optik 80(2) 53–54 (1988). C. B. Müller, E. Jörg, “Image scanning microscopy, “Phys. Rev. Lett. 104(19) 198101 (2010). C. J. R. Sheppard, S. B. Mehta, R. Heintzmann, “Superresolution by image scanning microscopy using pixel reassignment,” Opt. Lett. 38(15) 2889–2892 (2013)]. Here, we use a tailor-made optical fiber and 19 avalanche photodiodes (APDs) as parallel detectors to upgrade our existing confocal microscopy, termed as parallel-detection super-resolution (PDSR) microscopy. In order to obtain the correct shift value, we use the normalized 2D cross correlation to calculate the shifting value of each image. We characterized our system performance by imaging fluorescence beads and applied this system to observing the 3D structure of biological specimen.

Doubling the resolution of a confocal spinning-disk microscope using image scanning microscopy

2020 ◽  
Vol 16 (1) ◽  
pp. 164-181
Author(s):  
Shun Qin ◽  
Sebastian Isbaner ◽  
Ingo Gregor ◽  
Jörg Enderlein
Keyword(s):  
Scanning Microscopy ◽  
Image Scanning ◽  
Spinning Disk

Image scanning microscopy

2019 ◽  
Vol 51 ◽  
pp. 74-83 ◽  
Author(s):  
Ingo Gregor ◽  
Jörg Enderlein
Keyword(s):  
Scanning Microscopy ◽  
Image Scanning

scholarly journals Rapid 3D image scanning microscopy with multi-spot excitation and double-helix point spread function detection

2018 ◽  
Vol 26 (18) ◽  
pp. 23585 ◽  
Author(s):  
Siwei Li ◽  
Jingjing Wu ◽  
Heng Li ◽  
Danying Lin ◽  
Bin Yu ◽  
...  
Keyword(s):  
Point Spread Function ◽  
Double Helix ◽  
Scanning Microscopy ◽  
3D Image ◽  
Image Scanning ◽  
Point Spread ◽  
Spread Function

scholarly journals Super-resolution enhancement by quantum image scanning microscopy

2018 ◽  
Vol 13 (2) ◽  
pp. 116-122 ◽  
Author(s):  
Ron Tenne ◽  
Uri Rossman ◽  
Batel Rephael ◽  
Yonatan Israel ◽  
Alexander Krupinski-Ptaszek ◽  
...  
Keyword(s):  
Resolution Enhancement ◽  
Super Resolution ◽  
Scanning Microscopy ◽  
Quantum Image ◽  
Image Scanning
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