Rapid detection of Escherichia coli in aqueous samples by metabolic induced pH

2020 ◽  
Author(s):  
Noah Baker ◽  
Leonard Y. Nelson ◽  
Eric J. Seibel
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Aqueous Samples

scholarly journals Development of an Immunomagnetic Bead-Immunoliposome Fluorescence Assay for Rapid Detection of Escherichia coli O157:H7 in Aqueous Samples and Comparison of the Assay with a Standard Microbiological Method

2005 ◽  
Vol 71 (4) ◽  
pp. 1856-1864 ◽  
Author(s):  
Thomas R. DeCory ◽  
Richard A. Durst ◽  
Scott J. Zimmerman ◽  
Linda A. Garringer ◽  
Gary Paluca ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Magnetic Separation ◽  
Rapid Detection ◽  
Fluorescence Assay ◽  
Aqueous Samples ◽  
Microbiological Method ◽  
Immunomagnetic Beads ◽  
Immunomagnetic Bead ◽  
E Coli ◽  
Sulforhodamine B

ABSTRACT The objective of this study was to develop and optimize a protocol for the rapid detection of Escherichia coli O157:H7 in aqueous samples by a combined immunomagnetic bead-immunoliposome (IMB/IL) fluorescence assay. The protocol consisted of the filtration or centrifugation of 30- to 100-ml samples followed by incubation of the filter membranes or pellet with anti-E. coli O157:H7 immunomagnetic beads in growth medium specific for E. coli O157:H7. The resulting E. coli O157:H7-immunomagnetic bead complexes were isolated by magnetic separation, washed, and incubated with sulforhodamine B-containing immunoliposomes specific for E. coli O157:H7; the final immunomagnetic bead-E. coli O157:H7-immunoliposome complexes were again isolated by magnetic separation, washed, and lysed with a n-octyl-β-d-glucopyranoside to release sulforhodamine B. The final protocol took less than 8 h to complete and had a detection limit of less than 1 CFU of E. coli O157:H7 per ml in various aqueous matrices, including apple juice and cider. To validate the protocol at an independent facility, 100-ml samples of groundwater with and without E. coli O157:H7 (15 CFU) were analyzed by a public health laboratory using the optimized protocol and a standard microbiological method. While the IMB/IL fluorescence assay was able to identify E. coli O157:H7-containing samples with 100% accuracy, the standard microbiological method was unable to distinguish E. coli O157:H7-spiked samples from negative controls without further extensive workup. These results demonstrate the feasibility of using immunomagnetic beads in combination with sulforhodamine B-encapsulating immunoliposomes for the rapid detection of E. coli O157:H7 in aqueous samples.

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

2021 ◽  
pp. 247263032098193
Author(s):  
Cheng Liu ◽  
Shuiqin Fang ◽  
Yachen Tian ◽  
Youxue Wu ◽  
Meijiao Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Foodborne Pathogen ◽  
Test Strip ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157 ◽  
Aggregation Induced Emission ◽  
E Coli

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

Application of Bacteriophage in Rapid Detection of Escherichia coli in Foods

2020 ◽  
Author(s):  
Wang Jun ◽  
Kanach Andrew ◽  
Han Rongwei ◽  
Applegate Bruce
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection

Rapid detection of cefotaxime-resistant Escherichia coli by LC–MS

2015 ◽  
Vol 305 (8) ◽  
pp. 860-864 ◽  
Author(s):  
A. Burrer ◽  
P. Findeisen ◽  
E. Jäger ◽  
B. Ghebremedhin ◽  
A. Grundt ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection
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