Highly inclined plane illumination microscopy with extended imaging depth (Conference Presentation)

Author(s):  
Jinhan Ren ◽  
Kyu Young Han
Keyword(s):  
2021 ◽  
Vol 33 (6) ◽  
pp. 061707
Author(s):  
Alexander E. Dubinov ◽  
Djamilya N. Iskhakova ◽  
Valeria A. Lyubimtseva

1997 ◽  
Vol 36 (2) ◽  
pp. 135-143 ◽  
Author(s):  
Bhabani Shankar Dandapat ◽  
Anadi Shankar Gupta

1935 ◽  
Vol 19 (2) ◽  
pp. 207-214 ◽  
Author(s):  
C. W. Brown ◽  
E. E. Ghiselli ◽  
F. M. Henry
Keyword(s):  

2015 ◽  
Vol 112 (42) ◽  
pp. 12938-12943 ◽  
Author(s):  
Tzer Han Tan ◽  
Jesse L. Silverberg ◽  
Daniela S. Floss ◽  
Maria J. Harrison ◽  
Christopher L. Henley ◽  
...  

Experimental studies show that plant root morphologies can vary widely from straight gravity-aligned primary roots to fractal-like root architectures. However, the opaqueness of soil makes it difficult to observe how environmental factors modulate these patterns. Here, we combine a transparent hydrogel growth medium with a custom built 3D laser scanner to directly image the morphology of Medicago truncatula primary roots. In our experiments, root growth is obstructed by an inclined plane in the growth medium. As the tilt of this rigid barrier is varied, we find Medicago transitions between randomly directed root coiling, sinusoidal root waving, and normal gravity-aligned morphologies. Although these root phenotypes appear morphologically distinct, our analysis demonstrates the divisions are less well defined, and instead, can be viewed as a 2D biased random walk that seeks the path of steepest decent along the inclined plane. Features of this growth response are remarkably similar to the widely known run-and-tumble chemotactic behavior of Escherichia coli bacteria, where biased random walks are used as optimal strategies for nutrient uptake.


Author(s):  
Kristie Huda ◽  
Kenneth F. Swan ◽  
Cecilia T. Gambala ◽  
Gabriella C. Pridjian ◽  
Carolyn L. Bayer

AbstractFunctional photoacoustic imaging of the placenta could provide an innovative tool to diagnose preeclampsia, monitor fetal growth restriction, and determine the developmental impacts of gestational diabetes. However, transabdominal photoacoustic imaging is limited in imaging depth due to the tissue’s scattering and absorption of light. The aim of this paper was to investigate the impact of geometry and wavelength on transabdominal light delivery. Our methods included the development of a multilayer model of the abdominal tissue and simulation of the light propagation using Monte Carlo methods. A bifurcated light source with varying incident angle of light, distance between light beams, and beam area was simulated to analyze the effect of light delivery geometry on the fluence distribution at depth. The impact of wavelength and the effects of variable thicknesses of adipose tissue and muscle were also studied. Our results showed that the beam area plays a major role in improving the delivery of light to deep tissue, in comparison to light incidence angle or distance between the bifurcated fibers. Longer wavelengths, with incident fluence at the maximum permissible exposure limit, also increases fluence within deeper tissue. We validated our simulations using a commercially available light delivery system and ex vivo human placental tissue. Additionally, we compared our optimized light delivery to a commercially available light delivery system, and conclude that our optimized geometry could improve imaging depth more than 1.6×, bringing the imaging depth to within the needed range for transabdominal imaging of the human placenta.


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