Fingerprint–to-CH stretch region high spectral resolution Stimulated Raman Scattering microscopy with an Acousto Optical Tunable Filter (Conference Presentation)

Author(s):  
Sergey P. Laptenok ◽  
Luca Genchi ◽  
Vijayakumar P. Rajamanickam ◽  
Andrea Bertoncini ◽  
Alessandro Giammona ◽  
...  
2019 ◽  
Vol 12 (9) ◽  
Author(s):  
Sergey P. Laptenok ◽  
Vijayakumar P. Rajamanickam ◽  
Luca Genchi ◽  
Tual Monfort ◽  
Yeonwoo Lee ◽  
...  

2021 ◽  
Vol 11 (9) ◽  
pp. 3903
Author(s):  
Luigi Sirleto ◽  
Rajeev Ranjan ◽  
Maria Antonietta Ferrara

In the last decade, stimulated Raman scattering (SRS) imaging has been demonstrated to be a powerful method for label-free, non-invasive mapping of individual species distributions in a multicomponent system. This is due to the chemical selectivity of SRS techniques and the linear dependence of SRS signals on the individual species concentrations. However, even if significant efforts have been made to improve spectroscopic coherent Raman imaging technology, what is the best way to resolve overlapped Raman bands in biological samples is still an open question. In this framework, spectral resolution, i.e., the ability to distinguish closely lying resonances, is the crucial point. Therefore, in this paper, the interplay among pump and Stokes bandwidths, the degree of chirp-matching and the spectral resolution of femtosecond stimulated Raman scattering microscopy are experimentally investigated and the separation of protein and lipid bands in the C-H region, which are of great interest in biochemical studies, is, in principle, demonstrated.


2021 ◽  
Vol 255 ◽  
pp. 11009
Author(s):  
Rajeev Ranjan ◽  
Maria Antonietta Ferrara ◽  
Luigi Sirleto

The simultaneous mapping and the specificity of different chemical species are desirable in several biological and biomedical applications. The stimulated Raman Scattering technique is a proven and well-established label-free method to map the distributions of individual species in a multi-component-based system due to the linear dependence of signals on concentration and its chemical selectivity. In this framework, spectral resolution, i.e., the ability to distinguish closely lying resonances, plays a fundamental role. Here in this work, the cross-correlation of Ti:Sa & OPO femtosecond laser beams in a stimulated Raman scattering microscope is measured. The separation between protein and lipid bands in the C-H region is important for biochemical research and is successfully classified.


2009 ◽  
Vol 24 (3) ◽  
pp. 563-566 ◽  
Author(s):  
Zheng-Ping WANG ◽  
Da-Wei HU ◽  
Huai-Jin ZHANG ◽  
Xin-Guang XU ◽  
Ji-Yang WANG ◽  
...  

1996 ◽  
Vol 24 (8) ◽  
pp. 906-909
Author(s):  
Akio MIYAMOTO ◽  
Hidetsugu YOSHIDA ◽  
Yusuke MORI ◽  
Takatomo SASAKI ◽  
Sadao NAKAI

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