Easy two-photon image-scanning microscopy with SPAD array and blind image reconstruction (Conference Presentation)

2020 ◽  
Author(s):  
Sami V. Koho ◽  
Eli Slenders ◽  
Giorgio Tortarolo ◽  
Marco Castello ◽  
Mauro Buttafava ◽  
...  
Keyword(s):  
Image Reconstruction ◽  
Scanning Microscopy ◽  
Image Scanning ◽  
Two Photon ◽  
Blind Image

scholarly journals EASY TWO-PHOTON IMAGE-SCANNING MICROSCOPY WITH SPAD ARRAY AND BLIND IMAGE RECONSTRUCTION

2019 ◽  
Author(s):  
S. V. Koho ◽  
E. Slenders ◽  
G. Tortarolo ◽  
M. Castello ◽  
M. Buttafava ◽  
...  
Keyword(s):  
Image Reconstruction ◽  
Single Photon ◽  
Imaging Modality ◽  
Scanning Microscopy ◽  
Array Detector ◽  
Reconstruction Method ◽  
Image Scanning ◽  
Two Photon ◽  
Blind Image

ABSTRACTTwo-photon excitation (2PE) microscopy is the imaging modality of choice, when one desires to work with thick biological samples, possibly in-vivo. However, the resolution in two-photon microscopy is poor, below confocal microscopy, and the lack of an optical pinhole becomes apparent in complex samples as reduced quality of optical sectioning. Here, we propose a straightforward implementation of 2PE image scanning microscopy (2PE-ISM) that, by leveraging our recently introduced ISM platform – based on a new single-photon avalanche diode array detector – coupled with a novel blind image reconstruction method, is shown to improve the optical resolution, as well as the overall image quality in various test samples. Most importantly, our 2PE-ISM implementation requires no calibration or other input from the user – it works like any old and familiar two-photon system, but simply produces higher resolution images (in real-time). Making the complexity disappear, in our view, is the biggest novelty here, and the key for making 2PE-ISM mainstream.

scholarly journals Two-photon image-scanning microscopy with SPAD array and blind image reconstruction

2020 ◽  
Vol 11 (6) ◽  
pp. 2905 ◽  
Author(s):  
Sami V. Koho ◽  
Eli Slenders ◽  
Giorgio Tortarolo ◽  
Marco Castello ◽  
Mauro Buttafava ◽  
...  
Keyword(s):  
Image Reconstruction ◽  
Scanning Microscopy ◽  
Image Scanning ◽  
Two Photon ◽  
Blind Image

Image formation in image scanning microscopy, including the case of two-photon excitation

2017 ◽  
Vol 34 (8) ◽  
pp. 1339 ◽  
Author(s):  
Colin J. R. Sheppard ◽  
Marco Castello ◽  
Giorgio Tortarolo ◽  
Giuseppe Vicidomini ◽  
Alberto Diaspro
Keyword(s):  
Image Formation ◽  
Scanning Microscopy ◽  
Image Scanning ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

scholarly journals Two-photon PSF-engineered image scanning microscopy

2019 ◽  
Vol 44 (4) ◽  
pp. 895 ◽  
Author(s):  
Omer Tzang ◽  
Dan Feldkhun ◽  
Anurag Agrawal ◽  
Alexander Jesacher ◽  
Rafael Piestun
Keyword(s):  
Scanning Microscopy ◽  
Image Scanning ◽  
Two Photon

scholarly journals Two-photon laser scanning microscopy with electrowetting-based prism scanning

2017 ◽  
Vol 8 (12) ◽  
pp. 5412 ◽  
Author(s):  
Omkar D. Supekar ◽  
Baris N. Ozbay ◽  
Mo Zohrabi ◽  
Philip D. Nystrom ◽  
Gregory L. Futia ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Scanning Microscopy ◽  
Two Photon
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