Clinical applicability of in vivo harmonic generation microscopy for the diagnosis and grading of actinic keratosis (Conference Presentation)

2020 ◽  
Author(s):  
Chi-Kuang Sun ◽  
Yi Pan ◽  
Pei-Che Wu ◽  
Sheng-Tse Chen ◽  
Yi-Hua Liao
Keyword(s):  
Harmonic Generation ◽  
Actinic Keratosis ◽  
Clinical Applicability

In vivo optical virtual biopsy of human oral cavity with harmonic generation microscopy

2010 ◽  
Author(s):  
M.-R. Tsai ◽  
S.-Y. Chen ◽  
D.-B. Shieh ◽  
P.-J. Lou ◽  
C.-K. Sun
Keyword(s):  
Oral Cavity ◽  
Harmonic Generation ◽  
Human Oral Cavity

scholarly journals Characterization of Collagen I Fiber Thickness, Density, and Orientation in the Human Skin In Vivo Using Second-Harmonic Generation Imaging

Photonics ◽  
2021 ◽  
Vol 8 (9) ◽  
pp. 404
Author(s):  
Marius Kröger ◽  
Johannes Schleusener ◽  
Sora Jung ◽  
Maxim E. Darvin
Keyword(s):  
Extracellular Matrix ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Skin Diseases ◽  
Second Harmonic ◽  
Skin Aging ◽  
Collagen I ◽  
Fast Fourier Transformation ◽  
Occurrence Matrix

The assessment of dermal alterations is necessary to monitor skin aging, cancer, and other skin diseases and alterations. The gold standard of morphologic diagnostics is still histopathology. Here, we proposed parameters to distinguish morphologically different collagen I structures in the extracellular matrix and to characterize varying collagen I structures in the skin with similar SAAID (SHG-to-AF Aging Index of Dermis, SHG—second-harmonic generation; AF—autofluorescence) values. Test datasets for the papillary and reticular extracellular matrix from images in 24 female subjects, 36 to 50 years of age, were generated. Parameters for SAAID, edge detection, and fast Fourier transformation directionality were determined. Additionally, textural analyses based on the grey level co-occurrence matrix (GLCM) were conducted. At first, changes in the GLCM parameters were determined in the native greyscale images and, furthermore, in the Hilbert-transformed images. Our results demonstrate a robust set of parameters for noninvasive in vivo classification for morphologically different collagen I structures in the skin, with similar and different SAAID values. We anticipate our method to enable an automated prevention and monitoring system with an age- and gender-specific algorithm.

scholarly journals Tissue engineering in urology

2013 ◽  
Vol 3 (5) ◽  
pp. 403 ◽  
Author(s):  
Derek J. Matoka ◽  
Earl Y. Cheng
Keyword(s):  
Tissue Engineering ◽  
Multidisciplinary Approach ◽  
Normal Function ◽  
Healthy Tissue ◽  
Clinical Applicability ◽  
Basic Technology ◽  
And Function

Tissue engineering encompasses a multidisciplinary approach gearedtoward the development of biological substitutes designed to restoreand maintain normal function in diseased or injured tissues. Thisarticle reviews the basic technology that is used to generateimplantable tissue-engineered grafts in vitro that will exhibit characteristicsin vivo consistent with the physiology and function ofthe equivalent healthy tissue. We also examine the current trendsin tissue engineering designed to tailor scaffold construction, promoteangiogenesis and identify an optimal seeded cell source.Finally, we describe several currently applied therapeutic modalitiesthat use a tissue-engineered construct. While notable progresshas clearly been demonstrated in this emerging field, these effortshave not yet translated into widespread clinical applicability. Withcontinued development and innovation, there is optimism that thetremendous potential of this field will be realized.L’ingénierie tissulaire englobe une approche multidisciplinaireaxée sur le développement de substituts biologiques en vue derétablir et de maintenir la fonction normale de tissus lésés. L’articlequi suit passe en revue la technologie fondamentale utilisée pourgénérer des greffons implantables produits par ingénierie in vitroet possédant des caractéristiques in vivo correspondant aux tissussains équivalents sur les plans physiologique et fonctionnel.Nous examinons également les tendances actuelles en ingénierietissulaire visant à adapter des échafaudages tissulaires, à promouvoirl’angiogenèse et à dégager une source optimale de cellulesimplantables. Enfin, nous décrivons plusieurs modalités thérapeutiquesactuellement mises en application utilisant un échafaudagecréé par ingénierie tissulaire. En dépit de progrès remarquablesdans ce domaine en effervescence, les efforts déployés ne se sontpas encore traduits par une applicabilité clinique étendue. Desdéveloppements et des percées continus permettent d’être optimisteface au potentiel prodigieux de ce domaine.

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