Towards an active micropump-mixer for rapid anti-platelet drug screening in whole blood

2019 ◽  
Author(s):  
Crispin Szydzik ◽  
Rose J. Brazilek ◽  
Farzan Akbaridoust ◽  
Charitha de Silva ◽  
Mitchell Moon ◽  
...  
Keyword(s):  
Drug Screening ◽  
Whole Blood ◽  
Anti Platelet Drug

Whole Blood Deproteinization for Drug Screening Using Automatic Pipettors

1992 ◽  
Vol 16 (5) ◽  
pp. 340-342 ◽  
Author(s):  
C. Collins ◽  
J. Muto ◽  
V. Spiehler
Keyword(s):  
Drug Screening ◽  
Whole Blood

Comprehensive Drug Screening of Whole Blood by LC–HRMS–MS in a Forensic Laboratory

2020 ◽  
Author(s):  
Jon B Stephenson ◽  
Melanie L Flater ◽  
Joseph Austin ◽  
Lisa T Bain ◽  
Lisa A Holt ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Drug Screening ◽  
Whole Blood ◽  
Drugs Of Abuse ◽  
Screening Method ◽  
Drug Analysis ◽  
Screening Methods ◽  
Blood Samples ◽  
Traditional Drug ◽  
Whole Blood Samples

Abstract As the number of prescriptions, over-the-counter medications and drugs of abuse continue to increase, forensic laboratories are faced with the challenge of developing more comprehensive screening methods in order to detect them in whole blood samples. Another challenge faced by forensic laboratories is detecting and identifying novel synthetic compounds as they emerge and change. Traditional drug screening methods include enzyme immunoassay (EIA) and either gas or liquid chromatography paired with mass spectrometry (GC–MS or LC–MS-MS, respectively). While these methods are good, they have their disadvantages. For example, EIA requires special reagents for each drug class, GC–MS requires extensive sample preparation, and LC–MS-MS only detects drugs on a known inclusion lists of compounds of interest. Described below is the development of a robust and comprehensive screening method for drugs in whole blood samples that eliminates the aforementioned disadvantages of the traditional methods. Using a Q Exactive Focus™ liquid chromatography–high-resolution accurate mass spectrometer (LC–HRMS-MS), a method was developed that is capable of detecting ~200 drugs at a concentration of 2 μg/L for most analytes. This method also employs a more automated data processing feature which reduces processing time. Finally, it has the added benefit of retroactive data analysis, which allows it to be used for unknown drug analysis as well. Used as an initial screening method, the comprehensive drug screen using LC–HRMS-MS has the potential to take on two of the most important challenges faced by forensic laboratories today.

Active Micropump-Mixer for Rapid Antiplatelet Drug Screening in Whole Blood

2019 ◽  
Vol 91 (16) ◽  
pp. 10830-10839 ◽  
Author(s):  
Crispin Szydzik ◽  
Rose J. Brazilek ◽  
Farzan Akbaridoust ◽  
Charitha de Silva ◽  
Mitchell Moon ◽  
...  
Keyword(s):  
Drug Screening ◽  
Whole Blood ◽  
Antiplatelet Drug

Targeted and non-targeted drug screening in whole blood by UHPLC-TOF-MS with data-independent acquisition

2016 ◽  
Vol 9 (7) ◽  
pp. 1052-1061 ◽  
Author(s):  
Christian Brinch Mollerup ◽  
Petur Weihe Dalsgaard ◽  
Marie Mardal ◽  
Kristian Linnet
Keyword(s):  
Drug Screening ◽  
Whole Blood ◽  
Data Independent Acquisition ◽  
Targeted Drug ◽  
Tof Ms

Rapid and simultaneous extraction of acidic and basic drugs from human whole blood for reliable semi-quantitative NAGINATA drug screening by GC–MS

2013 ◽  
Vol 32 (1) ◽  
pp. 97-104 ◽  
Author(s):  
Keiko Kudo ◽  
Yosuke Usumoto ◽  
Kiyotaka Usui ◽  
Makiko Hayashida ◽  
Emiko Kurisaki ◽  
...  
Keyword(s):  
Drug Screening ◽  
Whole Blood ◽  
Basic Drugs ◽  
Simultaneous Extraction ◽  
Human Whole Blood

Fine Structure of Platelet Interaction with a New Microcrystalline Collagen Preparation

1974 ◽  
Vol 32 ◽  
pp. 58-59
Author(s):  
W. H. Zucker ◽  
R. G. Mason
Keyword(s):  
Fine Structure ◽  
Platelet Aggregation ◽  
Hydrochloric Acid ◽  
Whole Blood ◽  
Platelet Adhesion ◽  
Hemostatic Agent ◽  
Native Collagen ◽  
Fibrillar Morphology ◽  
Clinical Interest ◽  
New Form

Platelet adhesion initiates platelet aggregation and is an important component of the hemostatic process. Since the development of a new form of collagen as a topical hemostatic agent is of both basic and clinical interest, an ultrastructural and hematologic study of the interaction of platelets with the microcrystalline collagen preparation was undertaken.In this study, whole blood anticoagulated with EDTA was used in order to inhibit aggregation and permit study of platelet adhesion to collagen as an isolated event. The microcrystalline collagen was prepared from bovine dermal corium; milling was with sharp blades. The preparation consists of partial hydrochloric acid amine collagen salts and retains much of the fibrillar morphology of native collagen.

3-D organization of fibrinogen receptors using computer reconstruction and whole-mount microscopy

1988 ◽  
Vol 46 ◽  
pp. 30-31
Author(s):  
E. T. O'Toole ◽  
R. R. Hantgan ◽  
J. C. Lewis
Keyword(s):  
Whole Blood ◽  
Colloidal Gold ◽  
Blood Platelets ◽  
Cell Contact ◽  
Computer Assisted ◽  
Adherent Cells ◽  
Differential Centrifugation ◽  
Computer Reconstruction ◽  
Sds Page ◽  
Whole Mount

Thrombocytes (TC), the avian equivalent of blood platelets, support hemostasis by aggregating at sites of injury. Studies in our lab suggested that fibrinogen (fib) is a requisite cofactor for TC aggregation but operates by an undefined mechanism. To study the interaction of fib with TC and to identify fib receptors on cells, fib was purified from pigeon plasma, conjugated to colloidal gold and used both to facilitate aggregation and as a receptor probe. Described is the application of computer assisted reconstruction and stereo whole mount microscopy to visualize the 3-D organization of fib receptors at sites of cell contact in TC aggregates and on adherent cells.Pigeon TC were obtained from citrated whole blood by differential centrifugation, washed with Ca++ free Hank's balanced salts containing 0.3% EDTA (pH 6.5) and resuspended in Ca++ free Hank's. Pigeon fib was isolated by precipitation with PEG-1000 and the purity assessed by SDS-PAGE. Fib was conjugated to 25nm colloidal gold by vortexing and the conjugates used as the ligand to identify fib receptors.

Sign in / Sign up

Export Citation Format

Share Document