A novel phase retrieval and reconstruction method in optical diffraction tomography

2019 ◽  
Author(s):  
Shuo Cao ◽  
Xing Lv ◽  
Qiang Chen ◽  
Guanghui Wang ◽  
Jiabi Chen
Keyword(s):  
Phase Retrieval ◽  
Optical Diffraction ◽  
Reconstruction Method ◽  
Diffraction Tomography ◽  
Optical Diffraction Tomography

scholarly journals Reconstruction method for extended depth-of-field optical diffraction tomography

Methods ◽  
2018 ◽  
Vol 136 ◽  
pp. 40-49 ◽  
Author(s):  
Wojciech Krauze ◽  
Arkadiusz Kuś ◽  
Dariusz Śladowski ◽  
Ewa Skrzypek ◽  
Małgorzata Kujawińska
Keyword(s):  
Optical Diffraction ◽  
Depth Of Field ◽  
Reconstruction Method ◽  
Diffraction Tomography ◽  
Optical Diffraction Tomography ◽  
Extended Depth Of Field

Total-shear grating based optical diffraction tomography

2021 ◽  
Author(s):  
Piotr Zdańkowski ◽  
Julianna Winnik ◽  
Paweł Gocłowski ◽  
Maciej Trusiak
Keyword(s):  
Optical Diffraction ◽  
Diffraction Tomography ◽  
Optical Diffraction Tomography ◽  
Total Shear

scholarly journals Mitotic Chromosomes in Live Cells Characterized Using High-Speed and Label-Free Optical Diffraction Tomography

Cells ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 1368 ◽  
Author(s):  
Kim ◽  
Lee ◽  
Fujii ◽  
Lee ◽  
Lee ◽  
...  
Keyword(s):  
Diffusion Coefficient ◽  
Three Dimensional ◽  
Laser Scanning Microscopy ◽  
Optical Diffraction ◽  
Live Cells ◽  
Confocal Laser ◽  
Diffraction Tomography ◽  
Label Free ◽  
Mitotic Chromosomes ◽  
Optical Diffraction Tomography

The cell nucleus is a three-dimensional, dynamic organelle organized into subnuclear compartments such as chromatin and nucleoli. The structure and function of these compartments are maintained by diffusion and interactions between related factors as well as by dynamic and structural changes. Recent studies using fluorescent microscopic techniques suggest that protein factors can access and are freely mobile in heterochromatin and in mitotic chromosomes, despite their densely packed structure. However, the physicochemical properties of the chromosome during cell division are not fully understood. In the present study, characteristic properties such as the refractive index (RI), volume of the mitotic chromosomes, and diffusion coefficient (D) of fluorescent probes inside the chromosome were quantified using an approach combining label-free optical diffraction tomography with complementary confocal laser-scanning microscopy and fluorescence correlation spectroscopy. Variations in these parameters correlated with osmotic conditions, suggesting that changes in RI are consistent with those of the diffusion coefficient for mitotic chromosomes and cytosol. Serial RI tomography images of chromosomes in live cells during mitosis were compared with three-dimensional confocal micrographs to demonstrate that compaction and decompaction of chromosomes induced by osmotic change were characterized by linked changes in chromosome RI, volume, and the mobilities of fluorescent proteins.

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