Correlative 3D anatomy and spatial chemistry in animal-microbe symbioses: developing sample preparation for phase-contrast synchrotron radiation based micro-computed tomography and mass spectrometry imaging

2019 ◽  
Author(s):  
Benedikt Geier ◽  
Maximilian Franke ◽  
Bernhard Ruthensteiner ◽  
Miguel Ángel González Porras ◽  
Alexander Gruhl ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Computed Tomography ◽  
Synchrotron Radiation ◽  
Sample Preparation ◽  
Phase Contrast ◽  
Mass Spectrometry Imaging ◽  
Micro Computed Tomography ◽  
3D Anatomy

Laser Use in Endodontic for Increase the Adhesion of Root Canal Filling. A synchrotron radiation micro tomography study

2018 ◽  
Vol 69 (8) ◽  
pp. 2144-2149
Author(s):  
Carmen Todea ◽  
Daliana Mocuta ◽  
Adrian Manescu ◽  
Gianfranco Semez ◽  
Alessandra Giuliani ◽  
...  
Keyword(s):  
Computed Tomography ◽  
High Resolution ◽  
Synchrotron Radiation ◽  
Root Canal ◽  
Phase Contrast ◽  
Mechanical Treatment ◽  
Apical Region ◽  
Micro Computed Tomography ◽  
Root Canal Filling ◽  
Apical Area

The aim of the present paper is to explore, through a high-resolution synchrotron radiation-based micro computed tomography experiment, the efficiency of the Er:YAG laser involvement in the debridement of the root canal of pathologic teeth, applied after the chemo-mechanical treatment of the canal. The adhesion quality of root canal filling is the consequence of the root canal cleaning and shaping, thus preventing the leakage. Twelve extracted single-rooted teeth were considered. The first 5 mm of the roots, corresponding to the apical region of the teeth, were initially investigated through high-resolution synchrotron radiation phase-contrast micro computed tomography. Afterwards, a chemo-mechanical endodontic treatment was performed and the same region of the teeth was imaged again. Finally, an Er:YAG laser with PIPS tip treatment was applied on the same teeth and a last tomographic scan was done. The chemo-mechanical treatment properly removed the necrotic tissue only in the upper part of the apical area of root canal, but tissue debris were found in the lower and partially also in the medium and upper part of the apical region, especially when the apical area exhibited a larger curvature. The laser treatment applied afterwards gave good results in terms of removing the debris from the apical area of root canal, mainly when they remained in the main canal. The present study proves the capability of Er:YAG with PIPS tips laser treatment to enhance the removing of tissue debris from the apical region of root canals after a chemo-mechanical treatment. The high-resolution synchrotron radiation-based phase contrast micro computed tomography is the most powerful tool for a non-destructive 3D investigation method of the root canal morphology analysis and a proper characterization technique for the assessment of the endodontic treatment.

Cortical and trabecular bone parameters from HR-Pqct images at the Tibia: a local comparison with synchrotron radiation micro-computed tomography

2013 ◽  
Author(s):  
Agnes Ostertag ◽  
Francoise Peyrin ◽  
Sylvie Fernandez ◽  
Jean-Denis Laredo ◽  
Vernejoul Marie-Christine De ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Synchrotron Radiation ◽  
Trabecular Bone ◽  
Micro Computed Tomography ◽  
Bone Parameters ◽  
Local Comparison

scholarly journals Sample preparation strategy for the detection of steroid-like compounds using MALDI mass spectrometry imaging: pulmonary distribution of budesonide as a case study

2021 ◽  
Author(s):  
Riccardo Zecchi ◽  
Pietro Franceschi ◽  
Laura Tigli ◽  
Davide Amidani ◽  
Chiara Catozzi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Mass Spectrometry Imaging ◽  
Maldi Mass Spectrometry ◽  
Adult Rabbit ◽  
Chronic Obstructive ◽  
Ionization Mass ◽  
Obstructive Pulmonary Disease ◽  
Imaging Protocol ◽  
Girard’S Reagent

AbstractCorticosteroids as budesonide can be effective in reducing topic inflammation processes in different organs. Therapeutic use of budesonide in respiratory diseases, like asthma, chronic obstructive pulmonary disease, and allergic rhinitis is well known. However, the pulmonary distribution of budesonide is not well understood, mainly due to the difficulties in tracing the molecule in lung samples without the addition of a label. In this paper, we present a matrix-assisted laser desorption/ionization mass spectrometry imaging protocol that can be used to visualize the pulmonary distribution of budesonide administered to a surfactant-depleted adult rabbit. Considering that budesonide is not easily ionized by MALDI, we developed an on-tissue derivatization method with Girard’s reagent P followed by ferulic acid deposition as MALDI matrix. Interestingly, this sample preparation protocol results as a very effective strategy to raise the sensitivity towards not only budesonide but also other corticosteroids, allowing us to track its distribution and quantify the drug inside lung samples. Graphical abstract

The effect of risedronate on bone mineralization as measured by micro-computed tomography with synchrotron radiation: Correlation to histomorphometric indices of turnover

Bone ◽  
2005 ◽  
Vol 37 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Babul Borah ◽  
Erik L. Ritman ◽  
Thomas E. Dufresne ◽  
Steven M. Jorgensen ◽  
Sheng Liu ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Synchrotron Radiation ◽  
Bone Mineralization ◽  
Micro Computed Tomography
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