Substrate developments for exploring living cells in culture with quantitative phase imaging: towards label-free high-content screening (Conference Presentation)

2019 ◽  
Author(s):  
Pierre P. Marquet ◽  
Erik Bélanger ◽  
Bertrand de-Dorlodot ◽  
Émile Rioux-Pélerin ◽  
Sébastien Lévesque
Keyword(s):  
Living Cells ◽  
High Content Screening ◽  
Phase Imaging ◽  
Label Free ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase ◽  
Cells In Culture

Multi-modal and photothermal quantitative phase imaging of living cells with nanoparticles in a combined setup

2017 ◽  
Author(s):  
Björn Kemper ◽  
Nir A. Turko ◽  
Michael Isbach ◽  
Steffi Ketelhut ◽  
Burkhard Greve ◽  
...  
Keyword(s):  
Living Cells ◽  
Phase Imaging ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase

scholarly journals Reagent- and actuator-free analysis of individual erythrocytes using three-dimensional quantitative phase imaging and capillary microfluidics

2021 ◽  
Author(s):  
DongHun Ryu ◽  
Hyeono Nam ◽  
Jessie Sungyun Jeon ◽  
YongKeun Park
Keyword(s):  
Blood Cells ◽  
Histopathological Examination ◽  
Point Of Care ◽  
Hemoglobin Concentration ◽  
Biochemical Properties ◽  
Phase Imaging ◽  
Sample Treatment ◽  
Label Free ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase

Histopathological examination of blood cells plays a crucial role in the diagnosis of various diseases. However, it involves time-consuming and laborious staining procedures required for microscopic review by medical experts and is not directly applicable for point-of-care diagnosis in resource-limited locations. This study reports a dilution-, actuation- and label-free method for the analysis of individual red blood cells (RBCs) using a capillary microfluidic device and quantitative phase imaging. Blood, without any sample treatment, is directly loaded into a micrometer-thick channel such that it forms a quasi-monolayer inside the channel. The morphological and biochemical properties of RBCs, including hemoglobin concentration, hemoglobin content, and corpuscular volume, were retrieved using the refractive index tomograms of individual RBCs measured using 3D quantitative phase imaging. The deformability of individual RBCs was also obtained by measuring the dynamic membrane fluctuations. The proposed framework applies to other imaging modalities and biomedical applications, facilitating rapid and cost-effective diagnosis and prognosis of diseases.

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