New staining tools and developments for 3D soft tissue CT imaging

2019 ◽  
Author(s):  
Madleen Busse ◽  
Simone Ferstl ◽  
Mark Müller ◽  
Melanie A. Kimm ◽  
Enken Drecoll ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Ct Imaging

scholarly journals Micro-CT imaging of Thiel-embalmed and iodine-stained human temporal bone for 3D modeling

2021 ◽  
Vol 50 (1) ◽  
Author(s):  
Sebastian Halm ◽  
David Haberthür ◽  
Elisabeth Eppler ◽  
Valentin Djonov ◽  
Andreas Arnold
Keyword(s):  
Soft Tissue ◽  
Temporal Bone ◽  
Middle Ear ◽  
3D Modeling ◽  
Soft Tissues ◽  
Ct Imaging ◽  
Micro Ct ◽  
Data Set ◽  
Iodine Staining ◽  
Tissue Structures

Abstract Introduction This pilot study explores whether a human Thiel-embalmed temporal bone is suitable for generating an accurate and complete data set with micro-computed tomography (micro-CT) and whether solid iodine-staining improves visualization and facilitates segmentation of middle ear structures. Methods A temporal bone was used to verify the accuracy of the imaging by first digitally measuring the stapes on the tomography images and then physically under the microscope after removal from the temporal bone. All measurements were compared with literature values. The contralateral temporal bone was used to evaluate segmentation and three-dimensional (3D) modeling after iodine staining and micro-CT scanning. Results The digital and physical stapes measurements differed by 0.01–0.17 mm or 1–19%, respectively, but correlated well with the literature values. Soft tissue structures were visible in the unstained scan. However, iodine staining increased the contrast-to-noise ratio by a factor of 3.7 on average. The 3D model depicts all ossicles and soft tissue structures in detail, including the chorda tympani, which was not visible in the unstained scan. Conclusions Micro-CT imaging of a Thiel-embalmed temporal bone accurately represented the entire anatomy. Iodine staining considerably increased the contrast of soft tissues, simplified segmentation and enabled detailed 3D modeling of the middle ear.

WE-D-I-6B-01: Soft Tissue Visualization Using a Highly Efficient Megavoltage Cone Beam CT Imaging System

2005 ◽  
Vol 32 (6Part19) ◽  
pp. 2131-2131 ◽  
Author(s):  
F Ghelmansarai ◽  
A Bani-Hashemi ◽  
J Pouliot ◽  
E Calderon ◽  
P Hernandez ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Imaging System ◽  
Cone Beam Ct ◽  
Ct Imaging ◽  
Cone Beam ◽  
Highly Efficient ◽  
Megavoltage Cone Beam Ct

scholarly journals Feasibility of dual phase 99mTc-MDP SPECT/CT imaging in rheumatoid arthritis evaluation

2020 ◽  
Author(s):  
Yasser G. Abdelhafez ◽  
Felipe Godinez ◽  
Kanika Sood ◽  
Rosalie J. Hagge ◽  
Robert D. Boutin ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Soft Tissue ◽  
Intraclass Correlation ◽  
Blood Pool ◽  
Ct Imaging ◽  
Observer Agreement ◽  
Dual Phase ◽  
Imaging Protocol ◽  
Radiotracer Injection ◽  
Tissue Phase

AbstractObjectivesTo prospectively demonstrate the feasibility of performing dual-phase SPECT/CT for the assessment of the small joints of the hands of rheumatoid arthritis (RA) patients, and to evaluate the reliability of the quantitative and qualitative measures derived from the resulting images.MethodsA SPECT/CT imaging protocol was developed in this pilot study to scan both hands simultaneously in RA patients, in two phases of 99mTc-MDP radiotracer uptake; namely the soft-tissue blood pool phase (within 15 minutes after radiotracer injection) and osseous phase (after 3 hours). Joints were evaluated qualitatively (normal vs. abnormal uptake) and quantitatively (by measuring the maximum corrected count ratio [MCCR]). Qualitative and quantitative evaluations were repeated to assess reliability.ResultsFour participants completed seven studies (all four were imaged at baseline, and three of them at follow-up after 1-month of arthritis therapy). A total of 280 joints (20 per hand) were evaluated. The MCCR from soft-tissue phase scans was significantly higher for clinically abnormal joints compared to clinically normal ones; p<0.001, however the MCCR from the osseous phase scans were not different between the two groups. Intraclass Correlation Coefficient (ICC) for MCCR was excellent (0.9789, 95% confidence interval [CI]: 0.9734-0.9833). Intra-observer agreement for qualitative SPECT findings was good for both the soft-tissue phase (kappa=0.78, 95%CI: 0.72-0.83) and osseous-phase (kappa=0.70, 95%CI: 0.64-0.76) scans.ConclusionExtracting reliable quantitative and qualitative measures from dual-phase 99mTc-MDP SPECT/CT hand scans is feasible in RA patients. SPECT/CT may provide a unique means for assessing both synovitis and osseous involvement in RA joints using the same radiotracer injection.

scholarly journals Differentiation of soft tissue and bone sarcomas from benign lesions utilizing 18F- FDG PET/CT-derived parameters

2020 ◽  
Author(s):  
Bo Chen ◽  
Hongbo Feng ◽  
Jinghui Xie ◽  
Chun Li ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Risk Factors ◽  
Soft Tissue ◽  
Regression Model ◽  
Fdg Pet ◽  
Univariate Analysis ◽  
Ct Imaging ◽  
Bone Lesions ◽  
Benign Lesions ◽  
Pet Ct ◽  
Fdg Pet Ct

Abstract Background: Accurate differentiation between malignant and benign in soft tissue and bone lesions is essential for the prevention of excessive pathological biopsy and unplanned surgical resection. However, it remains a challenge and a standard diagnosis modality is urgently needed. The purpose of this study is to evaluate the usefulness of 18F- FDG PET/CT-derived parameters to differentiate soft tissue sarcoma (STS) and bone sarcoma (BS) from benign lesions.Methods: Patients who underwent pretreatment 18F-FDG PET/CT imaging and subsequent biopsy to confirm malignant (STS and BS, n=37) and benign (n=33) soft tissue and bone lesions were retrospectively reviewed. The tumor size, maximum standardized uptake value (SUVmax), metabolic tumor volume (MTV), total lesion glycolysis (TLG) and heterogeneous factor (HF) of each lesion were measured. Univariate analysis and multivariate logistic regression analysis were performed to screen the significant risk factors to distinguish STS and BS from benign lesions. To establish a regression model based on independent risk factors, receiver operating characteristic (ROC) analysis was performed to assess the diagnostic performances.Results: Univariate analysis results showed that tumor size, SUVmax, MTV, TLG and HF of 18F-FDG PET/CT imaging in STS and BS group were all higher than benign lesions group, the difference were statistically significant (all P value were<0.01). However, the multivariate regression model only included SUVmax and HF as independent risk factors, odds radios were 1.135(95%CI: 1.026~1.256, P =0.014), 7.869(95%CI: 2.119~29.230, P =0.002), respectively. The regression model was as follow: Logit (P) = -2.461+0.127SUVmax+2.063HF. The area under the ROC was 0.860 (95%CI: 0.771~0.948, P <0.001) higher than SUVmax 0.744(95%CI: 0.628~0.860, P <0.001) and HF 0.790 (95%CI:0.684~0.896, P <0.001).Conclusion: The regression model including SUVmax and HF based on 18F-FDG PET/CT imaging may be useful for differentiating STS and BS from benign lesions.

scholarly journals FDG-PET/CT Imaging Predicts Histopathologic Treatment Responses after the Initial Cycle of Neoadjuvant Chemotherapy in High-Grade Soft-Tissue Sarcomas

2009 ◽  
Vol 15 (8) ◽  
pp. 2856-2863 ◽  
Author(s):  
Matthias R. Benz ◽  
Johannes Czernin ◽  
Martin S. Allen-Auerbach ◽  
William D. Tap ◽  
Sarah M. Dry ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Neoadjuvant Chemotherapy ◽  
Fdg Pet ◽  
Soft Tissue Sarcomas ◽  
Ct Imaging ◽  
High Grade ◽  
Pet Ct ◽  
Treatment Responses ◽  
Fdg Pet Ct ◽  
Initial Cycle

Functional CT imaging of angiogenesis in rabbit VX2 soft-tissue tumour

2001 ◽  
Vol 46 (12) ◽  
pp. 3161-3175 ◽  
Author(s):  
Thomas G Purdie ◽  
Elizabeth Henderson ◽  
Ting-Yim Lee
Keyword(s):  
Soft Tissue ◽  
Ct Imaging ◽  
Soft Tissue Tumour ◽  
Tissue Tumour ◽  
Functional Ct

scholarly journals Volume-Based F-18 FDG PET/CT Imaging Markers Provide Supplemental Prognostic Information to Histologic Grading in Patients With High-Grade Bone or Soft Tissue Sarcoma

Medicine ◽  
2015 ◽  
Vol 94 (51) ◽  
pp. e2319 ◽  
Author(s):  
Kim Francis Andersen ◽  
Hanna Maria Fuglo ◽  
Sine Hvid Rasmussen ◽  
Michael Mork Petersen ◽  
Annika Loft
Keyword(s):  
Soft Tissue ◽  
Soft Tissue Sarcoma ◽  
Fdg Pet ◽  
Ct Imaging ◽  
High Grade ◽  
Prognostic Information ◽  
Histologic Grading ◽  
Pet Ct ◽  
Imaging Markers ◽  
Fdg Pet Ct

scholarly journals Soft-tissue Sarcoma Metastases Identified on Abdomen and Pelvis CT Imaging

2009 ◽  
Vol 467 (11) ◽  
pp. 2838-2844 ◽  
Author(s):  
David M. King ◽  
Donald A. Hackbarth ◽  
Chris M. Kilian ◽  
Guillermo F. Carrera
Keyword(s):  
Soft Tissue ◽  
Soft Tissue Sarcoma ◽  
Ct Imaging

scholarly journals MICRO-CT Imaging of Thiel-Embalmed and Iodine-Stained Human Temporal Bone for 3D Modeling

2020 ◽  
Author(s):  
Halm Sebastian ◽  
Haberthuer David ◽  
Eppler Elisabeth ◽  
Djonov Valentin ◽  
Arnold Andreas
Keyword(s):  
Soft Tissue ◽  
Temporal Bone ◽  
Middle Ear ◽  
3D Modeling ◽  
Ct Imaging ◽  
Micro Ct ◽  
Data Set ◽  
Iodine Staining ◽  
Tissue Structures ◽  
Temporal Bones

ABSTRACT OBJECTIVE The following study investigates whether human Thiel-embalmed temporal bones are suitable for generating an accurate and complete data set with micro-computed tomography (micro-CT) and the effect of solid iodine staining for improved visualization and facilitated segmentation of middle ear structures. METHODS One temporal bone was used to verify the accuracy of the imaging by measuring the stapes digitally on the tomography images and physically under the microscope after removal from the temporal bone. All measurements were compared with literature values. The contralateral temporal bone was used to evaluate segmentation and 3D modeling after iodine staining and micro-CT scanning. RESULTS The digital and physical stapes measurements differ up to 0.17 mm and 24% but correlate well with the literature values. Soft tissue structures were visible in the unstained scan. However, iodine staining increased the contrast-to-noise ratio by a factor 3.7 on average. The 3D model depicts all ossicles and soft tissue structures in detail, including the chorda tympani, which was not visible in the unstained scan. CONCLUSION Micro-CT imaging of Thiel-embalmed temporal bones accurately represents the entire anatomy. Iodine staining considerably increases the contrast of soft tissues, simplifies segmentation and enables detailed 3D modeling of the middle ear, which is suitable for further use as a finite element model (FEM).

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