scholarly journals Combined multi-wavelength laser speckle contrast imaging and diffuse reflectance imaging for skin perfusion assessment

2019 ◽  
Author(s):  
Anton Y. Sdobnov ◽  
Alexander Bykov ◽  
Alexey Popov ◽  
Ilze Lihacova ◽  
Alexey Lihachev ◽  
...  
Keyword(s):  
Diffuse Reflectance ◽  
Laser Speckle ◽  
Contrast Imaging ◽  
Skin Perfusion ◽  
Laser Speckle Contrast Imaging ◽  
Speckle Contrast ◽  
Multi Wavelength ◽  
Perfusion Assessment ◽  
Diffuse Reflectance Imaging

Laser Speckle Contrast Imaging for Intraoperative Quantitative Assessment of Intestinal Blood Perfusion During Colorectal Surgery: A Prospective Pilot Study

2019 ◽  
Vol 26 (3) ◽  
pp. 293-301 ◽  
Author(s):  
Shigehiro Kojima ◽  
Tsuguo Sakamoto ◽  
Yuko Nagai ◽  
Yutaka Matsui ◽  
Kyojiro Nambu ◽  
...  
Keyword(s):  
Pilot Study ◽  
Colorectal Surgery ◽  
Intraclass Correlation ◽  
Laser Speckle ◽  
Fluorescence Angiography ◽  
Contrast Imaging ◽  
Flow Measurements ◽  
Laser Speckle Contrast Imaging ◽  
Speckle Contrast ◽  
Perfusion Assessment

Background. The main limitation of perfusion assessment with indocyanine green fluorescence angiography during colorectal surgery is that the surgeon assesses the quality of perfusion subjectively. The ideal intestinal viability test must be minimally invasive, objective, and reproducible. We evaluated the quantitativity and reproducibility of laser speckle contrast imaging for perfusion assessment during colorectal surgery. Methods. This was a prospective, nonrandomized, pilot study of 8 consecutive patients who underwent elective left-sided colorectal resection. Laser speckle perfusion images at the site of proximal transection of the bowel were obtained intraoperatively. We tested the hypothesis that laser speckle contrast imaging was able to quantitatively identify areas of diminished intestinal perfusion after devascularization and assessed the reproducibility of this method. Results. All surgical procedures were uneventful and blood flow measurements were successfully made in all patients. None of the patients developed postoperative complications related to the anastomosis and stoma. Data analyses were successfully optimized to perform quantitative regional perfusion assessments in all cases. The bowel tissue blood flows of the anal side region adjacent to the transection line were significantly lower than those of the oral side region adjacent to the transection line after ligation of marginal vessels ( P = .012). Interrater reliability was high (intraclass correlation coefficients = 0.989), and a Bland-Altman plot showed few differences of mean flux data between 2 investigators. Conclusion. Laser speckle contrast imaging is feasible for real-time assessment of bowel perfusion with quantitativity and excellent reproducibility during colorectal surgery without administration of any contrast agents.

Laser speckle contrast imaging and quantitative fluorescence angiography for perfusion assessment

2019 ◽  
Vol 404 (4) ◽  
pp. 505-515 ◽  
Author(s):  
Jonas Hedelund Rønn ◽  
Nikolaj Nerup ◽  
Rune Broni Strandby ◽  
Morten Bo Søndergaard Svendsen ◽  
Rikard Ambrus ◽  
...  
Keyword(s):  
Laser Speckle ◽  
Fluorescence Angiography ◽  
Contrast Imaging ◽  
Laser Speckle Contrast Imaging ◽  
Speckle Contrast ◽  
Perfusion Assessment ◽  
Quantitative Fluorescence

scholarly journals Nephron blood flow dynamics measured by laser speckle contrast imaging

2011 ◽  
Vol 300 (2) ◽  
pp. F319-F329 ◽  
Author(s):  
Niels-Henrik Holstein-Rathlou ◽  
Olga V. Sosnovtseva ◽  
Alexey N. Pavlov ◽  
William A. Cupples ◽  
Charlotte Mehlin Sorensen ◽  
...  
Keyword(s):  
Blood Flow ◽  
Signal Transmission ◽  
Experimental Studies ◽  
Flow Dynamics ◽  
Laser Speckle ◽  
Tubuloglomerular Feedback ◽  
Contrast Imaging ◽  
Laser Speckle Contrast Imaging ◽  
Speckle Contrast ◽  
Blood Flow Dynamics

Tubuloglomerular feedback (TGF) has an important role in autoregulation of renal blood flow and glomerular filtration rate (GFR). Because of the characteristics of signal transmission in the feedback loop, the TGF undergoes self-sustained oscillations in single-nephron blood flow, GFR, and tubular pressure and flow. Nephrons interact by exchanging electrical signals conducted electrotonically through cells of the vascular wall, leading to synchronization of the TGF-mediated oscillations. Experimental studies of these interactions have been limited to observations on two or at most three nephrons simultaneously. The interacting nephron fields are likely to be more extensive. We have turned to laser speckle contrast imaging to measure the blood flow dynamics of 50–100 nephrons simultaneously on the renal surface of anesthetized rats. We report the application of this method and describe analytic techniques for extracting the desired data and for examining them for evidence of nephron synchronization. Synchronized TGF oscillations were detected in pairs or triplets of nephrons. The amplitude and the frequency of the oscillations changed with time, as did the patterns of synchronization. Synchronization may take place among nephrons not immediately adjacent on the surface of the kidney.

scholarly journals Laser speckle contrast imaging for perfusion monitoring during surgical reconstruction of anorectal malformations

2021 ◽  
Vol 66 ◽  
pp. 101793
Author(s):  
Pernilla Stenström ◽  
Rafi Sheikh ◽  
Kristine Hagelsteen ◽  
Johanna Wennström Berggren ◽  
Malin Malmsjö
Keyword(s):  
Anorectal Malformations ◽  
Laser Speckle ◽  
Surgical Reconstruction ◽  
Contrast Imaging ◽  
Laser Speckle Contrast Imaging ◽  
Speckle Contrast

scholarly journals Monitoring microvascular perfusion variations with laser speckle contrast imaging using a view-based temporal template method

2017 ◽  
Vol 111 ◽  
pp. 49-59 ◽  
Author(s):  
Mohammad Zaheer Ansari ◽  
Eun-Jeung Kang ◽  
Mioara D. Manole ◽  
Jens P. Dreier ◽  
Anne Humeau-Heurtier
Keyword(s):  
Laser Speckle ◽  
Microvascular Perfusion ◽  
Template Method ◽  
Contrast Imaging ◽  
Laser Speckle Contrast Imaging ◽  
Speckle Contrast

Enhancing vascular visualization in laser speckle contrast imaging of blood flow using multi-focus image fusion

2018 ◽  
Vol 12 (1) ◽  
pp. e201800100 ◽  
Author(s):  
Wenzhi Lv ◽  
Yang Wang ◽  
Xiao Chen ◽  
Xiaoxi Fu ◽  
Jinling Lu ◽  
...  
Keyword(s):  
Blood Flow ◽  
Image Fusion ◽  
Laser Speckle ◽  
Contrast Imaging ◽  
Laser Speckle Contrast Imaging ◽  
Speckle Contrast ◽  
Focus Image
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