Improved and fast biotissue imaging by temporal focusing widefield multiphoton microscopy with spatially modulated illumination

2019 ◽  
Author(s):  
Chia-Yuan Chang ◽  
Chun-Yu Lin ◽  
Vladimir Hovhannisyan ◽  
Shean-Jen Chen
Keyword(s):  
Multiphoton Microscopy ◽  
Temporal Focusing

scholarly journals Dynamic particle tracking via temporal focusing multiphoton microscopy with astigmatism imaging

2014 ◽  
Vol 22 (22) ◽  
pp. 27290 ◽  
Author(s):  
Chi-Hsiang Lien ◽  
Chun-Yu Lin ◽  
Shean-Jen Chen ◽  
Fan-Ching Chien
Keyword(s):  
Particle Tracking ◽  
Multiphoton Microscopy ◽  
Temporal Focusing

Temporal focusing-based widefield multiphoton microscopy with spatially modulated illumination for biotissue imaging

2017 ◽  
Vol 11 (1) ◽  
pp. e201600287 ◽  
Author(s):  
Chia-Yuan Chang ◽  
Cheng-Han Lin ◽  
Chun-Yu Lin ◽  
Yong-Da Sie ◽  
Yvonne Yuling Hu ◽  
...  
Keyword(s):  
Multiphoton Microscopy ◽  
Temporal Focusing

scholarly journals Fast volumetric imaging with patterned illumination via digital micro-mirror device-based temporal focusing multiphoton microscopy

2016 ◽  
Vol 7 (5) ◽  
pp. 1727 ◽  
Author(s):  
Chia-Yuan Chang ◽  
Yvonne Yuling Hu ◽  
Chun-Yu Lin ◽  
Cheng-Han Lin ◽  
Hsin-Yu Chang ◽  
...  
Keyword(s):  
Multiphoton Microscopy ◽  
Volumetric Imaging ◽  
Temporal Focusing ◽  
Patterned Illumination

scholarly journals Overcoming tissue scattering in wide-field deep imaging by extended detection and computational reconstruction

2019 ◽  
Author(s):  
Yuanlong Zhang ◽  
Tiankuang Zhou ◽  
Xuemei Hu ◽  
Hao Xie ◽  
Lu Fang ◽  
...  
Keyword(s):  
Multiphoton Microscopy ◽  
Tissue Imaging ◽  
Wide Field ◽  
Temporal Focusing ◽  
Tissue Scattering ◽  
Deep Imaging ◽  
Detection Mode ◽  
Computational Reconstruction ◽  
Imaging Depth

AbstractCompared to the golden technique of point‐scanning multiphoton microscopy, line‐scanning temporal focusing microscopy (LTFM) is competitive in high imaging speed while maintaining tight axial confinement. However, considering its wide‐field detection mode, LTFM suffers from shallow penetration depth as a result of crosstalk induced by tissue scattering. In contrast to the spatial filtering based on confocal slit detection, we propose the extended detection LTFM (ED‐LTFM), the first technique to extract signals from scattered photons and thus effectively extend the imaging depth. By recording a succession of line‐shape excited signals in 2D and reconstructing signals under Hessian regularization, we can push the depth limitation in scattering tissue imaging. We valid the concept with numerical simulations, and demonstrate the performance of enhanced imaging depth in in vivo imaging of mouse brains.

scholarly journals Increasing the penetration depth of temporal focusing multiphoton microscopy for neurobiological applications

2019 ◽  
Vol 52 (26) ◽  
pp. 264001 ◽  
Author(s):  
Christopher J Rowlands ◽  
Oliver T Bruns ◽  
Daniel Franke ◽  
Dai Fukamura ◽  
Rakesh K Jain ◽  
...  
Keyword(s):  
Penetration Depth ◽  
Multiphoton Microscopy ◽  
Temporal Focusing

scholarly journals Wavefront sensorless adaptive optics temporal focusing-based multiphoton microscopy

2014 ◽  
Vol 5 (6) ◽  
pp. 1768 ◽  
Author(s):  
Chia-Yuan Chang ◽  
Li-Chung Cheng ◽  
Hung-Wei Su ◽  
Yvonne Yuling Hu ◽  
Keng-Chi Cho ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Multiphoton Microscopy ◽  
Temporal Focusing

2D simultaneous spatial and temporal focusing multiphoton microscopy for fast volume imaging with improved sectioning ability

2015 ◽  
Author(s):  
Qiyuan Song ◽  
Keisuke Isobe ◽  
Kenichi Hirosawa ◽  
Katsumi Midorikawa ◽  
Fumihiko Kannari
Keyword(s):  
Multiphoton Microscopy ◽  
Volume Imaging ◽  
Temporal Focusing
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