Response of patient-specific cell cultures to photodynamic treatment analyzed by digital holographic microscopy

2019 ◽  
Author(s):  
Anna Zhikhoreva ◽  
Andrey Belashov ◽  
Natalya Avdonkina ◽  
Irina Baldueva ◽  
Anna Danilova ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Digital Holographic Microscopy ◽  
Patient Specific ◽  
Specific Cell ◽  
Photodynamic Treatment ◽  
Holographic Microscopy

Morphological Changes in Malignant Tumor Cells at Photodynamic Treatment Assessed by Digital Holographic Microscopy

2019 ◽  
Vol 13 (3) ◽  
pp. 394-400 ◽  
Author(s):  
A. A. Zhikhoreva ◽  
A. V. Belashov ◽  
D. A. Gorbenko ◽  
N. A. Avdonkina ◽  
I. A. Baldueva ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Malignant Tumor ◽  
Morphological Changes ◽  
Digital Holographic Microscopy ◽  
Photodynamic Treatment ◽  
Holographic Microscopy

scholarly journals Quantitative assessment of changes in cellular morphology at photodynamic treatment in vitro by means of digital holographic microscopy

2019 ◽  
Vol 10 (10) ◽  
pp. 4975 ◽  
Author(s):  
A. V. Belashov ◽  
A. A. Zhikhoreva ◽  
T. N. Belyaeva ◽  
N. N. Nikolsky ◽  
I. V. Semenova ◽  
...  
Keyword(s):  
Quantitative Assessment ◽  
Cellular Morphology ◽  
Digital Holographic Microscopy ◽  
Photodynamic Treatment ◽  
Holographic Microscopy

Digital holographic microscopy in label-free analysis of cultured cells’ response to photodynamic treatment

2016 ◽  
Vol 41 (21) ◽  
pp. 5035 ◽  
Author(s):  
A. V. Belashov ◽  
A. A. Zhikhoreva ◽  
T. N. Belyaeva ◽  
E. S. Kornilova ◽  
N. V. Petrov ◽  
...  
Keyword(s):  
Cultured Cells ◽  
Digital Holographic Microscopy ◽  
Label Free ◽  
Photodynamic Treatment ◽  
Holographic Microscopy

scholarly journals An engineered fluidic device to achieve multiplexed monitoring of cell cultures with digital holographic microscopy

2021 ◽  
Author(s):  
Erik Bélanger ◽  
Carine Ben Adiba ◽  
Émile Rioux-Pellerin ◽  
Frédéric Becq ◽  
Pascal Jourdain ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Digital Holographic Microscopy ◽  
Fluidic Device ◽  
Holographic Microscopy

Biophysical monitoring of cell cultures for quality assessment utilizing digital holographic microscopy

2017 ◽  
Author(s):  
Lena Kastl ◽  
Michael Isbach ◽  
Dieter Dirksen ◽  
Jürgen Schnekenburger ◽  
Björn Kemper
Keyword(s):  
Quality Assessment ◽  
Cell Cultures ◽  
Digital Holographic Microscopy ◽  
Holographic Microscopy

scholarly journals Cardiomyocyte-Specific Circulating Cell-Free Methylated DNA in Esophageal Cancer Patients Treated with Chemoradiation

2021 ◽  
Vol 3 (3) ◽  
pp. 100-112
Author(s):  
Sarah Martinez Roth ◽  
Eveline E. Vietsch ◽  
Megan E. Barefoot ◽  
Marcel O. Schmidt ◽  
Matthew D. Park ◽  
...  
Keyword(s):  
Esophageal Cancer ◽  
Cardiac Toxicity ◽  
Amplicon Sequencing ◽  
Neoadjuvant Chemoradiation ◽  
Patient Specific ◽  
High Dose ◽  
Specific Cell ◽  
Methylated Dna ◽  
Bisulfite Treatment ◽  
Dose Radiation

Thoracic high-dose radiation therapy (RT) for cancer has been associated with early and late cardiac toxicity. To assess altered rates of cardiomyocyte cell death due to RT we monitored changes in cardiomyocyte-specific, cell-free methylated DNA (cfDNA) shed into the circulation. Eleven patients with distal esophageal cancer treated with neoadjuvant chemoradiation to 50.4 Gy (RT) and concurrent carboplatin and paclitaxel were enrolled. Subjects underwent fasting blood draws prior to the initiation and after completion of RT as well as 4–6 months following RT. An island of six unmethylated CpGs in the FAM101A locus was used to identify cardiomyocyte-specific cfDNA in serum. After bisulfite treatment this specific cfDNA was quantified by amplicon sequencing at a depth of >35,000 reads/molecule. Cardiomyocyte-specific cfDNA was detectable before RT in the majority of patient samples and showed some distinct changes during the course of treatment and recovery. We propose that patient-specific cardiac damages in response to the treatment are indicated by these changes although co-morbidities may obscure treatment-specific events.

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