Digital holographic microscopy for thickness characterization using synthetized partially coherent holograms

2019 ◽  
Author(s):  
Marta Mikula ◽  
Juan Martínez-Carranza ◽  
Tomasz Kozacki
Keyword(s):  
Digital Holographic Microscopy ◽  
Partially Coherent ◽  
Holographic Microscopy

scholarly journals Partially Coherent Illumination Based Point-Diffraction Digital Holographic Microscopy Study Dynamics of Live Cells

2021 ◽  
Vol 9 ◽  
Author(s):  
Kequn Zhuo ◽  
Yu Wang ◽  
Yang Wang ◽  
Kai Wen ◽  
Min Liu ◽  
...  
Keyword(s):  
Speckle Noise ◽  
Microscopy Study ◽  
Digital Holographic Microscopy ◽  
Live Cells ◽  
Dynamic Processes ◽  
Label Free ◽  
Fringe Contrast ◽  
Partially Coherent ◽  
Holographic Microscopy ◽  
Coherent Illumination

This paper presents a partially coherent point-diffraction digital holographic microscopy (PC-pDHM) prototype and demonstrates its application in label-free imaging of the dynamic processes of live cells. In PC-pDHM, the light scattered by a rotating diffuser is coupled into a multi-mode fiber, and the output light is used as the partially coherent illumination (PCI), which reduces the speckle noise significantly in PC-pDHM. A polarization-grating is used to remold the object and the reference waves, and the fringe contrast of the generated hologram can be adjusted by changing the polarization of the illumination wave. Using the PC-pDHM prototype, transparent samples and notably the dynamic processes of live cells were imaged with high contrast and in a label-free manner, discovering the pathological mechanisms of biology in the cellular and sub-cellular levels.

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