The use of theoretical and experimental methods of Raman spectroscopy in the determination of unsaturated fatty acids in vegetable oils

2019 ◽  
Author(s):  
Kirill V. Berezin ◽  
Konstantin N. Dvoretsky ◽  
Maria L. Chernavina ◽  
Anna V. Novoselova ◽  
Vladimir V. Nechaev ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Raman Spectroscopy ◽  
Vegetable Oils ◽  
Unsaturated Fatty Acids ◽  
Experimental Methods

Rapid Determination of Unsaturated Fatty Acids in Vegetable Oil by Raman Spectroscopy and Chemometrics

2016 ◽  
Vol 49 (6) ◽  
pp. 831-842 ◽  
Author(s):  
Jun Bin ◽  
Fangfang Ai ◽  
Wei Fan ◽  
Jiheng Zhou ◽  
Zhimin Zhang
Keyword(s):  
Fatty Acids ◽  
Raman Spectroscopy ◽  
Vegetable Oil ◽  
Unsaturated Fatty Acids ◽  
Rapid Determination

scholarly journals Determination of trans-isomers of unsaturated Fatty acids in foods by FTIR

2020 ◽  
pp. 49-53
Author(s):  
Erdenechimeg N ◽  
Odonchimeg M ◽  
Oyundari M ◽  
Bayarmaa B
Keyword(s):  
Fatty Acids ◽  
Vegetable Oils ◽  
Trans Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Frequency Range ◽  
Trans Isomers ◽  
Negative Effects

It has been proven that the trans-isomers of unsaturated fatty acids that are formed during the hydrogenation of vegetable oils have negative effects on human health. Therefore, it is important to control the content of trans fatty acids in foodstuffs. At present in Mongolia, there are no national policies on controlling and regulating the content of trans fatty acids in food. Our country imports 20 - 25 tones of animals and vegetable oils, annually. Since we have not had regulations for trans fatty acids content in the foodstuffs, their content is regulated or limited by the legislation of the importing countries.In the present study, we have determined the amount of the trans fatty acids contained in different food, by using a rapid FTIR spectroscopy method with attenuated total reflection (ATR-FTIR), of the absorption band at 966-968 cm-1 frequency range. Have analyzed and evaluated more than 30 samples and the content of trans fatty acids in the margarines were higher and varies between 2.3% to 10.9%. Хүнсний бүтээгдэхүүнд түргэвчилсэн аргаар тосны хүчлийн транс-изомер тодорхойлох боломж Хураангуй: Ургамлын тосыг гидрогенжүүлэх явцад үүсдэг ханаагүй тосны хүчлийн транс-изомер нь хүний эрүүл мэндэд сөрөг нөлөө үзүүлдэг нь тогтоогдсон тул хүнсний бүтээгдэхүүнд агуулагдаж байгаа тосны хүчлийн транс-изомерийн агууламжид хяналт тавих шаардлага зүй ёсоор гарч байгаа юм. Одоогийн байдлаар манай улсад хүнсний бүтээгдэхүүн дэх транс-изомерийн агууламжийг хянах хууль эрх зүйн орчин хараахан бүрэлдээгүй байна. Манай улс жил бүр ойролцоогоор 20-25 тн амьтан, ургамлын гаралтай тос импортоор оруулж ирдэг бөгөөд эдгээрт агуулагдах тосны хүчлийн транс-изомерийн агууламж нь экспортлогч улс орнуудад баримталж буй бодлогын хүрээнд хязгаарлагдаж байна.Бид энэхүү судалгаагаар хүнсний бүтээгдэхүүнд агуулагдах тосны хүчлийн транс-изомерийн агууламжийг гэрлийн бүрэн ойлтын нэмэлт төхөөрөмж бүхий нил улаан туяаны спектрийн (ATR-FTIR) түргэвчилсэн аргаар 966-968 см-1 давтамжийн мужид илрэх шингээлтийн зурвасын эрчмээр тодорхойлох боломжтой болохыг тогтоов. Судалгааны хүрээнд гуч гаруй хүнсний бүтээгдэхүүний дээжинд тосны хүчлийн транс-изомерийн агууламжийг тодорхойлсноос маргаринд агуулагдах тосны хүчлийн транс-изомерийн агууламж хамгийн өндөр буюу 2.3 % -10.9 % байв. Түлхүүр үг: гидрогенжүүлсэн тос, хүнсний бүтээгдэхүүн, тосны хүчлийн транс-изомер, ATR-FTIR.

Advances in the Chromatographic Separation and Determination of Bioactive Compounds for Assessing the Nutrient Profile of Nuts

2020 ◽  
Vol 16 ◽  
Author(s):  
Natasa P. Kalogiouri ◽  
Natalia Manousi ◽  
Erwin Rosenberg ◽  
George A. Zachariadis ◽  
Victoria F. Samanidou
Keyword(s):  
Fatty Acids ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
High Performance ◽  
Unsaturated Fatty Acids ◽  
Nutritional Deficiencies ◽  
Analytical Technique ◽  
High Volatility ◽  
Analytical Approaches

Background:: Nuts have been incorporated into guidelines for healthy eating since they contain considerable amounts of antioxidants and their effects are related to health benefits since they contribute to the prevention of nutritional deficiencies. The micronutrient characterization is based mainly on the determination of phenolics which is the most abundant class of bioactive compounds in nuts. Terpenes constitute another class of bioactive compounds that are present in nuts and show high volatility. The analysis of phenolic compounds and terpenes are very demanding tasks that require optimization of the chromatographic conditions to improve the separation of the components. Moreover, nuts are rich in unsaturated fatty acids and they are therefore considered as cardioprotective. Gas chromatography is the predominant instrumental analytical technique for the determination of derivatized fatty acids and terpenes in food matrices, while high performance liquid chromatography is currently the most popular technique for the determination of phenolic compounds Objective:: This review summarizes all the recent advances in the optimization of the chromatographic conditions for the determination of phenolic compounds, fatty acids and terpenes in nuts Conclusion:: The state-of-the art in the technology available is critically discussed, exploring new analytical approaches to reduce the time of analysis and improve the performance of the chromatographic systems in terms of precision, reproducibility, limits of detection and quantification and overall quality of the results

scholarly journals Exploitation of HPLC Analytical Method for Simultaneous Determination of Six Principal Unsaturated Fatty Acids in Oviductus Ranae Based on Quantitative Analysis of Multi-Components by Single-Marker (QAMS)

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 479
Author(s):  
Shihan Wang ◽  
Yuanshuai Gan ◽  
Hong Kan ◽  
Xinxin Mao ◽  
Yongsheng Wang
Keyword(s):  
Fatty Acids ◽  
Quantitative Analysis ◽  
Unsaturated Fatty Acids ◽  
Internal Standard ◽  
Active Components ◽  
Reliable Determination ◽  
External Standard Method ◽  
Single Marker ◽  
Oviductus Ranae

As one of the featured products in northeast China, Oviductus Ranae has been widely used as a nutritious food, which contains a variety of bioactive unsaturated fatty acids (UFAs). It is necessary to establish a scientific and reliable determination method of UFA contents in Oviductus Ranae. In this work, six principal UFAs in Oviductus Ranae, namely eicosapentaenoic acid (EPA), linolenic acid (ALA), docosahexaenoic acid (DHA), arachidonic acid (ARA), linoleic acid (LA) and oleic acid (OA), were identified using UPLC-MS/MS. The UFAs identified in Oviductus Ranae were further separated based on the optimized RP-HPLC conditions. Quantitative analysis of multi-components by single-marker (QAMS) method was implemented in content determination of EPA, ALA, DHA, ARA and OA, where LA was used as the internal standard. The experiments based on Taguchi design verified the robustness of the QAMS method on different HPLC instruments and chromatographic columns. The QAMS and external standard method (ESM) were used to calculate the UFA content of 15 batches of Oviductus Ranae samples from different regions. The relative error (r < 0.73%) and cosine coefficient showed that the two methods obtained similar contents, and the method validations met the requirements. The results showed that QAMS can comprehensively and effectively control the quality of UFAs in Oviductus Ranae which provides new ideas and solutions for studying the active components in Oviductus Ranae.

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