Identification of inflammatory markers in chronic rhinosinusitis using Raman spectroscopy (Conference Presentation)

2019 ◽  
Author(s):  
Surya P. Singh ◽  
David Zarabanda ◽  
Andrey V. Malkovskiy ◽  
Jayakar V. Nayak ◽  
Tulio A. Valdez
Keyword(s):  
Raman Spectroscopy ◽  
Chronic Rhinosinusitis ◽  
Inflammatory Markers

scholarly journals Comparison of Subtyping Approaches and the Underlying Drivers of Microbial Signatures for Chronic Rhinosinusitis

mSphere ◽  
2019 ◽  
Vol 4 (1) ◽  
Author(s):  
Kristi Biswas ◽  
Raewyn Cavubati ◽  
Shan Gunaratna ◽  
Michael Hoggard ◽  
Sharon Waldvogel-Thurlow ◽  
...  
Keyword(s):  
Tight Junction ◽  
Chronic Rhinosinusitis ◽  
Inflammatory Markers ◽  
Nasal Polyps ◽  
Epithelial Barrier ◽  
Clear Understanding ◽  
Microbial Composition ◽  
Data Set ◽  
Heterogeneous Condition ◽  
Gene And Protein Expression

ABSTRACT Chronic rhinosinusitis (CRS) is a heterogeneous condition characterized by persistent sinus inflammation and microbial dysbiosis. This study aimed to identify clinically relevant subgroups of CRS patients based on distinct microbial signatures, with a comparison to the commonly used phenotypic subgrouping approach. The underlying drivers of these distinct microbial clusters were also investigated, together with associations with epithelial barrier integrity. Sinus biopsy specimens were collected from CRS patients (n = 23) and disease controls (n = 8). The expression of 42 tight junction genes was evaluated using quantitative PCR together with microbiota analysis and immunohistochemistry for measuring mucosal integrity and inflammation. CRS patients clustered into two distinct microbial subgroups using probabilistic modelling Dirichlet (DC) multinomial mixtures. DC1 exhibited significantly reduced bacterial diversity and increased dispersion and was dominated by Pseudomonas, Haemophilus, and Achromobacter. DC2 had significantly elevated B cells and incidences of nasal polyps and higher numbers of Anaerococcus, Megasphaera, Prevotella, Atopobium, and Propionibacterium. In addition, each DC exhibited distinct tight junction gene and protein expression profiles compared with those of controls. Stratifying CRS patients based on clinical phenotypic subtypes (absence or presence of nasal polyps [CRSsNP or CRSwNP, respectively] or with cystic fibrosis [CRSwCF]) accounted for a larger proportion of the variation in the microbial data set than with DC groupings. However, no significant differences between CRSsNP and CRSwNP cohorts were observed for inflammatory markers, beta-dispersion, and alpha-diversity measures. In conclusion, both approaches used for stratifying CRS patients had benefits and pitfalls, but DC clustering provided greater resolution when studying tight junction impairment. Future studies in CRS should give careful consideration to the patient subtyping approach used. IMPORTANCE Chronic rhinosinusitis (CRS) is a major human health problem that significantly reduces quality of life. While various microbes have been implicated, there is no clear understanding of the role they play in CRS pathogenesis. Another equally important observation made for CRS patients is that the epithelial barrier in the sinonasal cavity is defective. Finding a robust approach to subtype CRS patients would be the first step toward unravelling the pathogenesis of this heterogeneous condition. Previous work has explored stratification based on the clinical presentation of the disease (with or without polyps), inflammatory markers, pathology, or microbial composition. Comparisons between the different stratification approaches used in these studies have not been possible due to the different cohorts, analytical methods, or sample sites used. In this study, two approaches for subtyping CRS patients were compared, and the underlying drivers of the heterogeneity in CRS were also explored.

Raman Spectroscopy for Inverted Papilloma: A Proof-of-Concept Study

2018 ◽  
Vol 159 (3) ◽  
pp. 587-589 ◽  
Author(s):  
Marco A. Mascarella ◽  
Abdulaziz Alrasheed ◽  
Naif Fnais ◽  
Ophelie Gourgas ◽  
Ghulam Jalani ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Raman Spectroscopy ◽  
Chronic Rhinosinusitis ◽  
Principal Component ◽  
Inverted Papilloma ◽  
Proof Of Concept ◽  
Linear Discriminant ◽  
Acceptable Accuracy ◽  
Sinonasal Mucosa ◽  
Inverted Papillomas

Inverted papillomas are tumors of the sinonasal tract with a propensity to recur. Raman spectroscopy can potentially identify inverted papillomas from other tissue based on biochemical signatures. A pilot study comparing Raman spectroscopy to histopathology for 3 types of sinonasal tissue was performed. Spectral data of biopsies from patients with normal sinonasal mucosa, chronic rhinosinusitis, and inverted papillomas are compared to histopathology using principal component analysis and linear discriminant analysis after data preprocessing. A total of 18 normal, 15 chronic rhinosinusitis, and 18 inverted papilloma specimens were evaluated. The model distinguished normal sinonasal mucosa, chronic rhinosinusitis, and inverted papilloma tissue with an overall accuracy of 90.2% (95% confidence interval, 0.86-0.94). In conclusion, Raman spectroscopy can distinguish inverted papilloma, normal sinonasal mucosa, and chronically rhinosinusitis tissue with acceptable accuracy.

scholarly journals Inflammatory markers in the nasal mucosa and polyp-modified tissues in chronic rhinosinusitis with nasal polyps

2021 ◽  
Vol 10 (3) ◽  
pp. 29-38
Author(s):  
D.G. Pavlush ◽  
◽  
I.V. Dyuizen ◽  
Keyword(s):  
Chronic Rhinosinusitis ◽  
Nasal Mucosa ◽  
Inflammatory Markers ◽  
Nasal Polyps ◽  
Chemical Structure ◽  
Comparison Group ◽  
Pathological Process ◽  
Molecular Rearrangement ◽  
Clinical Prognosis ◽  
Neurokinin Receptors

Introduction. To date, chronic rhinosinusitis with nasal polyps (CRSwNP) has not yet been extensively studied: the molecular factors and mechanisms involved in the initiation of polypous transformations in nasal mucosa (NM) and sustaining their recurrence probability are still to be determined. Simultaneously, it is necessary to understand the molecular rearrangement in NM tissues to make clinical prognosis and choose an adequate therapeutic or surgical strategy for CRSwNP treatment. The aim of the study was to identify the features of how inflammatory markers localize and are distributed in the NM and polyps in various morphological CRSwNP types. Materials and methods. We studied morphological and chemical structure of nasal polyps and mucosa of the inferior turbinates. The material was obtained during surgical management of patients diagnosed with CRSwNP. The comparison group involved the patients with a deviated septum who underwent septorhi-noplasty and had neither polyposis nor concomitant inflammatory/allergic pathology. The NM removed in surgeries was used to compare morphological and chemical changes. Immunohistochemistry was applied to determine the localization and distribution of SP, NK1, nNOS, iNOS, and IL1b in the tissues. Results. The formation of nasal polyps was found to be accompanied by morphological and chemical altera-tions in the mucous membrane of the inferior turbinates. In polyps of different morphological types, the changes in the activity of inflammatory markers were specific. Conclusion. The data obtained indicate that changes in the NM of the inferior turbinates, which accompany polyposis development, give certain pathological causes that induce and maintain the pathological process. We have revealed the features of the specific signaling microenvironment in the nasal cavity, which provide special conditions for the formation of polyps of various types. The specificity of the activity and distribu-tion of inflammatory markers in the polyps of different morphological types may serve as a prerequisite for the development of personalized therapy for the disease. Keywords: chronic rhinosinusitis with nasal polyps, inflammation, neurokinin receptors, substance P, nitric oxide

The effect of topical amphotericin B on inflammatory markers in patients with chronic rhinosinusitis: A multicenter randomized controlled study

2009 ◽  
Vol 119 (2) ◽  
pp. 401-408 ◽  
Author(s):  
Fenna A. Ebbens ◽  
Christos Georgalas ◽  
Silvia Luiten ◽  
Cornelis M. van Drunen ◽  
Lydia Badia ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Chronic Rhinosinusitis ◽  
Inflammatory Markers ◽  
Randomized Controlled Study ◽  
Controlled Study ◽  
Randomized Controlled

scholarly journals Chronic Rhinosinusitis with Nasal Polyps: Age and Disease Severity Differences in the Levels of Inflammatory Markers

Medicina ◽  
2021 ◽  
Vol 57 (3) ◽  
pp. 282
Author(s):  
Justinas Vaitkus ◽  
Astra Vitkauskienė ◽  
Regimantas Simuntis ◽  
Žygimantas Vaitkus ◽  
Nora Šiupšinskienė ◽  
...  
Keyword(s):  
Chronic Rhinosinusitis ◽  
Inflammatory Markers ◽  
Nasal Polyps ◽  
Clinical Symptoms ◽  
Age Groups ◽  
Magnetic Bead ◽  
Control Group ◽  
Control Subjects ◽  
Nasal Tissue ◽  
And Control

Background and objectives: The aim of our study was to analyze the concentrations of inflammatory markers in the nasal tissue of patients with chronic rhinosinusitis with nasal polyps (CRSwNPs) and controls of different age groups, as well as to find associations between age, inflammation development, and NPs. Materials and methods: Patients were divided into two groups—patients with CRSwNPs and control subjects who had nasal surgery for another reason beside CRS. Our analysis was performed across three different age groups (18–30 years, 31–50 years, and 51 years and more). Tissue biopsies from the sinus cavity for all study participants were taken and frozen at −80 °C, until use. The concentrations of IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-13, IL-21, and IL-22, were quantified using a magnetic bead-based multiplex assay. Results: In the group aged 18–30 years, the levels of inflammatory markers IL-1, IL-2, IL-5, and IL-22 were significantly higher in patients with CRSwNPs than the control subjects. Among patients aged 31–50 years, significantly higher concentrations of IL-2, IL-4, IL-5, and IL-22 were recorded in patients with CRSwNPs, as compared to the control subjects. In the oldest group (aged 51 years and more), patients with CRSwNPs had significantly higher concentrations of IL-2, IL-4, and IL-22, as compared to the control group. In the CRSwNP group, only the concentration of IL-21 was significantly higher among patients aged 31–50 years, as compared with those aged 51 years and older (p = 0.013). Conclusions: IL-2 and IL-22 levels were significantly higher in patients with CRSwNP than the control, across all age groups. Only the concentration of IL-21 was higher among patients with CRSwNP in the middle age group, as compared to the oldest group. IL-2, IL-4, and IL-22 levels correlated with the severity of CRSwNPs. Elevated concentrations of IL-2, IL-4, and IL-22 were determined in patients’ groups with higher sinonasal outcome test (SNOT-22) scores, pointing to more severe clinical symptoms.

γδT cells contribute to type 2 inflammatory profiles in eosinophilic chronic rhinosinusitis with nasal polyps

2019 ◽  
Vol 133 (22) ◽  
pp. 2301-2315 ◽  
Author(s):  
Xia Li ◽  
Zhiyuan Wang ◽  
Lihong Chang ◽  
Xiaohong Chen ◽  
Luoying Yang ◽  
...  
Keyword(s):  
Chronic Rhinosinusitis ◽  
Murine Model ◽  
Inflammatory Markers ◽  
Nasal Polyps ◽  
Γδt Cells ◽  
Control Subjects ◽  
Nasal Tissue ◽  
Type 2 Cytokines ◽  
Eosinophilic Chronic Rhinosinusitis

Abstract Eosinophilic chronic rhinosinusitis with nasal polyps (ECRS) is a condition linked with type 2 inflammation, poor treatment outcomes, and high recurrence tendency. Although γδT cells have been reported to induce type 2 immune responses and eosinophilic infiltration in several diseases, their role in ECRS has not been fully explored. We aimed to evaluate the association of γδT cells with the type 2 inflammatory profiles in ECRS. Nasal tissue samples obtained from patients with chronic rhinosinusitis with nasal polyps (CRSwNP) (51 eosinophilic and 48 non-eosinophilic), 50 patients with chronic rhinosinusitis without nasal polyps (CRSsNP), and 58 control subjects were examined for γδT cells, inflammatory markers and eosinophils using HE, RT-qPCR, ELISA, immunofluorescence, and flow cytometry. In parallel, studies were also conducted in an ECRS murine model induced by anti-γδT cells neutralizing antibody administration. γδT cells expression was significantly increased in tissues from patients with ECRS compared with non-ECRS, CRSsNP and control subjects. Moreover, inflammatory markers including type 2 proinflammatory cytokines (IL-4, IL-5, IL-13), GATA3, eosinophil cationic protein (ECP), and eotaxin levels were also increased in nasal tissues of patients with ECRS, and Vγ1+ γδT cells mRNA expression was positively correlated with type 2 cytokines, GATA3, and ECP. In the ECRS murine model, anti-Vγ1+ γδT antibody treatment reduced the infiltration of eosinophils and expression of type 2 cytokines, GATA3, and ECP in nasal mucosae. In conclusion, the results of the present study suggest that γδT cells play a crucial role in the type 2 inflammatory profiles and nasal tissue eosinophilic infiltration in patients with ECRS.

scholarly journals Use of endotypes, phenotypes, and inflammatory markers to guide treatment decisions in chronic rhinosinusitis

2020 ◽  
Vol 124 (4) ◽  
pp. 318-325 ◽  
Author(s):  
Anna G. Staudacher ◽  
Anju T. Peters ◽  
Atsushi Kato ◽  
Whitney W. Stevens
Keyword(s):  
Chronic Rhinosinusitis ◽  
Inflammatory Markers ◽  
Treatment Decisions
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