In-vivo deep-brain imaging through a single fibre endoscope (Conference Presentation)

2019 ◽  
Author(s):  
Sergey Turtaev ◽  
Ivo T. Leite ◽  
Tristan Altwegg-Boussac ◽  
Janelle M. P. Pakan ◽  
Nathalie L. Rochefort ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Single Fibre ◽  
Deep Brain

scholarly journals Adaptive optics two-photon endomicroscopy enables deep-brain imaging at synaptic resolution over large volumes

2020 ◽  
Vol 6 (40) ◽  
pp. eabc6521 ◽  
Author(s):  
Zhongya Qin ◽  
Congping Chen ◽  
Sicong He ◽  
Ye Wang ◽  
Kam Fai Tam ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Adaptive Optics ◽  
Critical Role ◽  
Random Access ◽  
Brain Structures ◽  
Invasive Approach ◽  
Two Photon ◽  
Grin Lens ◽  
Deep Brain

Optical deep-brain imaging in vivo at high resolution has remained a great challenge over the decades. Two-photon endomicroscopy provides a minimally invasive approach to image buried brain structures, once it is integrated with a gradient refractive index (GRIN) lens embedded in the brain. However, its imaging resolution and field of view are compromised by the intrinsic aberrations of the GRIN lens. Here, we develop a two-photon endomicroscopy by adding adaptive optics based on direct wavefront sensing, which enables recovery of diffraction-limited resolution in deep-brain imaging. A new precompensation strategy plays a critical role to correct aberrations over large volumes and achieve rapid random-access multiplane imaging. We investigate the neuronal plasticity in the hippocampus, a critical deep brain structure, and reveal the relationship between the somatic and dendritic activity of pyramidal neurons.

scholarly journals In vivo deep brain imaging of rats using oral-cavity illuminated photoacoustic computed tomography

2015 ◽  
Author(s):  
Li Lin ◽  
Jun Xia ◽  
Terence T. W. Wong ◽  
Ruiying Zhang ◽  
Lihong V. Wang
Keyword(s):  
Computed Tomography ◽  
Oral Cavity ◽  
Brain Imaging ◽  
Deep Brain

scholarly journals Reconnectable fiberscopes for chronic in vivo deep-brain imaging

2018 ◽  
Vol 11 (4) ◽  
pp. e201700106 ◽  
Author(s):  
M. S. Pochechuev ◽  
I. V. Fedotov ◽  
O. I. Ivashkina ◽  
M. A. Roshchina ◽  
D.V. Meshchankin ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Deep Brain

scholarly journals Theranostic OCT microneedle for fast ultrahigh-resolution deep-brain imaging and efficient laser ablation in vivo

2020 ◽  
Vol 6 (15) ◽  
pp. eaaz9664 ◽  
Author(s):  
Wu Yuan ◽  
Defu Chen ◽  
Rachel Sarabia-Estrada ◽  
Hugo Guerrero-Cázares ◽  
Dawei Li ◽  
...  
Keyword(s):  
Laser Ablation ◽  
Brain Imaging ◽  
Mouse Brain ◽  
High Speed ◽  
Continuous Wave ◽  
Brain Diseases ◽  
Outer Diameter ◽  
Ultrahigh Resolution ◽  
Deep Brain

Current minimally invasive optical techniques for in vivo deep-brain imaging provide a limited resolution, field of view, and speed. These limitations prohibit direct assessment of detailed histomorphology of various deep-seated brain diseases at their native state and therefore hinder the potential clinical utilities of those techniques. Here, we report an ultracompact (580 μm in outer diameter) theranostic deep-brain microneedle combining 800-nm optical coherence tomography imaging with laser ablation. Its performance was demonstrated by in vivo ultrahigh-resolution (1.7 μm axial and 5.7 μm transverse), high-speed (20 frames per second) volumetric imaging of mouse brain microstructures and optical attenuation coefficients. Its translational potential was further demonstrated by in vivo cancer visualization (with an imaging depth of 1.23 mm) and efficient tissue ablation (with a 1448-nm continuous-wave laser at a 350-mW power) in a deep mouse brain (with an ablation depth of about 600 μm).

scholarly journals Minimally invasive deep-brain imaging through a 50 μm-core multimode fibre

2018 ◽  
Author(s):  
Sebastian A. Vasquez-Lopez ◽  
Vadim Koren ◽  
Martin Plöschner ◽  
Zahid Padamsey ◽  
Tomáš Čižmár ◽  
...  
Keyword(s):  
High Resolution ◽  
Brain Imaging ◽  
Light Propagation ◽  
Neural Circuit ◽  
Complex Refractive Index ◽  
Brain Structures ◽  
Brain Regions ◽  
Long Distance ◽  
Deep Brain

AbstractAchieving optical access to deep-brain structures represents an important step towards the goal of understanding the mammalian central nervous system. The complex refractive index distribution within brain tissue introduces severe aberrations to long-distance light propagation thereby prohibiting image reconstruction using currently available non-invasive techniques. In an attempt to overcome this challenge endoscopic approaches have been adopted, principally in the form of fibre bundles or GRIN-lens based endoscopes. Unfortunately, these approaches create substantial mechanical lesions of the tissue precipitating neuropathological responses that include inflammation and gliosis. Together, lesions and the associated neuropathology may compromise neural circuit performance. By replacing Fourier-based image relay with a holographic approach, we have been able to reduce the volume of tissue lesion by more than 100-fold, while preserving diffraction-limited imaging performance. Here we demonstrate high-resolution fluorescence imaging of neuronal structures, dendrites and synaptic specialisations, in deep-brain regions of living mice. These results represent a major breakthrough in the compromise between high-resolution imaging and tissue damage, heralding new possibilities for deep-brain imaging in vivo.

scholarly journals Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber

2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Sebastian A. Vasquez-Lopez ◽  
Raphaël Turcotte ◽  
Vadim Koren ◽  
Martin Plöschner ◽  
Zahid Padamsey ◽  
...  
Keyword(s):  
Minimally Invasive ◽  
Brain Imaging ◽  
Spatial Resolution ◽  
Multimode Fiber ◽  
Deep Brain

In vivo deep-brain imaging of microglia enabled by three-photon fluorescence microscopy

2020 ◽  
Vol 45 (18) ◽  
pp. 5271
Author(s):  
Hui Cheng ◽  
Shen Tong ◽  
Xiangquan Deng ◽  
Jia Li ◽  
Ping Qiu ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Brain Imaging ◽  
Photon Fluorescence ◽  
Deep Brain

scholarly journals Fluorescence microendoscopy for in vivo deep-brain imaging of neuronal circuits

2021 ◽  
Vol 348 ◽  
pp. 109015
Author(s):  
Brenton T. Laing ◽  
Justin N. Siemian ◽  
Sarah Sarsfield ◽  
Yeka Aponte
Keyword(s):  
Brain Imaging ◽  
Neuronal Circuits ◽  
Deep Brain
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