Characterization of oviduct ciliary beat frequency using spectrally encoded interferometric microscopy (Conference Presentation)

2019 ◽  
Author(s):  
Yueqiao Qu ◽  
Zhongping Chen ◽  
Youmin He ◽  
Joseph Jing
Keyword(s):  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Spectrally Encoded ◽  
Ciliary Beat

scholarly journals Characterization of an A-Kinase Anchoring Protein in Human Ciliary Axonemes

2002 ◽  
Vol 13 (12) ◽  
pp. 4156-4166 ◽  
Author(s):  
Patricia L. Kultgen ◽  
Sherell K. Byrd ◽  
Lawrence E. Ostrowski ◽  
Sharon L. Milgram
Keyword(s):  
Protein Kinase ◽  
Molecular Mechanisms ◽  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Anchoring Proteins ◽  
Anchoring Protein ◽  
Airway Cilia ◽  
Kinase A ◽  
Ciliary Beat

Although protein kinase A (PKA) activation is known to increase ciliary beat frequency in humans the molecular mechanisms involved are unknown. We demonstrate that PKA is associated with ciliary axonemes where it specifically phosphorylates a 23-kDa protein. Because PKA is often localized to subcellular compartments in proximity to its substrate(s) via interactions with A-kinase–anchoring proteins (AKAPs), we investigated whether an AKAP was also associated with ciliary axonemes. This study has identified a novel 28 kDa AKAP (AKAP28)that is highly enriched in airway axonemes. The mRNA for AKAP28 is up-regulated as primary airway cells differentiate and is specifically expressed in tissues containing cilia and/or flagella. Additionally, both Western blot and immunostaining data show that AKAP28 is enriched in airway cilia. These data demonstrate that we have identified the first human axonemal AKAP, a protein that likely plays a role in the signaling necessary for efficient modulation of ciliary beat frequency.

scholarly journals Spatial Mapping of Tracheal Ciliary Beat Frequency Using Real Time Phase-Resolved Doppler Spectrally Encoded Interferometric Microscopy

ACS Photonics ◽  
2019 ◽  
Vol 7 (1) ◽  
pp. 128-134
Author(s):  
Youmin He ◽  
Joseph C. Jing ◽  
Yueqiao Qu ◽  
Brian J. Wong ◽  
Zhongping Chen
Keyword(s):  
Real Time ◽  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Spatial Mapping ◽  
Spectrally Encoded ◽  
Ciliary Beat

Effects of Prostaglandins D, and E2on Ciliary Beat Frequency of Human Upper Respiratory Ciliain Vitro

1995 ◽  
Vol 115 (3) ◽  
pp. 438-442 ◽  
Author(s):  
P. J. Schuil ◽  
M. Ten Berge ◽  
J. M. E. Van Gelder ◽  
K. Graamans ◽  
E. H. Huizing
Keyword(s):  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Upper Respiratory ◽  
Ciliary Beat

Apparatus for endoscopical, laser-based determination of ciliary beat frequency

2005 ◽  
Author(s):  
N. Bogdanovic ◽  
B. Krattiger ◽  
J. Ricka ◽  
M. Frenz
Keyword(s):  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Ciliary Beat

Betadine has a ciliotoxic effect on ciliated human respiratory cells

2014 ◽  
Vol 129 (S1) ◽  
pp. S45-S50 ◽  
Author(s):  
J H Kim ◽  
J Rimmer ◽  
N Mrad ◽  
S Ahmadzada ◽  
R J Harvey
Keyword(s):  
Epithelial Cells ◽  
High Speed ◽  
Imaging System ◽  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Positive Control ◽  
Clinical Dose ◽  
Significant Difference ◽  
Sinonasal Mucosa ◽  
Ciliary Beat

AbstractObjective:This study investigated the effect of Betadine on ciliated human respiratory epithelial cells.Methods:Epithelial cells from human sinonasal mucosa were cultured at the air–liquid interface. The cultures were tested with Hanks' balanced salt solution containing 10 mM HEPES (control), 100 µM ATP (positive control), 5 per cent Betadine or 10 per cent Betadine (clinical dose). Ciliary beat frequency was analysed using a high-speed camera on a computer imaging system.Results:Undiluted 10 per cent Betadine (n = 6) decreased the proportion of actively beating cilia over 1 minute (p < 0.01). Ciliary beat frequency decreased from 11.15 ± 4.64 Hz to no detectable activity. The result was similar with 5 per cent Betadine (n = 7), with no significant difference compared with the 10 per cent solution findings.Conclusion:Betadine, at either 5 and 10 per cent, was ciliotoxic. Caution should be applied to the use of topical Betadine solution on the respiratory mucosal surface.

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