Measuring cytochrome c redox state using resonance Raman spectroscopy to determine metabolic rates in electron transport chain when exposed to light

2019 ◽  
Author(s):  
Joshua W. Lalonde ◽  
Sean P. O'Connor ◽  
Gary D. Noojin ◽  
Cherry C. Gonzales ◽  
Vladislav V. Yakovlev ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Electron Transport ◽  
Cytochrome C ◽  
Electron Transport Chain ◽  
Redox State ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman ◽  
Metabolic Rates ◽  
Transport Chain

Resonance Raman spectroscopy of the cytochrome c oxidase from Paracoccus denitrificans

Biochemistry ◽  
1993 ◽  
Vol 32 (27) ◽  
pp. 6923-6927 ◽  
Author(s):  
Stephen R. Lynch ◽  
Richard H. Carter ◽  
Robert A. Copeland
Keyword(s):  
Raman Spectroscopy ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Paracoccus Denitrificans ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman

scholarly journals Effects of L-Malate on Mitochondrial Oxidoreductases in Liver of Aged Rats

2011 ◽  
pp. 329-336 ◽  
Author(s):  
J.-L. WU ◽  
Q.-P. WU ◽  
Y.-P. PENG ◽  
J.-M. ZHANG
Keyword(s):  
Electron Transport ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Electron Transport Chain ◽  
Nadh Dehydrogenase ◽  
Tricarboxylic Acid Cycle ◽  
Radical Scavenger ◽  
Aged Rats ◽  
Transport Chain ◽  
Nadh Cytochrome

Accumulation of oxidative damage has been implicated to be a major causative factor in the decline in physiological functions that occur during the aging process. The mitochondrial respiratory chain is a powerful source of reactive oxygen species (ROS), considered as the pathogenic agent of many diseases and aging. L-malate, a tricarboxylic acid cycle intermediate, plays an important role in transporting NADH from cytosol to mitochondria for energy production. Previous studies in our laboratory reported L-malate as a free radical scavenger in aged rats. In the present study we focused on the effect of L-malate on the activities of electron transport chain in young and aged rats. We found that mitochondrial membrane potential (MMP) and the activities of succinate dehydrogenase, NADH-cytochrome c oxidoreductase and cytochrome c oxidase in liver of aged rats were significantly decreased when compared to young control rats. Supplementation of L-malate to aged rats for 30 days slightly increased MMP and improved the activities of NADH-dehydrogenase, NADH-cytochrome c oxidoreductase and cytochrome c oxidase in liver of aged rats when compared with aged control rats. In young rats, L-malate administration increased only the activity of NADH-dehydrogenase. Our result suggested that L-malate could improve the activities of electron transport chain enzymes in aged rats

Electron Transfer Kinetics of Cytochrome c Probed by Time-Resolved Surface-Enhanced Resonance Raman Spectroscopy

2009 ◽  
Vol 113 (8) ◽  
pp. 2492-2497 ◽  
Author(s):  
Marc Grosserueschkamp ◽  
Marcel G. Friedrich ◽  
Markus Plum ◽  
Wolfgang Knoll ◽  
Renate L. C. Naumann
Keyword(s):  
Raman Spectroscopy ◽  
Electron Transfer ◽  
Cytochrome C ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman ◽  
Electron Transfer Kinetics ◽  
Time Resolved ◽  
Surface Enhanced ◽  
Kinetics Of

Resonance Raman spectroscopy of cytochrome c peroxidase variants that mimic manganese peroxidase

2003 ◽  
Vol 8 (7) ◽  
pp. 699-706 ◽  
Author(s):  
Manliang Feng ◽  
Hiroyasu Tachikawa ◽  
Xiaotang Wang ◽  
Thomas D. Pfister ◽  
Alan J. Gengenbach ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Cytochrome C ◽  
Manganese Peroxidase ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman ◽  
Cytochrome C Peroxidase
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