Visualizing and enhancing axial resolution in nonconfocal adaptive optics ophthalmoscopy

2019 ◽  
Author(s):  
Pedro . Mecê ◽  
Elena Gofas-Salas ◽  
Kate Grieve ◽  
Cyril Petit ◽  
Frédéric Cassaing ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Axial Resolution

scholarly journals Full three-dimensional imaging deep through multicellular thick samples with subcellular resolution by structured illumination microscopy and adaptive optics

2020 ◽  
Author(s):  
Ruizhe Lin ◽  
Edward T. Kipreos ◽  
Jie Zhu ◽  
Chang Hyun Khang ◽  
Peter Kner
Keyword(s):  
Adaptive Optics ◽  
Three Dimensional ◽  
Structured Illumination ◽  
Axial Resolution ◽  
Structured Illumination Microscopy ◽  
Optical Aberrations ◽  
C Elegans ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Subcellular Resolution

AbstractStructured Illumination Microscopy enables live imaging with resolutions of ~120 nm. Unfortunately, optical aberrations can lead to loss of resolution and artifacts in Structured Illumination Microscopy rendering the technique unusable in samples thicker than a single cell. Here we report on the combination of Adaptive Optics and Structured Illumination Microscopy enabling imaging with 140 nm lateral and 585 nm axial resolution in tissue culture cells, C. elegans, and rice blast fungus. We demonstrate that AO improves resolution and reduces artifacts, making full 3D SIM possible in thicker samples.

scholarly journals Axial resolution improvement of two-photon microscopy by multi-frame reconstruction and adaptive optics

2020 ◽  
Vol 11 (11) ◽  
pp. 6634
Author(s):  
Shiwei Ye ◽  
Yixuan Yin ◽  
Jing Yao ◽  
Jun Nie ◽  
Yuchen Song ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Axial Resolution ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Resolution Improvement

scholarly journals Higher adaptive optics loop rate enhances axial resolution in nonconfocal ophthalmoscopes

2019 ◽  
Vol 44 (9) ◽  
pp. 2208 ◽  
Author(s):  
Pedro Mecê ◽  
Elena Gofas-Salas ◽  
Cyril Petit ◽  
Frédéric Cassaing ◽  
José Sahel ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Axial Resolution

scholarly journals Subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Ruizhe Lin ◽  
Edward T. Kipreos ◽  
Jie Zhu ◽  
Chang Hyun Khang ◽  
Peter Kner
Keyword(s):  
Caenorhabditis Elegans ◽  
Single Cell ◽  
Adaptive Optics ◽  
Biological Samples ◽  
Three Dimensional ◽  
Axial Direction ◽  
Structured Illumination ◽  
Axial Resolution ◽  
Structured Illumination Microscopy ◽  
Optical Aberrations

AbstractStructured Illumination Microscopy enables live imaging with sub-diffraction resolution. Unfortunately, optical aberrations can lead to loss of resolution and artifacts in Structured Illumination Microscopy rendering the technique unusable in samples thicker than a single cell. Here we report on the combination of Adaptive Optics and Structured Illumination Microscopy enabling imaging with 150 nm lateral and 570 nm axial resolution at a depth of 80 µm through Caenorhabditis elegans. We demonstrate that Adaptive Optics improves the three-dimensional resolution, especially along the axial direction, and reduces artifacts, successfully realizing 3D-Structured Illumination Microscopy in a variety of biological samples.

PERFORMANCE AND AXIAL RESOLUTION OF THE ADAPTIVE OPTICS SCANNING LASER OPHTHALMOSCOPE.

2002 ◽  
Vol 79 (Supplement) ◽  
pp. 200
Author(s):  
Austin Roorda ◽  
Krishnakumar Venkateswaran ◽  
Fernando Romero-Borja ◽  
Thomas Hebert
Keyword(s):  
Adaptive Optics ◽  
Scanning Laser Ophthalmoscope ◽  
Scanning Laser ◽  
Axial Resolution

Accounting for mirror dynamics in optimal Adaptive Optics control

2009 ◽  
Author(s):  
Carlos Correia ◽  
Henri-Francois Raynaud ◽  
Caroline Kulcsar ◽  
Jean-Marc Conan
Keyword(s):  
Adaptive Optics
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