Pre-resonance stimulated Raman scattering spectroscopy and imaging of membrane potential using near-infrared rhodopsins

2019 ◽  
Author(s):  
Hyeon Jeong Lee ◽  
Ji-Xin Cheng ◽  
Kai-Chih Huang ◽  
Gaoxiang Mei ◽  
Natalia Mamaeva ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Raman Scattering ◽  
Near Infrared ◽  
Stimulated Raman Scattering ◽  
Raman Scattering Spectroscopy ◽  
Stimulated Raman

Advancing Stimulated Raman Scattering spectroscopy using Squeezed Light

2021 ◽  
Author(s):  
Rayssa B. de Andrade ◽  
Kirstine Berg-Sorensen ◽  
Tobias Gehring ◽  
Ulrik L. Andersen
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Raman Scattering Spectroscopy ◽  
Squeezed Light ◽  
Stimulated Raman

scholarly journals Broadband stimulated Raman scattering spectroscopy by a photonic time stretcher

2016 ◽  
Vol 24 (19) ◽  
pp. 21264 ◽  
Author(s):  
Francesco Saltarelli ◽  
Vikas Kumar ◽  
Daniele Viola ◽  
Francesco Crisafi ◽  
Fabrizio Preda ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Raman Scattering Spectroscopy ◽  
Stimulated Raman

scholarly journals Mapping Solvation Heterogeneity in Live Cells by Hyperspectral Stimulated Raman Scattering Microscopy

2020 ◽  
Author(s):  
Xiaoqi Lang ◽  
Kevin Welsher
Keyword(s):  
Raman Scattering ◽  
Near Infrared ◽  
Stimulated Raman Scattering ◽  
Live Cells ◽  
Spatiotemporal Resolution ◽  
Environmental Variance ◽  
Dynamic Matrix ◽  
Coherent Raman ◽  
Living Organisms ◽  
Stimulated Raman

<p>Water provides a dynamic matrix in which all biochemical processes occur in living organisms. The structure and dynamics of intracellular water constitute the cornerstone for understanding all aspects of cellular function. Fundamentally, direct visualization of subcellular solvation heterogeneity is essential but remains challenging with commonly used NMR methods due to poor spatial resolution. To explore this question, we demonstrate a vibrational-shift imaging approach by combining the spectral-focusing hyperspectral stimulated Raman scattering (hsSRS) technique with an environmentally-sensitive nitrile probe. The sensing ability of a near-infrared nitrile-containing molecule is validated in the solution phase, microscopic droplets and cellular environments. Finally, we quantitatively measure the subcellular solvation variance between the cytoplasm (29.5%, S.E. 1.8%) and the nucleus (57.3%, S.E. 1.0%), which is in good agreement with previous studies. This work sheds light on heterogenous solvation in live systems using coherent Raman microscopy and opens up new avenues to explore environmental variance in complex systems with high spatiotemporal resolution.</p>

Mapping Solvation Heterogeneity in Live Cells by Hyperspectral Stimulated Raman Scattering Microscopy

2019 ◽  
Author(s):  
Xiaoqi Lang ◽  
Kevin Welsher
Keyword(s):  
Raman Scattering ◽  
Near Infrared ◽  
Stimulated Raman Scattering ◽  
Free Water ◽  
Live Cells ◽  
Spatiotemporal Resolution ◽  
Environmental Variance ◽  
Dynamic Matrix ◽  
Coherent Raman ◽  
Stimulated Raman

<div><div><div><p>Water provides a dynamic matrix in which all biochemical processes occur in living organisms. The structure and dynamics of intracellular water constitute the cornerstone for understanding all aspects of cellular function. Fundamentally, direct visualization of subcellular solvation heterogeneity is essential but remains challenging with commonly used NMR methods due to poor spatial resolution. To explore this question, we demonstrate a vibrational-shift imaging approach by combining the spectral-focusing hyperspectral stimulated Raman scattering (hsSRS) technique with an environmentally-sensitive nitrile probe. The sensing ability of a near-infrared nitrile-containing molecule is validated in the solution phase, microscopic droplets and cellular environments. Finally, we quantitatively measure the subcellular solvation variance in the degree of free water content between the cytoplasm (29.7%, S.E. 1.1%) and the nucleus (56.4%, S.E. 1.3%), which is in good agreement with previous studies. This work sheds light on mapping the heterogenous solvation in live systems using coherent Raman microscopy and opens up new avenues to explore environmental variance in complex systems with high spatiotemporal resolution.</p></div></div></div>

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