Multiplexed detection of CRP-SAA1 using laser direct-write-fabricated multiple flow-path lateral flow devices (Conference Presentation)

2019 ◽  
Author(s):  
Peijun He ◽  
Ioannis . Katis ◽  
Panagiotis Galanis ◽  
Jessica Teeling ◽  
Clive Holmes ◽  
...  
Keyword(s):  
Flow Path ◽  
Lateral Flow ◽  
Direct Write ◽  
Multiplexed Detection ◽  
Flow Devices ◽  
Laser Direct Write

scholarly journals Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique

Biosensors ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 97 ◽  
Author(s):  
Peijun He ◽  
Ioannis Katis ◽  
Robert Eason ◽  
Collin Sones
Keyword(s):  
Bacterial Infections ◽  
Point Of Care ◽  
Simultaneous Detection ◽  
Cross Reactivity ◽  
Lateral Flow ◽  
Direct Write ◽  
Multiplexed Detection ◽  
Amyloid A ◽  
Flow Devices ◽  
Laser Direct Write

Paper-based lateral flow devices (LFDs) are regarded as ideal low-cost diagnostic solutions for point-of-care (POC) scenarios that allow rapid detection of a single analyte within a fluidic sample, and have been in common use for a decade. In recent years, there has been an increasing need for rapid and simultaneous detection of multiple analytes present within a single sample and to facilitate this, we report here a novel solution—detection using a multi-path LFD created via the precise partitioning of the single flow-path of a standard LFD using our previously reported laser direct-write (LDW) technique. The multiple flow-paths allow the simultaneous detection of the different analytes individually within each of the parallel channels without any cross-reactivity. The appearance of coloured test lines in individual channels indicates the presence of the different analytes within a sample. We successfully present the use of a LDW-patterned multi-path LFD for multiplexed detection of a biomarker panel comprising C-reactive protein (CRP) and Serum amyloid A-1 (SAA1), used for the diagnosis of bacterial infections. Overall, we demonstrate the use of our LDW technique in the creation of a novel LFD that enables multiplexed detection of two inflammation markers within a single LFD providing a detection protocol that is comparatively more efficient than the standard sequential multiplexing procedure.

scholarly journals A rapid diagnostic test for human Visceral Leishmaniasis using novel Leishmania antigens in a Laser Direct-Write Lateral Flow Device

2019 ◽  
Vol 8 (1) ◽  
pp. 1178-1185 ◽  
Author(s):  
Maria Victoria Humbert ◽  
Lourena Emanuele Costa ◽  
Ioannis Katis ◽  
Fernanda Fonseca Ramos ◽  
Amanda Sanchéz Machado ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Lateral Flow ◽  
Direct Write ◽  
Lateral Flow Device ◽  
Human Visceral Leishmaniasis ◽  
Flow Device ◽  
Laser Direct Write

scholarly journals Improved sensitivity and limit-of-detection of lateral flow devices using spatial constrictions of the flow-path

2018 ◽  
Vol 113 ◽  
pp. 95-100 ◽  
Author(s):  
Ioannis N. Katis ◽  
Peijun J.W. He ◽  
Robert W. Eason ◽  
Collin L. Sones
Keyword(s):  
Limit Of Detection ◽  
Flow Path ◽  
Lateral Flow ◽  
Flow Devices

Redistribution of pads on a singulated IC die using laser direct-write ablation

2006 ◽  
Vol 29 (1) ◽  
pp. 184-189
Author(s):  
Chengping Zhang ◽  
R. Bartholomew ◽  
P.C. Karulkar
Keyword(s):  
Direct Write ◽  
Laser Direct Write

Picoliter-Scale Protein Microarrays by Laser Direct Write

2002 ◽  
Vol 18 (5) ◽  
pp. 1126-1129 ◽  
Author(s):  
B.R. Ringeisen ◽  
P.K. Wu ◽  
H. Kim ◽  
A. Pique ◽  
R.Y.C. Auyeung ◽  
...  
Keyword(s):  
Protein Microarrays ◽  
Direct Write ◽  
Laser Direct Write

Rapid prototyping of micropower sources by laser direct-write

2004 ◽  
Vol 79 (4-6) ◽  
pp. 783-786 ◽  
Author(s):  
A. Piqué ◽  
C.B. Arnold ◽  
H. Kim ◽  
M. Ollinger ◽  
T.E. Sutto
Keyword(s):  
Rapid Prototyping ◽  
Direct Write ◽  
Laser Direct Write

Fabrication of Hybrid Cell-Microbead Constructs Using Laser Direct-Write of Alginate Microbeads and Adherent Breast Cancer Cells

2013 ◽  
Author(s):  
Andrew D. Dias ◽  
David M. Kingsley ◽  
Douglas B. Chrisey ◽  
David T. Corr
Keyword(s):  
Drug Delivery ◽  
Mammalian Cells ◽  
Hybrid Cell ◽  
High Surface ◽  
High Surface Area ◽  
Volume Ratio ◽  
Direct Write ◽  
Cellular Interactions ◽  
Paracrine Signaling ◽  
Laser Direct Write

Microbeads are becoming popular tools in tissue engineering as 3D microstructure hydrogels. The gel nature of microbeads enables them to sequester soluble factors and mammalian cells, and their high surface area-to-volume ratio allows diffusion between the bead and the environment [1,2]. Microbeads are thus good systems for drug delivery and can serve as 3D microenvironments for cells. To fully maximize their potential as delivery systems and microenvironments, it is highly desirable to create spatially-precise hybrid cultures of microbeads and mammalian cells. Precise placement of microbeads in proximity to patterned cells will allow the study of spatial cellular interactions, paracrine signaling, and drug delivery.

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