Macrophage-mediated delivery of chemotherapeutics for photochemical internalization (Conference Presentation)

2019 ◽  
Author(s):  
Stephanie Molina ◽  
Henry Hirschberg ◽  
Steen J. Madsen
Keyword(s):  
Photochemical Internalization

scholarly journals Photocontrolled apoptosis induction using precursor miR-664a and an RNA carrier-conjugated with photosensitizer

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kazunori Watanabe ◽  
Tomoko Nawachi ◽  
Ruriko Okutani ◽  
Takashi Ohtsuki
Keyword(s):  
Cancer Therapy ◽  
Cell Types ◽  
Drug Candidate ◽  
Apoptosis Induction ◽  
Delivery Method ◽  
Apoptotic Pathway ◽  
Photochemical Internalization ◽  
Therapeutic Uses ◽  
Induction Of Apoptosis ◽  
Nucleic Acid Drug

AbstractMethods to spatially induce apoptosis are useful for cancer therapy. To control the induction of apoptosis, methods using light, such as photochemical internalization (PCI), have been developed. We hypothesized that photoinduced delivery of microRNAs (miRNAs) that regulate apoptosis could spatially induce apoptosis. In this study, we identified pre-miR-664a as a novel apoptosis-inducing miRNA via mitochondrial apoptotic pathway. Further, we demonstrated the utility of photoinduced cytosolic dispersion of RNA (PCDR), which is an intracellular RNA delivery method based on PCI. Indeed, apoptosis is spatially regulated by pre-miR-664a and PCDR. In addition, we found that apoptosis induced by pre-miR-664a delivered by PCDR was more rapid than that by lipofection. These results suggest that pre-miR-664a is a nucleic acid drug candidate for cancer therapy and PCDR and pre-miR-664a-based strategies have potential therapeutic uses for diseases affecting various cell types.

TNFα siRNA delivery by nanoparticles and photochemical internalization for psoriasis topical therapy

2021 ◽  
Author(s):  
Isabella Suzuki ◽  
Margarete Moreno de Araujo ◽  
Vanderlei Salvador Bagnato ◽  
Maria Vitoria Lopes Badra Bentley
Keyword(s):  
Topical Therapy ◽  
Sirna Delivery ◽  
Photochemical Internalization

Photochemical Internalization of Tamoxifens Transported by a “Trojan-Horse” Nanoconjugate into Breast-Cancer Cell Lines

2015 ◽  
Vol 54 (16) ◽  
pp. 4885-4889 ◽  
Author(s):  
Theodossis A. Theodossiou ◽  
A. Ricardo Gonçalves ◽  
Konstantina Yannakopoulou ◽  
Ellen Skarpen ◽  
Kristian Berg
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Trojan Horse ◽  
Breast Cancer Cell Lines ◽  
Photochemical Internalization

scholarly journals Photochemical Internalization of Etoposide Using Dendrimer Nanospheres Loaded with Etoposide and Protoporphyrin IX on a Glioblastoma Cell Line

Pharmaceutics ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1877
Author(s):  
Martin Hsiu-Chu Lin ◽  
Li-Ching Chang ◽  
Chiu-Yen Chung ◽  
Wei-Chao Huang ◽  
Ming-Hsueh Lee ◽  
...  
Keyword(s):  
Cell Line ◽  
Topoisomerase Ii ◽  
Protoporphyrin Ix ◽  
Release Mechanism ◽  
Light Illumination ◽  
Disruptive Effect ◽  
Photochemical Internalization ◽  
Polyamidoamine Dendrimers ◽  
Primary Neoplasm ◽  
Human Gbm

Glioblastoma multiforme (GBM) is the most common malignant primary neoplasm of the adult central nervous system originating from glial cells. The prognosis of those affected by GBM has remained poor despite advances in surgery, chemotherapy, and radiotherapy. Photochemical internalization (PCI) is a release mechanism of endocytosed therapeutics into the cytoplasm, which relies on the membrane disruptive effect of light-activated photosensitizers. In this study, phototherapy by PCI was performed on a human GBM cell-line using the topoisomerase II inhibitor etoposide (Etop) and the photosensitizer protoporphyrin IX (PpIX) loaded in nanospheres (Ns) made from generation-5 polyamidoamine dendrimers (PAMAM(G5)). The resultant formulation, Etop/PpIX-PAMAM(G5) Ns, measured 217.4 ± 2.9 nm in diameter and 40.5 ± 1.3 mV in charge. Confocal microscopy demonstrated PpIX fluorescence within the endo-lysosomal compartment, and an almost twofold increase in cellular uptake compared to free PpIX by flow cytometry. Phototherapy with 3 min and 5 min light illumination resulted in a greater extent of synergism than with co-administered Etop and PpIX; notably, antagonism was observed without light illumination. Mechanistically, significant increases in oxidative stress and apoptosis were observed with Etop/PpIX-PAMAM(G5) Ns upon 5 min of light illumination in comparison to treatment with either of the agents alone. In conclusion, simultaneous delivery and endo-lysosomal co-localization of Etop and PpIX by PAMAM(G5) Ns leads to a synergistic effect by phototherapy; in addition, the finding of antagonism without light illumination can be advantageous in lowering the dark toxicity and improving photo-selectivity.

Photochemical Internalization of Bleomycin is Superior to Photodynamic Therapy Due to the Therapeutic Effect in the Tumor Periphery

2009 ◽  
Vol 85 (3) ◽  
pp. 740-749 ◽  
Author(s):  
Ole-Jacob Norum ◽  
Jon-Vidar Gaustad ◽  
Even Angell-Petersen ◽  
Einar K. Rofstad ◽  
Qian Peng ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Therapeutic Effect ◽  
Photochemical Internalization ◽  
Tumor Periphery
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