Generation of three-dimensional fluorescent spot with radially polarized Laguerre-Gaussian beams for STED microscopy imaging

2018 ◽  
Author(s):  
Shujing Liu ◽  
Mingyan Luo ◽  
Yanju Yang ◽  
Yingshun Xu ◽  
Dongqing Sun ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Gaussian Beams ◽  
Microscopy Imaging ◽  
Sted Microscopy ◽  
Radially Polarized ◽  
Fluorescent Spot

Three-dimensional isotropic STED microscopy generated by 4π focusing of a radially polarized vortex Laguerre–Gaussian beam

2020 ◽  
Vol 463 ◽  
pp. 125434 ◽  
Author(s):  
Mingyan Luo ◽  
Dongqing Sun ◽  
Yanju Yang ◽  
Shujing Liu ◽  
Jingwei Wu ◽  
...  
Keyword(s):  
Gaussian Beam ◽  
Three Dimensional ◽  
Sted Microscopy ◽  
Radially Polarized

Paraxial Propagation Properties of Radially Polarized Odd‐Pearcey Gaussian Beams in Free Space

2021 ◽  
pp. 2100055
Author(s):  
Mingyue Fang ◽  
Xiang Liao ◽  
Jiajia Zhao ◽  
Chuangjie Xu ◽  
Yixiao Zhang ◽  
...  
Keyword(s):  
Free Space ◽  
Gaussian Beams ◽  
Propagation Properties ◽  
Radially Polarized

scholarly journals Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?

2021 ◽  
Vol 22 (7) ◽  
pp. 3391
Author(s):  
Sylwia Grabska-Zielińska ◽  
Alina Sionkowska ◽  
Ewa Olewnik-Kruszkowska ◽  
Katarzyna Reczyńska ◽  
Elżbieta Pamuła
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Physicochemical Properties ◽  
Silk Fibroin ◽  
Three Dimensional ◽  
Microscopy Imaging ◽  
Maximum Deformation ◽  
One Step ◽  
Chitosan Blends ◽  
Fourier Transfer Infrared Spectroscopy

The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC). DAS was commercially available, while DAC was obtained by one-step synthesis. Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis. Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells. The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC. It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds. Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC. Additionally, scaffolds cross-linked with DAS exhibited higher biocompatibility than those cross-linked with DAC. However, the obtained results showed that both types of scaffolds can provide the support required in regenerative medicine and tissue engineering. The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects.

scholarly journals Three-Dimensional Cross-Sectional Light-Sheet Microscopy Imaging of the Inflamed Mouse Gut

2017 ◽  
Vol 153 (4) ◽  
pp. 898-900 ◽  
Author(s):  
Sebastian Zundler ◽  
Anika Klingberg ◽  
Daniela Schillinger ◽  
Sarah Fischer ◽  
Clemens Neufert ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Light Sheet ◽  
Cross Sectional ◽  
Microscopy Imaging ◽  
Light Sheet Microscopy

scholarly journals Plasmonic interference modulation for broadband nanofocusing

Nanophotonics ◽  
2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Shaobo Li ◽  
Shuming Yang ◽  
Fei Wang ◽  
Qiang Liu ◽  
Biyao Cheng ◽  
...  
Keyword(s):  
Near Field ◽  
Three Dimensional ◽  
Field Enhancement ◽  
Incident Beam ◽  
Plasmonic Mode ◽  
Resonant Structures ◽  
Interference Modulation ◽  
Near Field Imaging ◽  
Linearly Polarized ◽  
Radially Polarized

Abstract Metallic plasmonic probes have been successfully applied in near-field imaging, nanolithography, and Raman enhanced spectroscopy because of their ability to squeeze light into nanoscale and provide significant electric field enhancement. Most of these probes rely on nanometric alignment of incident beam and resonant structures with limited spectral bandwidth. This paper proposes and experimentally demonstrates an asymmetric fiber tip for broadband interference nanofocusing within its full optical wavelengths (500–800 nm) at the nanotip with 10 nm apex. The asymmetric geometry consisting of two semicircular slits rotates plasmonic polarization and converts the linearly polarized plasmonic mode to the radially polarized plasmonic mode when the linearly polarized beam couples to the optical fiber. The three-dimensional plasmonic modulation induces circumference interference and nanofocus of surface plasmons, which is significantly different from the nanofocusing through plasmon propagation and plasmon evolution. The plasmonic interference modulation provides fundamental insights into the plasmon engineering and has important applications in plasmon nanophotonic technologies.

Three dimensional live-cell STED microscopy at increased depth using a water immersion objective

2018 ◽  
Vol 89 (5) ◽  
pp. 053701 ◽  
Author(s):  
Jörn Heine ◽  
Christian A. Wurm ◽  
Jan Keller-Findeisen ◽  
Andreas Schönle ◽  
Benjamin Harke ◽  
...  
Keyword(s):  
Water Immersion ◽  
Three Dimensional ◽  
Live Cell ◽  
Sted Microscopy ◽  
Water Immersion Objective

Design of Three-Dimensional Superresolution Diffractive Optical Elements for Radially Polarized Beam

2010 ◽  
Vol 30 (11) ◽  
pp. 3295-3299 ◽  
Author(s):  
程侃 Cheng Kan ◽  
谭峭峰 Tan Qiaofeng ◽  
周哲海 Zhou Zhehai ◽  
金国藩 Jin Guofan
Keyword(s):  
Three Dimensional ◽  
Diffractive Optical Elements ◽  
Optical Elements ◽  
Radially Polarized Beam ◽  
Polarized Beam ◽  
Radially Polarized
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