High speed large-field-of-view scanning microscopy imaging technology and system implementation

2018 ◽  
Author(s):  
Liang Shan ◽  
Heng Mao ◽  
Muyue Zhai ◽  
Shuxiang Dong ◽  
Louis Tao
Keyword(s):  
High Speed ◽  
Field Of View ◽  
Scanning Microscopy ◽  
Large Field ◽  
System Implementation ◽  
Microscopy Imaging ◽  
Imaging Technology

scholarly journals A Stereo-Vision System for Measuring the Ram Speed of Steam Hammers in an Environment with a Large Field of View and Strong Vibrations

Sensors ◽  
2019 ◽  
Vol 19 (5) ◽  
pp. 996 ◽  
Author(s):  
Ran Chen ◽  
Zhongwei Li ◽  
Kai Zhong ◽  
Xingjian Liu ◽  
Yonghui Wu ◽  
...  
Keyword(s):  
Stereo Vision ◽  
High Speed ◽  
Vision System ◽  
Calibration Method ◽  
Bundle Adjustment ◽  
Field Of View ◽  
Large Field ◽  
Practical Applications ◽  
Accuracy Of Measurements ◽  
Ram Speed

The ram speed of a steam hammer is an important parameter that directly affects the forming performance of forgers. This parameter must be monitored regularly in practical applications in industry. Because of the complex and dangerous industrial environment of forging equipment, non-contact measurement methods, such as stereo vision, might be optimal. However, in actual application, the field of view (FOV) required to measure the steam hammer is extremely large, with a value of 2–3 m, and heavy steam hammer, at high-speed, usually causes a strong vibration. These two factors combine to sacrifice the accuracy of measurements, and can even cause the failure of measurements. To solve these issues, a bundle-adjustment-principle-based system calibration method is proposed to realize high-accuracy calibration for a large FOV, which can obtain accurate calibration results when the calibration target is not precisely manufactured. To decrease the influence of strong vibration, a stationary world coordinate system was built, and the external parameters were recalibrated during the entire measurement process. The accuracy and effectiveness of the proposed technique were verified by an experiment to measure the ram speed of a counterblow steam hammer in a die forging device.

scholarly journals Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry

2020 ◽  
Author(s):  
Che-Hang Yu ◽  
Jeffrey N. Stirman ◽  
Yiyi Yu ◽  
Riichiro Hira ◽  
Spencer L. Smith
Keyword(s):  
Adaptive Optics ◽  
Calcium Imaging ◽  
High Speed ◽  
Optical Design ◽  
Neural Circuitry ◽  
Brain Regions ◽  
Field Of View ◽  
Large Field ◽  
Two Photon ◽  
Subcellular Resolution

AbstractImaging the activity of neurons that are widely distributed across brain regions deep in scattering tissue at high speed remains challenging. Here, we introduce an open-source system with Dual Independent Enhanced Scan Engines for Large Field-of-view Two-Photon imaging (Diesel2p). Combining novel optical design, adaptive optics, and temporal multiplexing, the system offers subcellular resolution over a large field-of-view (∼ 25 mm2) with independent scan engines. We demonstrate the flexibility and various use cases of this system for calcium imaging of neurons in the living brain.

scholarly journals High-speed and Large Field-of-view Imaging via X-ray Fly-scan Ptychography

2019 ◽  
Vol 25 (S2) ◽  
pp. 46-47
Author(s):  
Yi Jiang ◽  
Junjing Deng ◽  
Jeffrey A. Klug ◽  
Yudong Yao ◽  
Curt Preissner ◽  
...  
Keyword(s):  
High Speed ◽  
Field Of View ◽  
Large Field ◽  
X Ray

Raster Adaptive Optics for Video Rate Laser Scanning Microscopy with Large Field of View Correction

2020 ◽  
Author(s):  
Yongxiao Li ◽  
Yean J. Lim ◽  
Qiongkai Xu ◽  
Lynette Beattie ◽  
Elizabeth E. Gardiner ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Field Of View ◽  
Scanning Microscopy ◽  
Large Field

scholarly journals High resolution, high speed, long working distance, large field of view confocal fluorescence microscope

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Shaun Pacheco ◽  
Chengliang Wang ◽  
Monica K. Chawla ◽  
Minhkhoi Nguyen ◽  
Brend K. Baggett ◽  
...  
Keyword(s):  
High Resolution ◽  
High Speed ◽  
Fluorescence Microscope ◽  
Field Of View ◽  
Large Field ◽  
Confocal Fluorescence ◽  
Working Distance

Spectral Microscopy Imaging System for High-Resolution and High-Speed Imaging of Fuel Sprays

2019 ◽  
Author(s):  
Kenneth F. Maassen ◽  
Farzad Poursadegh ◽  
Caroline L. Genzale
Keyword(s):  
High Resolution ◽  
High Speed ◽  
High Pressures ◽  
High Efficiency ◽  
Direct Injection ◽  
Field Of View ◽  
Resolving Power ◽  
Microscopy Imaging ◽  
Fuel Sprays ◽  
Primary Breakup

Abstract Modern high-efficiency engines utilize direct injection for charge preparation at extremely high pressures. At these conditions the scales of atomization become challenging to measure, as primary breakup occurs on the micrometer and nanosecond scales. As such, fuel sprays at these conditions have proven difficult to study via direct imaging. While high-speed cameras now exist that can shutter at tens to hundreds of nanoseconds, and long-range microscopes can be coupled to these cameras to provide high resolution images, the resolving power of these systems is typically limited by pixel size and field of view. The large pixel sizes make the realization of the diffraction-limited optical resolution quite challenging. On the other hand, limited data throughput under high repetition rate operation limits the field of view (FOV) due to reduced sensor area. Therefore, a novel measurement technique is critical to study fuel spray formation at engine-relevant conditions. In this work, we demonstrate a new high-resolution imaging technique, Spectral Microscopy, which aims to realize diffraction-limited imaging at effective framerates sufficient for capturing primary breakup in engine-relevant sprays. A spectral microscopy system utilizing a consumer-grade DSLR allows for significantly wider FOV with improved resolving power compared to high-speed cameras. Temporal shuttering is accomplished via separate and independently triggered back illumination sources, with wavelengths selected to overlap with the detection bands of the camera sensor’s RGB filter array. The RGB detection channels act as filters to capture independently timed red, green, and blue light pulses, enabling the capture of a three consecutive images at effective framerates exceeding 20 Million fps. To optimize system performance, a backlit illumination system is designed to maximize light throughput, a multi-lens setup is created, and an image processing algorithm is demonstrated that formulates a three-frame image from the camera sensor. The system capabilities are then demonstrated by imaging engine relevant diesel sprays. The spectral microscopy system detailed in this paper allows for micron-scale feature recognition at framerates exceeding 20 Million fps, thus expanding the capability for experimental research on primary breakup in fuel sprays for modern direct-injection engines.

Design of Image Transmission System for Multi Channel Panchromatic TDICCD Camera with Large Field of View

2013 ◽  
Vol 380-384 ◽  
pp. 3758-3761 ◽  
Author(s):  
Long Xu Jin ◽  
Jin Li ◽  
Xian Peng Hao
Keyword(s):  
Time Delay ◽  
High Speed ◽  
Image Transmission ◽  
Transmission System ◽  
Field Of View ◽  
Large Field ◽  
Single Chip ◽  
Charge Coupled Devices ◽  
Data Volume ◽  
Ccd Image

In order to satisfy the transmission of mass data volume of on-board time delay integration charge coupled devices (TDICCD) image, a high speed image transmission system of space panchromatic TDICCD camera with a large field of view is designed in this paper. A 4-channel parallel high speed transmission system for 16-channel CCD image was designed. A high speed serializer TLK2711 is applied to send and receive multi channel TDICCD images. A single chip TLK2711 can processing 4 channels image. Experimental results show that the designed transmission system can reliably work. The designed transmission system can meet the application for space TDICCD camera with a large field of view.

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