DNA sequencing using four-color capillary array electrophoresis and energy-transfer primers

1996 ◽  
Author(s):  
Indu Kheterpal ◽  
Jingyue Ju ◽  
Arun Radhakrishnan ◽  
Gabriel S. Brandt ◽  
Charles L. Ginther ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Dna Sequencing ◽  
Capillary Array Electrophoresis ◽  
Capillary Array

Peer Reviewed: Capillary Array Electrophoresis DNA Sequencing.

1999 ◽  
Vol 71 (1) ◽  
pp. 31A-37A ◽  
Author(s):  
Indu Kheterpal ◽  
Richard A. Mathies
Keyword(s):  
Dna Sequencing ◽  
Capillary Array Electrophoresis ◽  
Capillary Array

Ultra-high throughput rotary capillary array electrophoresis scanner for fluorescent DNA sequencing and analysis

1999 ◽  
Vol 20 (7) ◽  
pp. 1508-1517 ◽  
Author(s):  
James R. Scherer ◽  
Indu Kheterpal ◽  
Arun Radhakrishnan ◽  
William W. Ja ◽  
Richard A. Mathies
Keyword(s):  
Dna Sequencing ◽  
High Throughput ◽  
Capillary Array Electrophoresis ◽  
Capillary Array

Rapid Sizing of Short Tandem Repeat Alleles Using Capillary Array Electrophoresis and Energy-Transfer Fluorescent Primers

1995 ◽  
Vol 67 (7) ◽  
pp. 1197-1203 ◽  
Author(s):  
Yiwen. Wang ◽  
Jingyue. Ju ◽  
Brooke A. Carpenter ◽  
Jeanette M. Atherton ◽  
George F. Sensabaugh ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Capillary Array Electrophoresis ◽  
Capillary Array ◽  
Fluorescent Primers ◽  
Short Tandem

scholarly journals Genotyping Energy-Transfer-Cassette-labeled Short-Tandem-Repeat Amplicons with Capillary Array Electrophoresis Microchannel Plates

2001 ◽  
Vol 47 (9) ◽  
pp. 1614-1621 ◽  
Author(s):  
Igor L Medintz ◽  
Lorenzo Berti ◽  
Charles A Emrich ◽  
Jennifer Tom ◽  
James R Scherer ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Genetic Analysis ◽  
High Throughput ◽  
High Performance ◽  
Microchannel Plate ◽  
Str Analysis ◽  
Capillary Array Electrophoresis ◽  
Microchannel Plates ◽  
Capillary Array ◽  
Short Tandem

Abstract Background: Genetic analysis of microsatellite DNA is a powerful tool used in linkage analysis, gene mapping, and clinical diagnosis. To address the expanding needs of studies of short tandem repeats (STRs), we demonstrated high-performance STR analysis on a high-throughput microchannel plate-based platform. Methods: Energy-transfer-cassette-labeled STR amplicons were separated and typed on a microfabricated 96-channel radial capillary array electrophoresis (CAE) microchannel plate system. Four-color detection was accomplished with a laser-excited confocal fluorescence rotary scanner. Results: Multiplex STR analysis with single base-pair resolution was demonstrated on denaturing polyacrylamide gel media. The high-throughput multiplex capabilities of this genetic analysis platform were demonstrated by the simultaneous separation of STR amplicons representing 122 samples in ninety-six 5.5-cm-long channels in <8 min. Sizing values obtained for these amplicons on the CAE microchannel plate were comparable to those measured on a conventional commercial CAE instrument and exhibit <1% sizing variance. Conclusions: Energy-transfer-cassette labeling and microfabricated CAE microchannel plates allow high-performance multiplex STR analyses.

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