Super-resolution mapping of the local density of states with single-molecule and fluorescence lifetime imaging microscopy (Conference Presentation)

2018 ◽  
Author(s):  
Ignacio Izeddin ◽  
Dorian Bouchet ◽  
Jules Scholler ◽  
Valentina Krachmalnicoff
Keyword(s):  
Density Of States ◽  
Single Molecule ◽  
Local Density ◽  
Super Resolution ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Local Density Of States ◽  
Lifetime Imaging ◽  
Resolution Mapping ◽  
Super Resolution Mapping

scholarly journals Multiphoton fluorescence lifetime imaging microscopy (FLIM) and super-resolution fluorescence imaging with a supramolecular biopolymer for the controlled tagging of polysaccharides

Nanoscale ◽  
2019 ◽  
Vol 11 (19) ◽  
pp. 9498-9507 ◽  
Author(s):  
Haobo Ge ◽  
Fernando Cortezon-Tamarit ◽  
Hui-Chen Wang ◽  
Adam C. Sedgwick ◽  
Rory L. Arrowsmith ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Fluorescence Lifetime ◽  
Super Resolution ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Boronate Ester ◽  
Lifetime Imaging ◽  
In Cells

A new coumarin-appended boronate ester for fluorogenic imaging which binds polysaccharides in solution and in cells.

scholarly journals In situ real-time monitoring the mechanism of self-assembly of short peptide supramolecular polymers

2021 ◽  
Author(s):  
Mari C. Mañas-Torres ◽  
Cristina Gila-Vilchez ◽  
Juan Antonio Gonzalez Vera ◽  
Francisco Conejero-Lara ◽  
Victor Blanco ◽  
...  
Keyword(s):  
Single Molecule ◽  
Fluorescence Lifetime ◽  
Self Assembly ◽  
Correlation Spectroscopy ◽  
Supramolecular Polymers ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Single Molecule Fluorescence ◽  
Lifetime Imaging

Making use of the combination of multiparametric Fluorescence Lifetime Imaging Microscopy (FLIM) and single-molecule Fluorescence Lifetime Correlation Spectroscopy (FLCS), we have been able to study early stages of Fluorenylmethyloxycarbonyl-diphenylalanine (Fmoc-FF)...

scholarly journals Super-resolution mapping of glutamate receptors in C. elegans by confocal correlated PALM

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Jeroen Vangindertael ◽  
Isabel Beets ◽  
Susana Rocha ◽  
Peter Dedecker ◽  
Liliane Schoofs ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Single Molecule ◽  
Genetic Regulation ◽  
Super Resolution ◽  
Receptor Expression ◽  
C Elegans ◽  
Resolution Mapping ◽  
Super Resolution Mapping ◽  
Receptor Clusters

Abstract Photoactivated localization microscopy (PALM) is a super-resolution imaging technique based on the detection and subsequent localization of single fluorescent molecules. PALM is therefore a powerful tool in resolving structures and putative interactions of biomolecules at the ultimate analytical detection limit. However, its limited imaging depth restricts PALM mostly to in vitro applications. Considering the additional need for anatomical context when imaging a multicellular organism, these limitations render the use of PALM in whole animals difficult. Here we integrated PALM with confocal microscopy for correlated imaging of the C. elegans nervous system, a technique we termed confocal correlated PALM (ccPALM). The neurons, lying below several tissue layers, could be visualized up to 10 μm deep inside the animal. By ccPALM, we visualized ionotropic glutamate receptor distributions in C. elegans with an accuracy of 20 nm, revealing super-resolution structure of receptor clusters that we mapped onto annotated neurons in the animal. Pivotal to our results was the TIRF-independent detection of single molecules, achieved by genetic regulation of labeled receptor expression and localization to effectively reduce the background fluorescence. By correlating PALM with confocal microscopy, this platform enables dissecting biological structures with single molecule resolution in the physiologically relevant context of whole animals.

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