In-situ SERS observation of selective molecule optical trapping

2018 ◽  
Author(s):  
Kei Murakoshi ◽  
Nobuaki Oyamada ◽  
Hiro Minamimoto
Keyword(s):  
Optical Trapping

In Situ Microscopic Observation on Surface Kinetics in Optical Trapping-Induced Crystal Growth: Step Formation, Wetting Transition, and Nonclassical Growth

2019 ◽  
Vol 19 (7) ◽  
pp. 4138-4150 ◽  
Author(s):  
Hiromasa Niinomi ◽  
Teruki Sugiyama ◽  
Toru Ujihara ◽  
Suxia Guo ◽  
Jun Nozawa ◽  
...  
Keyword(s):  
Crystal Growth ◽  
Microscopic Observation ◽  
Optical Trapping ◽  
Wetting Transition ◽  
Surface Kinetics ◽  
Growth Step

scholarly journals Optimization of Optical Trapping and Laser Interferometry in Biological Cells

2020 ◽  
Vol 10 (14) ◽  
pp. 4970
Author(s):  
Yujiro Sugino ◽  
Masahiro Ikenaga ◽  
Daisuke Mizuno
Keyword(s):  
Optical Trapping ◽  
Laser Interferometry ◽  
Living Cells ◽  
Colloidal Probe ◽  
Non Invasive ◽  
In Situ Calibration ◽  
The Stability ◽  
Sample Heterogeneity

Optical trapping and laser interferometry enable the non-invasive manipulation of colloids, which can be used to investigate the microscopic mechanics of surrounding media or bound macromolecules. For efficient trapping and precise tracking, the sample media must ideally be homogeneous and quiescent whereas such conditions are usually not satisfied in vivo in living cells. In order to investigate mechanics of the living-cell interior, we introduced (1) the in-situ calibration of optical trapping and laser interferometry, and (2) 3-D feedback control of a sample stage to stably track a colloidal particle. Investigating systematic errors that appear owing to sample heterogeneity and focal offsets of a trapping laser relative to the colloidal probe, we provide several important caveats for conducting precise optical micromanipulation in living cells. On the basis of this study, we further improved the performance of the techniques to be used in cells, by optimizing the position sensitivity of laser interferometry and the stability of the feedback simultaneously.

scholarly journals In situ control of root–bacteria interactions using optical trapping in transparent soil

2021 ◽  
Author(s):  
Sisi Ge ◽  
Kathryn M Wright ◽  
Sonia N Humphris ◽  
Lionel Xavier Dupuy ◽  
Michael P MacDonald
Keyword(s):  
Hair Cells ◽  
Optical Trapping ◽  
Cell Types ◽  
Bacterial Attachment ◽  
Bacterial Cells ◽  
Subsequent Infection ◽  
Detachment Rate ◽  
Root Surfaces ◽  
Root Hair Cells

Bacterial attachment on root surfaces is an important step preceding the colonisation or internalisation and subsequent infection of plants by pathogens. Unfortunately, bacterial attachment is not well understood because the phenomenon is difficult to observe. Here we assessed whether this limitation could be overcome using optical trapping approaches. We have developed a system based on counter-propagating beams and studied its ability to guide Pectobacterium atrosepticum (Pba) cells to different root cell types within the interstices of transparent soils. Bacterial cells were successfully trapped and guided to root hair cells, epidermis cells, border cells and tissues damaged by laser ablation. Finally, we used the system to quantify the bacterial cell detachment rate of Pba cells on root surfaces following reversible attachment. Optical trapping techniques could greatly enhance our ability to deterministically characterise mechanisms linked to attachment and formation of biofilms in the rhizosphere.

In Situ Single-Cell Surgery and Intracellular Organelle Manipulation Via Thermoplasmonics Combined Optical Trapping

Nano Letters ◽  
2021 ◽  
Author(s):  
Xiaoting Zhao ◽  
Yang Shi ◽  
Ting Pan ◽  
Dengyun Lu ◽  
Jianyun Xiong ◽  
...  
Keyword(s):  
Single Cell ◽  
Optical Trapping ◽  
Intracellular Organelle ◽  
Cell Surgery

scholarly journals In situ viscometry by optical trapping interferometry

2008 ◽  
Vol 93 (18) ◽  
pp. 184102 ◽  
Author(s):  
Camilo Guzmán ◽  
Henrik Flyvbjerg ◽  
Roland Köszali ◽  
Carole Ecoffet ◽  
László Forró ◽  
...  
Keyword(s):  
Optical Trapping

Rendez vous with Saturn's rings

1984 ◽  
Vol 75 ◽  
pp. 743-759 ◽  
Author(s):  
Kerry T. Nock
Keyword(s):  
Solar Energy ◽  
Electric Propulsion ◽  
Power Source ◽  
Propulsion System ◽  
Low Thrust ◽  
Ring Plane ◽  
The Earth ◽  
Total Impulse ◽  
Saturn's Rings

ABSTRACTA mission to rendezvous with the rings of Saturn is studied with regard to science rationale and instrumentation and engineering feasibility and design. Future detailedin situexploration of the rings of Saturn will require spacecraft systems with enormous propulsive capability. NASA is currently studying the critical technologies for just such a system, called Nuclear Electric Propulsion (NEP). Electric propulsion is the only technology which can effectively provide the required total impulse for this demanding mission. Furthermore, the power source must be nuclear because the solar energy reaching Saturn is only 1% of that at the Earth. An important aspect of this mission is the ability of the low thrust propulsion system to continuously boost the spacecraft above the ring plane as it spirals in toward Saturn, thus enabling scientific measurements of ring particles from only a few kilometers.

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