Growth of Solanum tuberosum plantlets in vitro under LED light sources

2019 ◽  
Author(s):  
Olga Nakonechnaya ◽  
Irina Gafitskaya ◽  
Ola Grishchenko ◽  
Yuri Kulchin ◽  
Victor Bulgakov ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Light Sources ◽  
Led Light

Impact of LED light sources on morphogenesis and levels of photosynthetic pigments in Gerbera jamesonii grown in vitro

2018 ◽  
Vol 59 (1) ◽  
pp. 115-123 ◽  
Author(s):  
Bożena Pawłowska ◽  
Marek Żupnik ◽  
Bożena Szewczyk-Taranek ◽  
Monika Cioć
Keyword(s):  
Photosynthetic Pigments ◽  
Light Sources ◽  
Gerbera Jamesonii ◽  
Led Light

Responses of Favorita Potato Plantlets Cultured in Vitro under Fluorescent and Light-Emitting Diode (LED) Light Sources

2019 ◽  
Vol 96 (4) ◽  
pp. 396-402 ◽  
Author(s):  
Lili Jiang ◽  
Ziquan Wang ◽  
Guanghui Jin ◽  
Dianqiu Lu ◽  
Xuezhan Li
Keyword(s):  
Light Emitting Diode ◽  
Light Sources ◽  
Light Emitting ◽  
Led Light ◽  
Potato Plantlets

scholarly journals The effect of led light sources with varied spectral composition on the in vitro rooting bility of garden strawberry (Fragaria × ananassa)

2019 ◽  
Vol 180 (1) ◽  
pp. 33-37 ◽  
Author(s):  
I. A. Byadovsky
Keyword(s):  
Red Light ◽  
Spectral Composition ◽  
Light Flux ◽  
Light Sources ◽  
Fragaria Ananassa ◽  
Fluorescent Lamp ◽  
Led Light ◽  
Rooting Ability ◽  
Strawberry Cultivars

Consideration is given to the possibility of using LED light sources with different ratios of red (650 - 660 nm), orange (600 - 610 nm) and blue (440 - 450 nm) light in the light flux in the rooting stage of garden strawberry (Fragaria×ananassa(Duch. ex Weston) Duch. ex Rozier) grownin vitro. The study covered a sizable diversity of strawberry cultivars (selected from thein vitrocollection): ‘Borovitskaya’, ‘Vityaz’, ‘Zenga Zengana’, ‘Khoney’, ‘Tsaritsa’, ‘Kubata’, ‘Dukat’, ‘Marmalada’, ‘Valenta’, ‘Rusich’, ‘Krasavitsa Zagorya’, ‘Neznakomka’ and ‘Roksana’, thus ensuring quite diversified results. It was established that the use of LED sources with an increased content of red light in the light flux increased the rooting percentage (1.15-1.25 times) and the number of roots per one microcutting (1.29-1.77 times) inin vitroculture, as compared with fluorescent lamp lighting. It was observed that with different spectral compositions of light the rooting ability of microcuttings was cultivar-specific.

scholarly journals Red and Blue Light-Emitting Diodes Significantly Improve in vitro Tuberization of Potato (Solanum tuberosum L.)

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Robin Kumar Pundir ◽  
Abhishek Pathak ◽  
Devanshi Chandel Upadhyaya ◽  
Annamalai Muthusamy ◽  
Chandrama Prakash Upadhyaya
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Sensory System ◽  
Nodal Segments ◽  
Extrinsic Factors ◽  
Light Emitting ◽  
Regulatory Pathways ◽  
Blue Led ◽  
Led Light

Abstract The tuberization phenomenon in potatoes (Solanum tuberosum L.) is a highly synchronized morphophysiological process occurring on the underground stolons under the influence of various intrinsic and extrinsic factors. This involves the participation of the phytochrome sensory system, transport of sucrose from source to stolon, and several regulatory pathways including tuber-inducing hormone biosynthetic lipoxygenase (StLOX1) gene and major calcium-mediated signaling pathway genes (StCDPK and StCaM1). This study was aimed to explore the impacts of distinctive qualities of the light-emitting diode (LED) light with a specific wavelength on in vitro tuberization of potato. Single nodal segments of potato ‘Kufri Jyoti’ were incubated in vitro on the growth medium at a temperature of 22 ± 2 °C and exposed to various combinations of red (R) and blue (B) LED light. The results showed that the combination of 30% red + 70% blue LED light (R30B70) significantly shortened the tuber induction period, increased the number of tubers, and their yield compared with these parameters in the tissues exposed to the white light (W100). The induction of the in vitro tuberization correlated with the enhanced expression of the major tuberizing pathway genes, including the lipoxygenase (StLOX1), calmodulin (CaM1), and calcium-dependent protein kinase (StCDPK). The current study indicated that the combination of red and blue LED lights at R30B70 is the best spectrum for effective in vitro tuberization of potatoes.

scholarly journals Effect of led light on in vitro growth and developmentof Anoectochilus roxburghii

2018 ◽  
Vol 15 (1) ◽  
pp. 97-104
Author(s):  
Đỗ Thị Gấm ◽  
Chu Hoàng Hà Chu Hoàng Hà Chu Hoàng Hà ◽  
Phạm Bích Ngọc Phạm Bích Ngọc Phạm Bích Ngọc ◽  
Nguyễn Khắc Hưng ◽  
Phan Hồng Khôi ◽  
...  
Keyword(s):  
Growth And Development ◽  
Red Light ◽  
Light Condition ◽  
Luminous Intensity ◽  
Fluorescent Light ◽  
Light Sources ◽  
Plant Weight ◽  
Led Light ◽  
Anoectochilus Roxburghii

Recently, LED lighting technology has been developed fast and strongly. It has been widely used in many new fields. LEDs have been tested as an artificial light source during micropagation and tissue culture of various plants species as an alternative to traditional light sources to save energy and improve the efficiency of the culture process. In this study, effects of LED light at different wavelengths and luminous intensities on growth of Anoectochilus roxburghii's buds in vitro were investigated. Anoectochilus's buds were cultured under the different light conditions, such as LED in monochromatic red (R), LED in monochromatic green (B) , LED in the green and red light combining with warm white light (W) in different ratios (BRW 1, BRW2, BRW3 and BR). After 3 months of culture, the results showed LED B at strong luminous intensity (79 ± 3 μmol.m-2.s-1) inhibited the growth and development of Anoectochilus roxburghii. In contrast, BR LED with low luminous intensity (30 ± 1μmol.m-2.s-1) had made a positive effect on growth and development of Anoectochilus roxburghii. The plant height (5,88 cm), root length (1,33 cm), fresh plant weight (0,169 g/plant), leaf area (0,82 cm2), fresh leaf weight (18,33 mg/leaves) of plants in vitro were found higher than those of plants grown under T5 fluorescent lighting conditions (as control). Besides, chlorophyll a, chlorophyll b and total chlorophyll content of leaves (285,40 µg/g, 196,40 µg/g, 481,80 µg/g respectively) were higher than in the control and other versions of LED combine. The results also showed that the LED light with combined ratio of BR = 1: 4 at luminous intensity of 30 μmol.m-2.s-1 were suitable for the growth of Anoectochilus roxburghii. Therefore, BR LED (1:4) light condition should be replaced fluorescent light sources in vitro culture of Anoectochilus roxburghii in future.

Design of Solar-Powered LED Road Lighting System

2019 ◽  
pp. 75-85
Author(s):  
Canan Perdahci ◽  
Hamdi Ozkan
Keyword(s):  
High Pressure ◽  
Renewable Energy Sources ◽  
Junction Temperature ◽  
Mercury Vapour ◽  
Light Sources ◽  
Street Lighting ◽  
Led Light ◽  
Road Lighting ◽  
Lighting Systems ◽  
High Pressure Sodium Lamp

Turkey is rich in terms of renewable energy sources and, therefore, is now encouraging the use of sustainable clean lighting systems in road applications. High pressure sodium lamp is the most widely used type in main roads, but other types of lamps such as mercury vapour lamps or metal halide lamps can be utilized for street lighting. Since it enables energy and money saving, LED light technology has replaced high pressure sodium lamps nowadays. Once solar power system (PV) is integrated with LED lamp for street lighting, the amount of saving and local impact might be enriched. LEDs used as light sources in road lighting luminaires with rising lumen values, decreasing junction temperature, higher colour rendering efficiency, longer lifetime have become more efficient than many light sources with the latest developments. Since the structure of the luminaires in which the LED light sources are used differs from that of the conventional light sources, the optical, thermal and electrical design of the LED luminaires must be considered differently. Thus, this study concentrates upon design considerations and the operating principle of solarpowered LED road lighting luminaire in details. Also, a simple solar panel system was designed and the economical values obtained at the end of 20 years were compared when using the ongrid system and the off-grid system.

Respon Pemberian Aspirin dan Kinetin Terhadap Pembentukan Umbi Mikro Kentang (Solanum tuberosum L.) Secara In Vitro

2017 ◽  
Author(s):  
Yunita Prameswari ◽  
FNU Djenal ◽  
FNU Damanhuri
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L

Kebutuhan kentang yang semakin tinggi menyebabkan permintaan semakin meningkat. Rendahnya produksi kentang mengakibatkan berbagai upaya untuk peningkatan produksi terus dilakukan. Penggunaan metode kultur jaringan yaitu metode untuk mengisolasi bagian tanaman seperti protoplasma, sel, sekelompok sel, jaringan dan organ dalam kondisi aseptik, sehingga dapat diperbanyak dan beregenerasi menjadi tanaman utuh dapat dijadikan alternatif pemenuhan kebutuhan. Penelitian ini bertujuan untuk mengetahui kecepatan pembentukan umbi mikro kentang (Solanum Tuberosum L.) varietas granola kembang secarain vitro dengan menggunakan dua faktor dan 3 kali ulangan. Faktor pertama yaitu aspirin dengan tiga taraf (5,10,15) ppm. Faktor kedua yaitu kinetin dalam tiga taraf (8,10,12) ppm. Penelitian menggunakan seluruh propagul kentang yang berumur 30 hari setelah subkultur dan data yang didapat dianalisis menggunakan ANOVA. Hasil penelitian menunjukan bahwa interaksi aspirin dan kinetin tidak berpengaruh terhadap jumlah akar, kedinian umbi, dan bobot umbi. Interaksi perlakuan terbaik bagi pembentukan tunas yaitu A2K1 aspirin 10 ppm dan kinetin 8 ppm sedangkan Interaksi perlakuan terbaik pada parameter jumlah umbi yaituA3K2 aspirin 15 ppm dan kinetin 10.

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro)

2019 ◽  
Vol 9 (2) ◽  
pp. 91
Author(s):  
Ghea Dotulong ◽  
Stella Umboh ◽  
Johanis Pelealu
Keyword(s):  
Solanum Tuberosum ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Leaf Extract ◽  
Solanum Tuberosum L ◽  
In Vitro Toxicity ◽  
Potato Plants ◽  
Colony Diameter ◽  
In Vitro Toxicity Test

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro) Ghea Dotulong1*), Stella Umboh1), Johanis Pelealu1), 1) Program Studi Biologi, FMIPA Universitas Sam Ratulangi, Manado 95115*Email korespondensi: [email protected] Diterima 9 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 Abstrak Tanaman kentang (Solanum tuberosum L.) adalah salah satu tanaman hortikultura yang sering mengalami penurunan dari segi produksi dan produktivitasnya, akibat adanya serangan penyakit layu yang salah satunya disebabkan oleh Fusarium oxysporum. Tujuan penelitian ini adalah mengidentifikasi toksisitas beberapa fungisida nabati dalam mengendalikan penyakit Layu Fusarium (F. oxysporum) pada tanaman kentang (Solanum tuberosum L.) secara In Vitro. Metode Penelitian yang digunakan yaitu metode umpan beracun. Data dianalisis dengan Rancangan Acak Lengkap (RAL) dengan Analisis Varian (ANAVA) yang dilanjutkan dengan menggunakan metode BNT (Beda Nyata Terkecil). Hasil Penelitian, diperoleh nilai toksisitas fungisida nabati tertinggi yaitu pada ekstrak daun sirsak dengan nilai HR (69,44%), kategori berpengaruh, ditandai dengan diameter koloni 2,75 cm (100ppm) dan yang terendah toksisitasnya yaitu pada ekstrak daun jeruk purut dengan nilai HR (49,81%), kategori cukup berpengaruh ditandai dengan diameter koloni 3,75 cm (25ppm). Semakin tinggi konsentrasi yang diujikan maka semakin tinggi toksisitas dari fungisida nabati yang diberikan.Kata Kunci: fungisida nabati, Fusarium oxysporum, tanaman kentang, In Vitro Abstract Potato plants (Solanum tuberosum L.) is one of the horticulture plants which often decreases in terms of production and productivity, due to the attack of wilt, one of which is caused by Fusarium oxysporum. The purpose of this study was to determine the toxicity of several biofungicides in controlling Fusarium wilt (F. oxysporum) in potato plants (Solanum tuberosum L.) in Vitro. The research method used was the In Vitro method with the poison bait method. Data were analyzed by Completely Randomized Design with Variant Analysis (ANAVA), followed by the BNT method. The results showed that the highest biofungicide toxicity value was soursop leaf extract with HR values (69.44%), influential categories, characterized by colony diameter 2.75 cm (100ppm) and the lowest toxicity, namely in kaffir lime leaf extract with a value of HR (49.81%), quite influential category was characterized by colony diameter of 3.75 cm (25ppm). The higher the concentration tested, the higher the toxicity of the biofungicide given.Keywords: biofungicides, Fusarium oxysporum, Potato Plants, In Vitro.

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