Low light level quadriwave lateral shearing interferometer for in-situ wavefront sensing

2018 ◽  
Author(s):  
Brian Catanzaro ◽  
Benoit Wattellier ◽  
Robert A. Woodruff ◽  
Eliot Young ◽  
Michael J. Lewis ◽  
...  
Keyword(s):  
Light Level ◽  
Wavefront Sensing ◽  
Low Light ◽  
Lateral Shearing Interferometer ◽  
Lateral Shearing

In situ fine tuning of bendable soft x-ray mirrors using a lateral shearing interferometer

2013 ◽  
Vol 710 ◽  
pp. 82-86 ◽  
Author(s):  
Daniel J. Merthe ◽  
Kenneth A. Goldberg ◽  
Valeriy V. Yashchuk ◽  
Wayne R. McKinney ◽  
Richard Celestre ◽  
...  
Keyword(s):  
Fine Tuning ◽  
X Ray ◽  
Lateral Shearing Interferometer ◽  
Lateral Shearing

scholarly journals Prenatal Diagnosis of Parvovirus B19-Induced Hydrops Fetalis by Chemiluminescence In Situ Hybridization

1999 ◽  
Vol 37 (7) ◽  
pp. 2326-2329 ◽  
Author(s):  
M. Musiani ◽  
P. Pasini ◽  
M. Zerbini ◽  
G. Gentilomi ◽  
A. Roda ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
High Performance ◽  
Parvovirus B19 ◽  
Light Level ◽  
Optical Microscope ◽  
Hydrops Fetalis ◽  
Low Light ◽  
Luminescent Signal ◽  
Cord Blood Cells

Parvovirus B19 can be transmitted transplacentally from the infected mother to the fetus during pregnancy, and hydrops fetalis, abortion, or stillbirth can result. In our study we explored the use of chemiluminescence in situ hybridization to detect B19 DNA on cord blood cells, amniotic fluid cells, and pleuric fluid cells from several cases of hydrops fetalis. B19 DNA was detected by using digoxigenin-labeled probes immunoenzymatically visualized with the chemiluminescent adamantil-1,2-dioxetane phenyl phosphate substrate for alkaline phosphatase. The luminescent signal emitted from the hybridized probes was detected, analyzed, and measured with a high-performance, low-light-level imaging luminograph connected to an optical microscope and to a personal computer for the quantification and localization of the chemiluminescent emission inside individual cells.

Software pattern recognition applied to nanodiffraction patterns from a STEM instrument

1986 ◽  
Vol 44 ◽  
pp. 694-695
Author(s):  
G.Y. Fan ◽  
J.M. Cowley
Keyword(s):  
Image Processing ◽  
Pattern Recognition ◽  
Computer Software ◽  
Processing System ◽  
Light Level ◽  
Host Computer ◽  
Low Light ◽  
Image Processing System ◽  
Recent Developments ◽  
On Line

In recent developments, the ASU HB5 has been modified so that the timing, positioning, and scanning of the finely focused electron probe can be entirely controlled by a host computer. This made the asynchronized handshake possible between the HB5 STEM and the image processing system which consists of host computer (PDP 11/34), DeAnza image processor (IP 5000) which is interfaced with a low-light level TV camera, array processor (AP 400) and various peripheral devices. This greatly facilitates the pattern recognition technique initiated by Monosmith and Cowley. Software called NANHB5 is under development which, instead of employing a set of photo-diodes to detect strong spots on a TV screen, uses various software techniques including on-line fast Fourier transform (FFT) to recognize patterns of greater complexity, taking advantage of the sophistication of our image processing system and the flexibility of computer software.

Low-light-level three-dimensional video microscope with long working distance objective lenses

1994 ◽  
Vol 52 ◽  
pp. 226-227
Author(s):  
W. Lin ◽  
J. Gregorio ◽  
T.J. Holmes ◽  
D. H. Szarowski ◽  
J.N. Turner
Keyword(s):  
Three Dimensional ◽  
Light Level ◽  
Stereo Pair ◽  
Light Illumination ◽  
Initial Image ◽  
Low Light ◽  
Image Recording ◽  
Depth Discrimination ◽  
Video Microscope ◽  
Working Distance

A low-light level video microscope with long working distance objective lenses has been built as part of our integrated three-dimensional (3-D) light microscopy workstation (Fig. 1). It allows the observation of living specimens under sufficiently low light illumination that no significant photobleaching or alternation of specimen physiology is produced. The improved image quality, depth discrimination and 3-D reconstruction provides a versatile intermediate resolution system that replaces the commonly used dissection microscope for initial image recording and positioning of microelectrodes for neurobiology. A 3-D image is displayed on-line to guide the execution of complex experiments. An image composed of 40 optical sections requires 7 minutes to process and display a stereo pair.The low-light level video microscope utilizes long working distance objective lenses from Mitutoyo (10X, 0.28NA, 37 mm working distance; 20X, 0.42NA, 20 mm working distance; 50X, 0.42NA, 20 mm working distance). They provide enough working distance to allow the placement of microelectrodes in the specimen.

Theory and experiment study on low-light-level (LLL) image by topology mode filters

1998 ◽  
Author(s):  
Lianfa Bai ◽  
Qian Chen ◽  
Dekui Yin ◽  
Baomin Zhang
Keyword(s):  
Light Level ◽  
Experiment Study ◽  
Low Light ◽  
Theory And Experiment

Photoinhibition of DCMU-Enhanced Fluorescence in Lake Ontario Phytoplankton

1987 ◽  
Vol 44 (12) ◽  
pp. 2144-2154 ◽  
Author(s):  
M. Putt ◽  
G. P. Harris ◽  
R. L. Cuhel
Keyword(s):  
Vertical Mixing ◽  
Natural Populations ◽  
Bright Light ◽  
Euphotic Zone ◽  
Lake Ontario ◽  
Enhanced Fluorescence ◽  
Low Light ◽  
Light Levels ◽  
Phosphorus Status

Measurement of 1-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) enhanced fluorescence (FDCMU) suggested that photoinhibition of photosynthesis was frequently an artifact of in situ bottle incubations in Lake Ontario phytoplankton. In a seasonal study, FDCMU of all populations was depressed by bright light in an incubator. However, when the euphotic zone did not exceed the depth of the mixed layer, vertical transport of phytoplankton into either low-light or dark regions apparently allowed reversal of photoinhibition of FDCMU. Advantages of FDCMU as a bioassay of vertical mixing include rapidity of response time, ease of measurement in the field, and insensitivity of this parameter to changes in phosphorus status of the population. Because of seasonal changes in the photoadaptive response of natural populations, the rate constants and threshold light levels required to cause the response must be determined at each use if the method is to be quantitative.

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