Illuminating gene expression dynamics by optogenetics

2018 ◽  
Author(s):  
Akihiro Isomura
Keyword(s):  
Gene Expression ◽  
Gene Expression Dynamics

scholarly journals Genome‐wide gene expression dynamics of the fungal pathogen D othistroma septosporum throughout its infection cycle of the gymnosperm host P inus radiata

2015 ◽  
Vol 17 (2) ◽  
pp. 210-224 ◽  
Author(s):  
Rosie E. Bradshaw ◽  
Yanan Guo ◽  
Andre D. Sim ◽  
M. Shahjahan Kabir ◽  
Pranav Chettri ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fungal Pathogen ◽  
Infection Cycle ◽  
Genome Wide ◽  
Gene Expression Dynamics ◽  
Genome Wide Gene Expression

scholarly journals How to cluster gene expression dynamics in response to environmental signals

2011 ◽  
Vol 13 (2) ◽  
pp. 162-174 ◽  
Author(s):  
Y. Wang ◽  
M. Xu ◽  
Z. Wang ◽  
M. Tao ◽  
J. Zhu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Environmental Signals ◽  
Gene Expression Dynamics ◽  
Cluster Gene

Gene expression dynamics

2003 ◽  
Vol 57 (3-4) ◽  
pp. 180 ◽  
Author(s):  
M Dundr ◽  
T Misteli
Keyword(s):  
Gene Expression ◽  
Gene Expression Dynamics

scholarly journals A Novel Triple-Fluorescent HCMV Strain Reveals Gene Expression Dynamics and Anti-Herpesviral Drug Mechanisms

2021 ◽  
Vol 10 ◽  
Author(s):  
Ulfert Rand ◽  
Tobias Kubsch ◽  
Bahram Kasmapour ◽  
Luka Cicin-Sain
Keyword(s):  
Gene Expression ◽  
Hcmv Infection ◽  
Viral Fitness ◽  
High Temporal Resolution ◽  
Detection Techniques ◽  
Drug Candidates ◽  
New Drug ◽  
Gene Expression Dynamics ◽  
Identification And Characterization

Human Cytomegalovirus (HCMV) infection may result in severe outcomes in immunocompromised individuals such as AIDS patients, transplant recipients, and neonates. To date, no vaccines are available and there are only few drugs for anti-HCMV therapy. Adverse effects and the continuous emergence of drug-resistance strains require the identification of new drug candidates in the near future. Identification and characterization of such compounds and biological factors requires sensitive and reliable detection techniques of HCMV infection, gene expression and spread. In this work, we present and validate a novel concept for multi-reporter herpesviruses, identified through iterative testing of minimally invasive mutations. We integrated up to three fluorescence reporter genes into replication-competent HCMV strains, generating reporter HCMVs that allow the visualization of replication cycle stages of HCMV, namely the immediate early (IE), early (E), and late (L) phase. Fluorescent proteins with clearly distinguishable emission spectra were linked by 2A peptides to essential viral genes, allowing bicistronic expression of the viral and the fluorescent protein without major effects on viral fitness. By using this triple color reporter HCMV, we monitored gene expression dynamics of the IE, E, and L genes by measuring the fluorescent signal of the viral gene-associated fluorophores within infected cell populations and at high temporal resolution. We demonstrate distinct inhibitory profiles of foscarnet, fomivirsen, phosphonoacetic acid, ganciclovir, and letermovir reflecting their mode-of-action. In conclusion, our data argues that this experimental approach allows the identification and characterization of new drug candidates in a single step.

scholarly journals An ERK5-KLF2 Signalling Module Regulates Early Embryonic Gene Expression Dynamics and Stem Cell Rejuvenation

2021 ◽  
Author(s):  
Helen A. Brown ◽  
Charles A. C. Williams ◽  
Houjiang Zhou ◽  
Diana Rios-Szwed ◽  
Rosalia Fernandez-Alonso ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cell ◽  
Gene Expression Dynamics
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