A treatment of light scattering by rough surfaces in terms of the three-dimensional transfer function

Author(s):  
C. J. R. Sheppard
Author(s):  
Joachim Frank

Cryo-electron microscopy combined with single-particle reconstruction techniques has allowed us to form a three-dimensional image of the Escherichia coli ribosome.In the interior, we observe strong density variations which may be attributed to the difference in scattering density between ribosomal RNA (rRNA) and protein. This identification can only be tentative, and lacks quantitation at this stage, because of the nature of image formation by bright field phase contrast. Apart from limiting the resolution, the contrast transfer function acts as a high-pass filter which produces edge enhancement effects that can explain at least part of the observed variations. As a step toward a more quantitative analysis, it is necessary to correct the transfer function in the low-spatial-frequency range. Unfortunately, it is in that range where Fourier components unrelated to elastic bright-field imaging are found, and a Wiener-filter type restoration would lead to incorrect results. Depending upon the thickness of the ice layer, a varying contribution to the Fourier components in the low-spatial-frequency range originates from an “inelastic dark field” image. The only prospect to obtain quantitatively interpretable images (i.e., which would allow discrimination between rRNA and protein by application of a density threshold set to the average RNA scattering density may therefore lie in the use of energy-filtering microscopes.


2003 ◽  
Author(s):  
Fernando Perez Quintian ◽  
Maria A. Rebollo ◽  
Ricardo G. Berlasso ◽  
Nestor G. Gaggioli

2008 ◽  
Vol 47 (7) ◽  
pp. 5576-5580 ◽  
Author(s):  
Passapong Wutimakun ◽  
Taichiro Mori ◽  
Hisashi Miyazaki ◽  
Yoichi Okamoto ◽  
Jun Morimoto ◽  
...  

Author(s):  
K. Farhang ◽  
A. Sepehri ◽  
D. Segalman ◽  
M. Starr

Energy dissipation in mechanical joints occurs as a result of micro-slip motion between contacting rough surfaces. An account of this phenomenon is especially challenging due to the vast differences in the length and time scale differences between the macro-mechanical structure and the micron-scale events at the joint interface. This paper considers the contact between two nominally flat surfaces containing micron-scale roughness. The rough surface interaction is viewed as a multi-sphere elastic interaction subject to a periodic tangential force. It combines the Mindlin’s formulation [1, 2] for the elastic interaction of two spheres with the Greenwood and Williamson’s [3] statistical approach for the contact of two nominally flat rough surfaces so as to develop a model for multi-sphere problem in which sphere radii, contact load and the number of spheres in contact can only be known in a statistical sense and not deterministically.


2006 ◽  
Vol 128 (4) ◽  
pp. 745-752 ◽  
Author(s):  
C. J. Hooke ◽  
K. Y. Li

Using modern EHL programs it is relatively simple to determine the pressures and clearances in rough EHL contacts. The pressures may then be used to calculate the subsurface stresses in the two contacting components. However, the results depend on the assumptions made about the fluid’s rheology. While it is possible to measure the clearances using interferometric techniques, measurement of either the pressures or stresses is extremely difficult. However it is these, rather than the clearances, that determine the life of the contact. In previous papers the authors have described how the inverse method may be used to validate the stress predictions for contacts with transverse roughness. This type of contact has fluid flow in only one plane and it remained necessary to check the results for more general rough surfaces where the flow is three-dimensional. Accordingly, the inverse method is extended, in this paper, to a situation where out-of-plane flow is significant. The paper describes the approach and presents some preliminary results for rolling contacts.


2009 ◽  
Vol 131 (2) ◽  
Author(s):  
Stephen T. McClain ◽  
Jason M. Brown

The discrete-element model for flows over rough surfaces was recently modified to predict drag and heat transfer for flow over randomly rough surfaces. However, the current form of the discrete-element model requires a blockage fraction and a roughness-element diameter distribution as a function of height to predict the drag and heat transfer of flow over a randomly rough surface. The requirement for a roughness-element diameter distribution at each height from the reference elevation has hindered the usefulness of the discrete-element model and inhibited its incorporation into a computational fluid dynamics (CFD) solver. To incorporate the discrete-element model into a CFD solver and to enable the discrete-element model to become a more useful engineering tool, the randomly rough surface characterization must be simplified. Methods for determining characteristic diameters for drag and heat transfer using complete three-dimensional surface measurements are presented. Drag and heat transfer predictions made using the model simplifications are compared to predictions made using the complete surface characterization and to experimental measurements for two randomly rough surfaces. Methods to use statistical surface information, as opposed to the complete three-dimensional surface measurements, to evaluate the characteristic dimensions of the roughness are also explored.


2013 ◽  
Vol 103 (4) ◽  
pp. 041106 ◽  
Author(s):  
Susanna Orlic ◽  
Fabian Bernstein ◽  
Christoph Kratz ◽  
Alexander Schlösser

2015 ◽  
Vol 5 (1) ◽  
pp. 20140065 ◽  
Author(s):  
Alexander E. Filippov ◽  
Stanislav N. Gorb

One of the important problems appearing in experimental realizations of artificial adhesives inspired by gecko foot hair is so-called clusterization. If an artificially produced structure is flexible enough to allow efficient contact with natural rough surfaces, after a few attachment–detachment cycles, the fibres of the structure tend to adhere one to another and form clusters. Normally, such clusters are much larger than original fibres and, because they are less flexible, form much worse adhesive contacts especially with the rough surfaces. Main problem here is that the forces responsible for the clusterization are the same intermolecular forces which attract fibres to fractal surface of the substrate. However, arrays of real gecko setae are much less susceptible to this problem. One of the possible reasons for this is that ends of the seta have more sophisticated non-uniformly distributed three-dimensional structure than that of existing artificial systems. In this paper, we simulated three-dimensional spatial geometry of non-uniformly distributed branches of nanofibres of the setal tip numerically, studied its attachment–detachment dynamics and discussed its advantages versus uniformly distributed geometry.


Sign in / Sign up

Export Citation Format

Share Document