Tissue welding for corneal wound suture with a CW 1.9-um diode laser: an in-vivo preliminary study

1996 ◽  
Author(s):  
Thomas Desmettre ◽  
Serge R. Mordon ◽  
Valerie A. Mitchell
Keyword(s):  
Diode Laser ◽  
Tissue Welding ◽  
Corneal Wound ◽  
Preliminary Study

Tissue welding with 980-nm diode laser system: preliminary study for determination of optimal parameters

2006 ◽  
Author(s):  
Zeynep D. Korkut ◽  
Haşim Ö. Tabakoglu ◽  
Özgüncem Bozkulak ◽  
Ayla A. Aksel ◽  
Murat Gulsoy
Keyword(s):  
Diode Laser ◽  
Laser System ◽  
Optimal Parameters ◽  
Tissue Welding ◽  
Preliminary Study

scholarly journals Partial Decellularization for Segmental Tracheal Scaffold Tissue Engineering: A Preliminary Study in Rabbits

Biomolecules ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 866
Author(s):  
Luong Huu Dang ◽  
Yuan Tseng ◽  
How Tseng ◽  
Shih-Han Hung
Keyword(s):  
Mechanical Stability ◽  
Vital Staining ◽  
Potential Method ◽  
Term Survival ◽  
Long Term Survival ◽  
Cartilage Cells ◽  
Preliminary Study ◽  
Epithelial Layers

In this study, we developed a new procedure for the rapid partial decellularization of the harvested trachea. Partial decellularization was performed using a combination of detergent and sonication to completely remove the epithelial layers outside of the cartilage ring. The post-decellularized tracheal segments were assessed with vital staining, which showed that the core cartilage cells remarkably remained intact while the cells outside of the cartilage were no longer viable. The ability of the decellularized tracheal segments to evade immune rejection was evaluated through heterotopic implantation of the segments into the chest muscle of rabbits without any immunosuppressive therapy, which demonstrated no evidence of severe rejection or tissue necrosis under H&E staining, as well as the mechanical stability under stress-pressure testing. Finally, orthotopic transplantation of partially decellularized trachea with no immunosuppression treatment resulted in 2 months of survival in two rabbits and one long-term survival (2 years) in one rabbit. Through evaluations of posttransplantation histology and endoscopy, we confirmed that our partial decellularization method could be a potential method of producing low-immunogenic cartilage scaffolds with viable, functional core cartilage cells that can achieve long-term survival after in vivo transplantation.

scholarly journals Feasibility of balloon-based endobiliary radiofrequency ablation under cholangioscopy guidance in a swine model

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tadahisa Inoue ◽  
Hiromu Kutsumi ◽  
Mayu Ibusuki ◽  
Masashi Yoneda
Keyword(s):  
Radiofrequency Ablation ◽  
Adverse Events ◽  
Swine Model ◽  
Biliary Strictures ◽  
Tissue Samples ◽  
Ablation Area ◽  
Mucosal Changes ◽  
The Mean ◽  
Preliminary Study

AbstractAlthough endobiliary radiofrequency ablation (RFA) has demonstrated considerable potential for the treatment of biliary strictures, conventional catheter RFA has several limitations. This study aimed to evaluate the feasibility of a novel cholangioscopy (CS)-guided balloon-based RFA procedure in vivo using a swine model. CS-guided balloon-RFA was performed under endoscopic retrograde cholangiography guidance at target temperatures of 60 ℃ or 70 ℃, which were maintained for 60 s. We evaluated the technical feasibility, adverse events, and histological effects associated with the procedure. Twelve sites were ablated in seven miniature pigs. The CS-guided balloon-RFA procedure was technically successful in all cases without any hindrance. Mucosal changes could be detected during RFA, and the ablation area was identified on CS. Necropsy was performed in four pigs on the same day as the procedure: the tissue samples showed coagulative necrosis, and the entire internal circumference of the bile duct was uniformly ablated. The mean lengths of the ablation area in the samples ablated at 60 °C and 70 °C were 20.64 and 22.18 mm, respectively, while the mean depths were 3.46 and 5.07 mm, respectively. The other three pigs were reared and euthanized and autopsied 35 days after the procedure. The site to be ablated had replaced the granulation tissue and fibrotic changes. No adverse events were observed in any case. CS-guided balloon-RFA appears to be a promising option for treating biliary strictures. This preliminary study could pave the way for the evaluation of this procedure in future human clinical trials.

In Vivo Laser Tissue Welding in the Rabbit Maxillary Sinus

2008 ◽  
Vol 22 (6) ◽  
pp. 625-628 ◽  
Author(s):  
Benjamin S. Bleier ◽  
James N. Palmer ◽  
Michael A. Gratton ◽  
Noam A. Cohen
Keyword(s):  
Intracranial Pressure ◽  
Maxillary Sinus ◽  
Skull Base ◽  
Thermal Injury ◽  
Surrounding Tissue ◽  
Csf Leak ◽  
Tissue Welding ◽  
Laser Tissue Welding ◽  
And Control

Background One of the challenges in the current expansion of endoscopic sinonasal surgery is the ability to adequately reconstruct the skull base. Laser tissue welding (LTW) uses laser energy coupled to a biological solder to produce tissue bonds with burst thresholds exceeding human intracranial pressure. This technology could be used to reduce the rate of postoperative cerebrospinal fluid (CSF) leak. We performed this study to determine whether LTW can create durable tissue bonds in sinonasal mucosa that support normal wound healing and produce minimal collateral thermal injury. Methods Bilateral maxillary sinus mucosal incisions were made in 20 New Zealand white rabbits and one side was repaired using LTW. Burst pressure thresholds were measured on postoperative days 0, 5, and 15 and were compared with control using a two- way ANOVA and a post hoc Tukey test. Welds were examined histologically for thermal injury, inflammation, and fibroplasia and graded on a 4-point scale by three blinded observers. Results The burst pressures of the LTW group were significantly higher than control on postoperative day 0 (120.85 mm Hg, N = 4, SD = 47.84 versus 7.85 mm Hg, N = 4, SD = 0.78), and day 5 (132.56 mm Hg, N = 8, SD = 24.02 versus 41.7 mm Hg, N = 8, SD = 7.2; p < 0.05). By postoperative day 15 there was no significant difference between LTW (169.64 mm Hg, N = 8, SD = 18.49) and control (160.84 mm Hg, N = 8, SD = 14.16) burst thresholds. There was no evidence of thermal injury to the surrounding tissue in any group as well as no difference between experimental group and control with respect to inflammation or fibroplasia. Conclusion This is the first in vivo study showing that LTW is capable of producing tissue bonds exceeding human intracranial pressure with negligible thermal injury in sinonasal tissue. Welding can be performed endoscopically using a fiberoptic cable and may be useful in CSF leak and skull base repair.

Diode laser and microvascular carotid anastomosis: A preliminary study

1993 ◽  
Vol 8 (1) ◽  
pp. 33-38 ◽  
Author(s):  
G. Godlewski ◽  
J. M. Frapier ◽  
B. De Balmann ◽  
H. Mouzayek ◽  
S. Rouy ◽  
...  
Keyword(s):  
Diode Laser ◽  
Preliminary Study

In vivo laser-induced interstitial thermotherapy of pig liver with a temperature-controlled diode laser and MRI correlation

2001 ◽  
Vol 29 (4) ◽  
pp. 374-378 ◽  
Author(s):  
Walter A. Wohlgemuth ◽  
G�nther Wamser ◽  
Thomas Reiss ◽  
Theodor Wagner ◽  
Klaus Bohndorf
Keyword(s):  
Diode Laser ◽  
Pig Liver ◽  
Temperature Controlled ◽  
Interstitial Thermotherapy

scholarly journals Darkfield orthogonal polarized spectral imaging for studying endovascular laser-tissue interactions in vivo-a preliminary study

2005 ◽  
Vol 13 (3) ◽  
pp. 702 ◽  
Author(s):  
Michal Heger ◽  
Johan F. Beek ◽  
Karin Stenback ◽  
Dirk J. Faber ◽  
Martin J.C. van Gemert ◽  
...  
Keyword(s):  
Spectral Imaging ◽  
Tissue Interactions ◽  
Preliminary Study

Preliminary study of in vivo autofluorescence of nasopharyngeal carcinoma and normal tissue

2000 ◽  
Vol 26 (5) ◽  
pp. 432-440 ◽  
Author(s):  
Jianan Y. Qu ◽  
Po Wing ◽  
Zhijian Huang ◽  
Dora Kwong ◽  
Jonathan Sham ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Normal Tissue ◽  
Preliminary Study
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