Laser confocal microscopy study of impacts from a CO2 industrial laser to select optimal modal distribution

1996 ◽  
Author(s):  
M. F. Creus
Keyword(s):  
Confocal Microscopy ◽  
Microscopy Study ◽  
Laser Confocal Microscopy

A KATP channel opener protects cardiomyocytes from Ca2+ waves: a laser confocal microscopy study

1996 ◽  
Vol 270 (4) ◽  
pp. H1384-H1389 ◽  
Author(s):  
J. R. Lopez ◽  
R. A. Ghanbari ◽  
A. Terzic
Keyword(s):  
Confocal Microscopy ◽  
Potassium Channel ◽  
Average Frequency ◽  
Microscopy Study ◽  
Cardiac Cells ◽  
Laser Confocal Microscopy ◽  
Acetoxymethyl Ester ◽  
Ventricular Cells ◽  
Channel Opening ◽  
K Concentration

Laser confocal microscopy was used to visualize intracellular spatiotemporal Ca2+ patterns in single guinea pig ventricular myocytes loaded with the Ca2+ indicator, fluo 3-acetoxymethyl ester (fluo 3-AM), and exposed to moderately elevated extracellular K+ to induce partial membrane depolarization. Analysis of K(+)-induced intracellular Ca2+ elevation revealed three distinct paradigms: 1) diffuse, nonoscillatory Ca2+ elevation across the myocyte; 2) localized Ca2+ elevation in anatomically restricted areas (Ca2+ sparks); and 3) regenerative frontal propagations of Ca2+ that traversed the length of the cell (Ca2+ waves). The first two patterns were more frequently observed when the extracellular K+ concentration was raised to 8 mM. Ca2+ waves became more common when extracellular K+ concentration was increased to 16 mM, suggesting that a minimum threshold of increase in intracellular Ca2+ is necessary for the organization of Ca2+ waves. The velocity of propagation was typically approximately 60 microns/s with an average frequency of one wave per second crossing at a given point in the cell. Wave propagation resulted in spatial and temporal oscillations in cytosolic and nuclear Ca2+ concentration. Treating cardiac cells with aprikalim, a potassium channel-opening drug, prevented 16 mM K+ (but not 32 mM K+) from inducing an increase in Ca2+ concentration and from generating Ca2+ waves. In cardiomyocytes treated with glyburide, a selective antagonist of ATP-sensitive K+ channels, aprikalim failed to prevent 16 mM K+ from inducing Ca2+ waves. In summary, moderate hyperkalemia induces distinct nonuniform form patterns of intracellular Ca2+ elevation in ventricular cells, which can be prevented by a potassium channel-opening drug through a glyburide-sensitive mechanism.

scholarly journals 3-D laser confocal microscopy study of the oxidation of NdFeB magnets in atmospheric conditions

2016 ◽  
Vol 378 ◽  
pp. 540-544 ◽  
Author(s):  
J.P. Meakin ◽  
J.D. Speight ◽  
R.S. Sheridan ◽  
A. Bradshaw ◽  
I.R. Harris ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Microscopy Study ◽  
Atmospheric Conditions ◽  
Laser Confocal Microscopy ◽  
Ndfeb Magnets

Confocal microscopy study of membrane organelles of the skeletal muscle fiber in the process of Zenker’s (spreading) necrosis

2007 ◽  
Vol 1 (2) ◽  
pp. 183-190 ◽  
Author(s):  
S. A. Krolenko ◽  
S. Ya. Adamyan ◽  
T. N. Belyaeva ◽  
T. P. Mozhenok ◽  
A. V. Salova
Keyword(s):  
Skeletal Muscle ◽  
Confocal Microscopy ◽  
Muscle Fiber ◽  
Microscopy Study ◽  
Skeletal Muscle Fiber

Conjunctival findings in hyperbaric and low-tension glaucoma: an in vivo confocal microscopy study

2011 ◽  
Vol 90 (2) ◽  
pp. e132-e137 ◽  
Author(s):  
Luca Agnifili ◽  
Paolo Carpineto ◽  
Vincenzo Fasanella ◽  
Rodolfo Mastropasqua ◽  
Antonio Zappacosta ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Microscopy Study ◽  
In Vivo Confocal Microscopy ◽  
Low Tension Glaucoma ◽  
Low Tension

Confocal microscopy study of decorin binding to collagen fibrils

2008 ◽  
Vol 27 ◽  
pp. 56
Author(s):  
Elena Makareeva ◽  
Mary B. Sutter ◽  
Angela M. DeRidder ◽  
Antonella Forlino ◽  
Antonio Rossi ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Microscopy Study ◽  
Collagen Fibrils

Evaluation of Corneal Microstructure in Keratoconus: A Confocal Microscopy Study

2013 ◽  
Vol 156 (5) ◽  
pp. 885-893.e2 ◽  
Author(s):  
Engin Bilge Ozgurhan ◽  
Necip Kara ◽  
Aydin Yildirim ◽  
Ercument Bozkurt ◽  
Hasim Uslu ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Microscopy Study

scholarly journals The Effects of Diuretics on Intracellular Ca2+ Dynamics of Arteriole Smooth Muscles as Revealed by Laser Confocal Microscopy

2009 ◽  
Vol 42 (4) ◽  
pp. 121-128 ◽  
Author(s):  
Yasunori Tamagawa ◽  
Tomoyuki Saino ◽  
Makoto Matsuura ◽  
Yoh-ichi Satoh
Keyword(s):  
Confocal Microscopy ◽  
Smooth Muscles ◽  
Laser Confocal Microscopy
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