Cell patterns for biosensors manufactured with laser tweezers

1996 ◽  
Author(s):  
Joachim Renken ◽  
Stefan Seeger
Keyword(s):  
Laser Tweezers ◽  
Cell Patterns

EXPRESS: Characterization of Drug Resistance in Chronic Myeloid Leukemia Cells Based on Laser Tweezers Raman Spectroscopy

2021 ◽  
pp. 000370282110245
Author(s):  
Qian Zhang ◽  
Minlu Ye ◽  
Lingyan Wang ◽  
Dongmei Jiang ◽  
Shuting Yao ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Chronic Myeloid Leukemia ◽  
Myeloid Leukemia ◽  
K562 Cells ◽  
Laser Tweezers ◽  
Label Free ◽  
Multivariate Statistical ◽  
Analytical Strategy ◽  
Specificity And Sensitivity ◽  
Acid Protein

Multidrug resistance (MDR) is highly associated with poor prognosis of chronic myeloid leukemia (CML). This work aims to explore whether the laser tweezers Raman spectroscopy (LTRS) could be practical in separating adriamycin (ADR) resistance CML cells K562/ADR from its parental cells K562, and to explore the potential mechanisms. Detection of LTRS initially reflected the spectral differences caused by chemoresistance including bands assigned to carbohydrates, amino acid, protein, lipids and nucleic acid. In addition, principal components analysis (PCA) as well as the classification and regression trees (CRT) algorithms showed that the specificity and sensitivity were above 90%. Moreover, the band data-based CRT model and receiver operating characteristic (ROC) curve further determined some important bands and band intensity ratios to be reliable indexes in discriminating K562 chemoresistance status. Finally, we highlighted three metabolism pathways correlated with chemoresistance. This work demonstrates that the label-free LTRS analysis combined with multivariate statistical analyses have great potential to be a novel analytical strategy at the single-cell level for rapid evaluation the chemoresistance status of K562 cells.

scholarly journals Effect of boundaries on grid cell patterns

2020 ◽  
Vol 2 (4) ◽  
Author(s):  
Mauro M. Monsalve-Mercado ◽  
Christian Leibold
Keyword(s):  
Grid Cell ◽  
Cell Patterns

Simple and non-toxic fabrication of poly(vinyl alcohol)-patterned polymer surface for the formation of cell patterns

2014 ◽  
Vol 316 ◽  
pp. 179-186 ◽  
Author(s):  
In-Tae Hwang ◽  
Yu-Ran Jin ◽  
Min-Suk Oh ◽  
Chan-Hee Jung ◽  
Jae-Hak Choi
Keyword(s):  
Vinyl Alcohol ◽  
Polymer Surface ◽  
Poly Vinyl Alcohol ◽  
Cell Patterns

scholarly journals P57 A new technique for polar body biopsy with laser tweezers and femtosecond laser zona pellucida drilling

2010 ◽  
Vol 20 ◽  
pp. S41-S42
Author(s):  
S.A. Sergeev ◽  
M.M. Rakityanskiy ◽  
Y.V. Khramova ◽  
M.L. Semenova ◽  
A.A. Smirnova ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Zona Pellucida ◽  
Polar Body ◽  
New Technique ◽  
Laser Tweezers ◽  
Polar Body Biopsy ◽  
A New Technique

Material Behavior of 2D Steel Lattices with Different Unit-Cell Patterns

2021 ◽  
Vol 1046 ◽  
pp. 15-21
Author(s):  
Paiboon Limpitipanich ◽  
Pana Suttakul ◽  
Yuttana Mona ◽  
Thongchai Fongsamootr
Keyword(s):  
Elastic Modulus ◽  
Experimental Studies ◽  
Base Material ◽  
Unit Cell ◽  
Material Behavior ◽  
Closed Form Solutions ◽  
The Past ◽  
Weight Density ◽  
Unit Cells ◽  
Cell Patterns

Over the past years, two-dimensional lattices have attracted the attention of several researchers because they are lightweight compared with their full-solid counterparts, which can be used in various engineering applications. Nevertheless, since lattices are manufactured by reducing the base material, their stiffnesses then become lower. This study presents the weight efficiency of the lattices defined by relations between the elastic modulus and the weight density of the lattices. In this study, the mechanical behavior of 2D lattices is described by the in-plane elastic modulus. Experimental studies on the elastic modulus of the 2D lattices made of steel are performed. Three lattices having different unit cells, including square, body-centered, and triangular unit cells, are considered. The elastic modulus of each lattice is investigated by tensile testing. All specimens of the lattices are made of steel and manufactured by waterjet cutting. The experimental results of the elastic modulus of the lattices with the considered unit-cell patterns are validated with those obtained from finite element simulations. The results obtained in this study are also compared with the closed-form solutions founded in the literature. Moreover, the unit-cell pattern yielding the best elastic modulus for the lattice is discussed through weight efficiency.

scholarly journals A single-cell spiking model for the origin of grid-cell patterns

2017 ◽  
Vol 13 (10) ◽  
pp. e1005782 ◽  
Author(s):  
Tiziano D’Albis ◽  
Richard Kempter
Keyword(s):  
Single Cell ◽  
Grid Cell ◽  
Cell Patterns ◽  
Spiking Model

scholarly journals Evaluation of Escherichia coli Cell Response to Antibiotic Treatment by Use of Raman Spectroscopy with Laser Tweezers

2010 ◽  
Vol 48 (11) ◽  
pp. 4287-4290 ◽  
Author(s):  
T. J. Moritz ◽  
C. R. Polage ◽  
D. S. Taylor ◽  
D. M. Krol ◽  
S. M. Lane ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Raman Spectroscopy ◽  
Antibiotic Treatment ◽  
Cell Response ◽  
Coli Cell ◽  
Laser Tweezers
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