Disorder strength calculation for label-free diagnosis of tissue biopsies using quantitative phase imaging

2018 ◽  
Author(s):  
Masanori Takabayashi ◽  
Hassaan Majeed ◽  
Andre Kajdacsy-Balla ◽  
Gabriel Popescu
Keyword(s):  
Strength Calculation ◽  
Phase Imaging ◽  
Label Free ◽  
Disorder Strength ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase

scholarly journals Reagent- and actuator-free analysis of individual erythrocytes using three-dimensional quantitative phase imaging and capillary microfluidics

2021 ◽  
Author(s):  
DongHun Ryu ◽  
Hyeono Nam ◽  
Jessie Sungyun Jeon ◽  
YongKeun Park
Keyword(s):  
Blood Cells ◽  
Histopathological Examination ◽  
Point Of Care ◽  
Hemoglobin Concentration ◽  
Biochemical Properties ◽  
Phase Imaging ◽  
Sample Treatment ◽  
Label Free ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase

Histopathological examination of blood cells plays a crucial role in the diagnosis of various diseases. However, it involves time-consuming and laborious staining procedures required for microscopic review by medical experts and is not directly applicable for point-of-care diagnosis in resource-limited locations. This study reports a dilution-, actuation- and label-free method for the analysis of individual red blood cells (RBCs) using a capillary microfluidic device and quantitative phase imaging. Blood, without any sample treatment, is directly loaded into a micrometer-thick channel such that it forms a quasi-monolayer inside the channel. The morphological and biochemical properties of RBCs, including hemoglobin concentration, hemoglobin content, and corpuscular volume, were retrieved using the refractive index tomograms of individual RBCs measured using 3D quantitative phase imaging. The deformability of individual RBCs was also obtained by measuring the dynamic membrane fluctuations. The proposed framework applies to other imaging modalities and biomedical applications, facilitating rapid and cost-effective diagnosis and prognosis of diseases.

scholarly journals Tissue disorder strength measured by quantitative phase imaging as intrinsic cancer marker

2017 ◽  
Author(s):  
Masanori Takabayashi ◽  
Hassaan Majeed ◽  
Andre Kajdacsy-Balla ◽  
Gabriel Popescu
Keyword(s):  
Refractive Index ◽  
Imaging System ◽  
Tissue Sample ◽  
Optical Path Length ◽  
Phase Imaging ◽  
Disorder Strength ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase ◽  
Path Length Difference ◽  
Malignant Breast

AbstractTissue refractive index provides important information about morphology at the nanoscale. Since the malignant transformation involves both intra- and inter-cellular changes in the refractive index map, the tissue disorder measurement can be used to extract important diagnosis information. Quantitative phase imaging (QPI) provides a practical means of extracting this information as it maps the optical path-length difference (OPD) across a tissue sample with sub-wavelength sensitivity. In this work, we employ QPI to compare the tissue disorder strength between benign and malignant breast tissue histology samples. Our results show that disease progression is marked by a significant increase in the disorder strength. Since our imaging system can be added as an upgrading module to an existing microscope, we anticipate that it can be integrated easily in the pathology work flow.

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