3D printed phantoms of retinal photoreceptor cells for evaluating adaptive optics imaging modalities

Author(s):  
Anant Agrawal ◽  
Nikita Kedia ◽  
Zhuolin Liu ◽  
Ryan Sochol ◽  
Daniel X. Hammer
Author(s):  
W. Krebs ◽  
I. Krebs

Various inclusion bodies occur in vertebrate retinal photoreceptor cells. Most of them are membrane bound and associated with phagocytosis or they are age related residual bodies. We found an additional inclusion body in foveal cone cells of the baboon (Papio anubis) retina.The eyes of a 15 year old baboon were fixed by immersion in cacodylate buffered glutaraldehyde (2%)/formaldehyde (2%) as described in detail elsewhere . Pieces of retina from various locations, including the fovea, were embedded in epoxy resin such that radial or tangential sections could be cut.Spindle shaped inclusion bodies were found in the cytoplasm of only foveal cones. They were abundant in the inner segments, close to the external limiting membrane (Fig. 1). But they also occurred in the outer fibers, the perikarya, and the inner fibers (Henle’s fibers) of the cone cells. The bodies were between 0.5 and 2 μm long. Their central diameter was 0.2 to 0. 3 μm. They always were oriented parallel to the long axis of the cone cells. In longitudinal sections (Figs. 2,3) they seemed to have a fibrous skeleton that, in cross sections, turned out to consist of plate-like (Fig.4) and tubular profiles (Fig. 5).


2015 ◽  
Vol 579 ◽  
pp. A1 ◽  
Author(s):  
A. La Camera ◽  
L. Schreiber ◽  
E. Diolaiti ◽  
P. Boccacci ◽  
M. Bertero ◽  
...  

2017 ◽  
Vol 472 (1) ◽  
pp. 217-223 ◽  
Author(s):  
Mischa Schirmer ◽  
Vincent Garrel ◽  
Gaetano Sivo ◽  
Eduardo Marin ◽  
Eleazar R. Carrasco

2017 ◽  
Vol 54 (3) ◽  
pp. 621-639 ◽  
Author(s):  
Weston A. Marlow ◽  
Ashley K. Carlton ◽  
Hyosang Yoon ◽  
James R. Clark ◽  
Christian A. Haughwout ◽  
...  

2006 ◽  
Vol 2 (S234) ◽  
pp. 71 ◽  
Author(s):  
C. Sánchez Contreras ◽  
D. Le Mignant ◽  
R. Sahai ◽  
F. H. Chaffee ◽  
M. Morris

2019 ◽  
Vol 623 ◽  
pp. A6 ◽  
Author(s):  
R. JL. Fétick ◽  
L. Jorda ◽  
P. Vernazza ◽  
M. Marsset ◽  
A. Drouard ◽  
...  

Context. Over the past decades, several interplanetary missions have studied small bodies in situ, leading to major advances in our understanding of their geological and geophysical properties. These missions, however, have had a limited number of targets. Among them, the NASA Dawn mission has characterised in detail the topography and albedo variegation across the surface of asteroid (4) Vesta down to a spatial resolution of ~20 m pixel−1 scale. Aims. Here our aim was to determine how much topographic and albedo information can be retrieved from the ground with VLT/SPHERE in the case of Vesta, having a former space mission (Dawn) providing us with the ground truth that can be used as a benchmark. Methods. We observed Vesta with VLT/SPHERE/ZIMPOL as part of our ESO large programme (ID 199.C-0074) at six different epochs, and deconvolved the collected images with a parametric point spread function (PSF). We then compared our images with synthetic views of Vesta generated from the 3D shape model of the Dawn mission, on which we projected Vesta’s albedo information. Results. We show that the deconvolution of the VLT/SPHERE images with a parametric PSF allows the retrieval of the main topographic and albedo features present across the surface of Vesta down to a spatial resolution of ~20–30 km. Contour extraction shows an accuracy of ~1 pixel (3.6 mas). The present study provides the very first quantitative estimate of the accuracy of ground-based adaptive-optics imaging observations of asteroid surfaces. Conclusions. In the case of Vesta, the upcoming generation of 30–40 m telescopes (ELT, TMT, GMT) should in principle be able to resolve all of the main features present across its surface, including the troughs and the north–south crater dichotomy, provided that they operate at the diffraction limit.


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