Multimodal nanoscale imaging of chromatin with super resolution microscopy and partial wave spectroscopy (Conference Presentation)

2018 ◽  
Author(s):  
Adam Eshein ◽  
Xiang Zhou ◽  
Luay M. Almassalha ◽  
Scott Gladstein ◽  
Yue Li ◽  
...  
Keyword(s):  
Partial Wave ◽  
Super Resolution ◽  
Nanoscale Imaging ◽  
Super Resolution Microscopy ◽  
Partial Wave Spectroscopy

scholarly journals Enhanced super-resolution microscopy by extreme value based emitter recovery

2018 ◽  
Author(s):  
Hongqiang Ma ◽  
Wei Jiang ◽  
Jianquan Xu ◽  
Yang Liu
Keyword(s):  
Super Resolution ◽  
Extreme Value ◽  
New Approach ◽  
Manual Adjustment ◽  
Imaging Characteristics ◽  
Nanoscale Imaging ◽  
Super Resolution Microscopy ◽  
Adjustment Of Parameters ◽  
Imagej Plugin

ABSTRACTSuper-resolution localization microscopy allows visualization of biological structure at nanoscale resolution. However, the presence of heterogeneous background can degrade the nanoscale resolution by tens of nanometers and introduce significant image artifacts. Here we develop a new approach, referred to as extreme value based emitter recovery (EVER), to accurately recover the distorted fluorescent emitters from heterogeneous background. Through numerical simulation and biological experiments, we demonstrate that EVER significantly improves the accuracy and fidelity of the reconstructed super-resolution image for a wide variety of imaging characteristics. EVER requires no manual adjustment of parameters and is implemented as an easy-to-use ImageJ plugin that can immediately enhance the quality of super-resolution images. Our method paves the way for accurate nanoscale imaging of samples with heterogeneous background fluorescence, such as thicker tissue and cells.

scholarly journals Corrected Super-Resolution Microscopy Enables Nanoscale Imaging of Autofluorescent Lung Macrophages

2020 ◽  
Vol 119 (12) ◽  
pp. 2403-2417
Author(s):  
Ashley R. Ambrose ◽  
Susanne Dechantsreiter ◽  
Rajesh Shah ◽  
M. Angeles Montero ◽  
Anne Marie Quinn ◽  
...  
Keyword(s):  
Super Resolution ◽  
Lung Macrophages ◽  
Nanoscale Imaging ◽  
Super Resolution Microscopy

scholarly journals Enhanced super-resolution microscopy by extreme value based emitter recovery

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hongqiang Ma ◽  
Wei Jiang ◽  
Jianquan Xu ◽  
Yang Liu
Keyword(s):  
Super Resolution ◽  
Extreme Value ◽  
Biological Structure ◽  
Manual Adjustment ◽  
Imaging Characteristics ◽  
Nanoscale Imaging ◽  
Super Resolution Microscopy ◽  
Adjustment Of Parameters ◽  
Imagej Plugin

AbstractSuper-resolution localization microscopy allows visualization of biological structure at nanoscale resolution. However, the presence of heterogeneous background can degrade the nanoscale resolution by tens of nanometers and introduce significant image artifacts. Here we investigate and validate an efficient approach, referred to as extreme value-based emitter recovery (EVER), to accurately recover the distorted fluorescent emitters from heterogeneous background. Through numerical simulation and biological experiments, we validated the accuracy of EVER in improving the fidelity of the reconstructed super-resolution image for a wide variety of imaging characteristics. EVER requires no manual adjustment of parameters and has been implemented as an easy-to-use ImageJ plugin that can immediately enhance the quality of reconstructed super-resolution images. This method is validated as an efficient way for robust nanoscale imaging of samples with heterogeneous background fluorescence, such as thicker tissue and cells.

Super-Resolution Microscopy in Studying the Structure and Function of the Cell Nucleus

Acta Naturae ◽  
2017 ◽  
Vol 9 (4) ◽  
pp. 42-51
Author(s):  
S. S. Ryabichko ◽  
◽  
A. N. Ibragimov ◽  
L. A. Lebedeva ◽  
E. N. Kozlov ◽  
...  
Keyword(s):  
Cell Nucleus ◽  
Super Resolution ◽  
Structure And Function ◽  
And Function ◽  
Super Resolution Microscopy

Structural Evidence of Photoisomerization Pathways in Fluorescent Proteins

2019 ◽  
Author(s):  
Jeffrey Chang ◽  
Matthew Romei ◽  
Steven Boxer
Keyword(s):  
Rational Design ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Super Resolution ◽  
Unit Cell Size ◽  
Chlorine Substituent ◽  
Gfp Chromophore ◽  
The One ◽  
Green Fluorescent ◽  
Super Resolution Microscopy

<p>Double-bond photoisomerization in molecules such as the green fluorescent protein (GFP) chromophore can occur either via a volume-demanding one-bond-flip pathway or via a volume-conserving hula-twist pathway. Understanding the factors that determine the pathway of photoisomerization would inform the rational design of photoswitchable GFPs as improved tools for super-resolution microscopy. In this communication, we reveal the photoisomerization pathway of a photoswitchable GFP, rsEGFP2, by solving crystal structures of <i>cis</i> and <i>trans</i> rsEGFP2 containing a monochlorinated chromophore. The position of the chlorine substituent in the <i>trans</i> state breaks the symmetry of the phenolate ring of the chromophore and allows us to distinguish the two pathways. Surprisingly, we find that the pathway depends on the arrangement of protein monomers within the crystal lattice: in a looser packing, the one-bond-flip occurs, whereas in a tighter packing (7% smaller unit cell size), the hula-twist occurs.</p><p> </p><p> </p><p> </p><p> </p><p> </p><p> </p> <p> </p>

Novel organic dyes for multicolor localization-based super-resolution microscopy

2015 ◽  
Vol 9 (1-2) ◽  
pp. 161-170 ◽  
Author(s):  
Martin Lehmann ◽  
Gregor Lichtner ◽  
Haider Klenz ◽  
Jan Schmoranzer
Keyword(s):  
Organic Dyes ◽  
Super Resolution ◽  
Super Resolution Microscopy
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