Characterization of bone collagen organization defects in murine hypophosphatasia using a Zernike model of optical aberrations

2018 ◽  
Author(s):  
Kayvan F. Tehrani ◽  
Emily G. Pendleton ◽  
Ruth P. Barrow ◽  
Luke J. Mortensen ◽  
Bobby Leitmann
Keyword(s):  
Bone Collagen ◽  
Optical Aberrations ◽  
Collagen Organization

Generation and characterization of adjustable pure third-order spatial phase by tuning optical aberrations

2019 ◽  
Vol 21 (8) ◽  
pp. 085602 ◽  
Author(s):  
Daryoush Abdollahpour ◽  
Morteza Lotfollahi ◽  
Mohammad Yeganeh ◽  
Saifollah Rasouli
Keyword(s):  
Optical Aberrations ◽  
Third Order ◽  
Spatial Phase

scholarly journals FORMULASI SEDIAAN LOTION MENGGUNAKAN KOLAGEN TULANG IKAN PATIN (PANGASIUS SP) SEBAGAI PELEMBAB KULIT

2021 ◽  
Vol 4 (1) ◽  
pp. 401-408
Author(s):  
Hepni Hepni
Keyword(s):  
Statistical Test ◽  
Trial Result ◽  
Bone Collagen ◽  
Test Results ◽  
Infrared Spectrophotometry ◽  
Omega 3 ◽  
Free Trial ◽  
Oil In Water ◽  
Moisture Percentage

Background: In general, people consume catfish meat as a source of protein and a high content of omega 3, while the bones are disposed of as waste. Collagen is a protein derivative that has the effect of maintaining skin elasticity. Objective: To find out whether the catfish bone collagen can be formulated into lotions which in certain consentration can moisturize and do not irritate the skin. Method: Extraction of collagen from catfish bones, characterization of collagen by infrared spectrophotometry, making collagen lotion of catfish bones with a concentration of 1%, 1.5%, 2.5%, 3.5% and evaluation of collagen lotion preparations and test the ability of the preparation to moisturize. skin using a Skin moisturizer detector (SG- ed statistically using the ANOVA test with SPSS 20 Free trial. Result: Catfish bones contained collagen in waves of 3250-3350 cm-1 and 1260-1630 cm-1 which indicated the presence of amine and amide groups. The statistical test results show the probability is smaller than 0.05. The highest humidity was in the FE formula which contained 3.5% collagen with a moisture percentage of 53.0% with a level of "Moist" but lower than the lotions on the market with a percentage of 54.3%. Conclusion: Catfish bone collagen can be formulated into oil-in-water (M/A) emulsion type lotion.   Abstrak Pendahuluan: Secara umum masyarakat mengkonsumsi daging ikan patin sebagai sumber protein dan kandungan omega 3 yang tinggi, sedangkan tulangnya dibuang sebagai limbah. Kolagen merupakan salah satu turunan protein yang mempunyai efek untuk mempertahankan kekenyalan kulit. Tujuan: Untuk mengetahui apakah kolagen tulang ikan patin dapat diformulasikan kedalam sediaan lotion yang dalam konsentrasi tertentu dapat melembabkan dan tidak mengiritasi kulit. Metode: Ekstraksi kolagen dari tulang ikan patin, karakterisasi kolagen dengan spektrofotometri inframerah, pembuatan lotion kolagen tulang ikan patin dengan konsentrasi 1%, 1,5%, 2,5%, 3,5% dan evaluasi sediaan lotion kolagen serta uji kemampuan sediaan melembabkan kulit memakai alat Skin moisturizer detector (SG-7D ) serta hasilnya diuji secara statistik memakai uji ANOVA dengan SPSS 20 Free trial. Hasil: Tulang ikan patin mengandung kolagen pada gelombang 32503350 cm-1 dan 1260-1630 cm-1 yang menunjukkan adanya gugus amina dan amida. Hasil uji statistik menunjukkan probabilitas lebih kecil dari 0,05. Kelembaban tertinggi yaitu pada formula FE yang mengandung 3,5% kolagen dengan persentase kelembaban 53,0% dengan level ”Lembab” tetapi lebih rendah dari lotion yang beredar dipasaran dengan persentase sebesar 54,3%. Kesimpulan: Kolagen tulang ikan patin dapat diformulasi menjadi sediaan lotion tipe emulsi minyak dalam air (M/A).

Characterization of the optical aberrations of a metrological UV microscope

2021 ◽  
Author(s):  
Jan Krüger ◽  
Bernd Bodermann ◽  
Rainer Köning ◽  
Wolfgang Haessler-Grohne ◽  
Han Xu
Keyword(s):  
Optical Aberrations

Imaging structural and mechanical properties of articular cartilage using optical polarization tractography

2019 ◽  
Author(s):  
◽  
Mohammadreza Ravanfar
Keyword(s):  
Mechanical Properties ◽  
Articular Cartilage ◽  
Critical Role ◽  
Shear Loading ◽  
Optical Polarization ◽  
Optical Birefringence ◽  
Mechanical Responses ◽  
Collagen Organization ◽  
Radial Zone

[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Osteoarthritis (OA) is an extremely common joint disease, which affects more than one-third of all adults in the USA. Although the entire joint compartments are involved, OA is considered as a cartilage disease. Articular cartilage is a thin tissue covering the end of bones in the diarthrodial joints and plays a crucial role in providing a frictionless articulation. In spite of the harsh mechanical environment, cartilage has an amazingly long life due to its unique structure and composition. Cartilage is composed of ~80% water and ~20% solid matrix that mainly consists of collagen fibers and proteoglycans. Collagen degeneration is often an early symptom in OA. Since the fiber structure governs normal functionality in cartilage, the disease progression leads to impaired mechanical functions. Hence, an effective imaging technology that can visualize the collagen organization and its effects on cartilage mechanical properties will help to understand the sophisticated structure-function relationship in cartilage. Polarized light macroscopy (PLM) has been broadly utilized for collagen assessment; however, it requires thin, sectioned samples and thus remains a destructive technology. We introduced a nondestructive alternative to PLM for cartilage imaging using optical polarization tractography (OPT). OPT improved visualization and characterization of the zonal structure in cartilage by calculating the depth-resolved local birefringence and fiber orientation. We demonstrated that parametric imaging can be implemented using multiple complementary tissue contrasts obtained in OPT including surface roughness, birefringence, and fiber dispersion. We showed that parametric OPT imaging provided a morphometric evaluation of collagen damage in human OA cartilage samples. Because OPT can accurately quantify tissue optical birefringence, it can reveal the higher level of complexity in collagen architecture of cartilage. Our multi-incident OPT based biaxial birefringence measurement provided strong evidence of the existence of a leaf-like structure in cartilage. Furthermore, we expanded the capability of OPT technology by developing a method that can simultaneously image the fiber organization and mechanical responses in cartilage. This new method enabled a precise characterization of the zonal structural and mechanical responses to unconfined compressive and directional shear loading. We discovered that the upper part of the radial zone plays a critical role in absorbing compression-induced deformation in cartilage. Young's modulus in cartilage was strongly correlated with the optical birefringence. In the shear test, we found a remarkably higher shear modulus in the radial zone when the sample was sheared along the fibers. In summary, this dissertation research developed new OPT based imaging methods that can fully characterize the collagen organization and its responses during mechanical loading. This new technology has a great potential for nondestructive structural and functional imaging in articular cartilage.

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