3D Fluorescence Imaging cryomicrotome system for multispectral structural, functional and molecular imaging of whole organs (Conference Presentation)

2018 ◽  
Author(s):  
Paul R. Bloemen ◽  
Iwan J. Dobbe ◽  
Coen D. Dijkman ◽  
Niels H. Kind ◽  
Maurice C. G. Aalders ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Fluorescence Imaging

scholarly journals Multimodal Contrast Agents for Optoacoustic Brain Imaging in Small Animals

2021 ◽  
Vol 9 ◽  
Author(s):  
Xue-feng Shi ◽  
Bin Ji ◽  
Yanyan Kong ◽  
Yihui Guan ◽  
Ruiqing Ni
Keyword(s):  
Molecular Imaging ◽  
Contrast Agents ◽  
Brain Imaging ◽  
Fluorescence Imaging ◽  
Multimodal Imaging ◽  
Complex Disease ◽  
Rapid Development ◽  
Brain Diseases ◽  
Small Animals ◽  
Optoacoustic Imaging

Optoacoustic (photoacoustic) imaging has demonstrated versatile applications in biomedical research, visualizing the disease pathophysiology and monitoring the treatment effect in an animal model, as well as toward applications in the clinical setting. Given the complex disease mechanism, multimodal imaging provides important etiological insights with different molecular, structural, and functional readouts in vivo. Various multimodal optoacoustic molecular imaging approaches have been applied in preclinical brain imaging studies, including optoacoustic/fluorescence imaging, optoacoustic imaging/magnetic resonance imaging (MRI), optoacoustic imaging/MRI/Raman, optoacoustic imaging/positron emission tomography, and optoacoustic/computed tomography. There is a rapid development in molecular imaging contrast agents employing a multimodal imaging strategy for pathological targets involved in brain diseases. Many chemical dyes for optoacoustic imaging have fluorescence properties and have been applied in hybrid optoacoustic/fluorescence imaging. Nanoparticles are widely used as hybrid contrast agents for their capability to incorporate different imaging components, tunable spectrum, and photostability. In this review, we summarize contrast agents including chemical dyes and nanoparticles applied in multimodal optoacoustic brain imaging integrated with other modalities in small animals, and provide outlook for further research.

scholarly journals Molecular imaging needles: dual-modality optical coherence tomography and fluorescence imaging of labeled antibodies deep in tissue

2015 ◽  
Vol 6 (5) ◽  
pp. 1767 ◽  
Author(s):  
Loretta Scolaro ◽  
Dirk Lorenser ◽  
Wendy-Julie Madore ◽  
Rodney W. Kirk ◽  
Anne S. Kramer ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Molecular Imaging ◽  
Fluorescence Imaging ◽  
Optical Coherence ◽  
Dual Modality

scholarly journals Molecular Design of d-Luciferin-Based Bioluminescence and 1,2-Dioxetane-Based Chemiluminescence Substrates for Altered Output Wavelength and Detecting Various Molecules

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1618
Author(s):  
Hideo Takakura
Keyword(s):  
Molecular Imaging ◽  
Fluorescence Imaging ◽  
In Vivo Imaging ◽  
Molecular Design ◽  
Design Strategies ◽  
Deep Tissue ◽  
Excitation Light ◽  
Popular Method ◽  
Output Wavelength

Optical imaging including fluorescence and luminescence is the most popular method for the in vivo imaging in mice. Luminescence imaging is considered to be superior to fluorescence imaging due to the lack of both autofluorescence and the scattering of excitation light. To date, various luciferin analogs and bioluminescence probes have been developed for deep tissue and molecular imaging. Recently, chemiluminescence probes have been developed based on a 1,2-dioxetane scaffold. In this review, the accumulated findings of numerous studies and the design strategies of bioluminescence and chemiluminescence imaging reagents are summarized.

The influence of confocal microscope design on imaging performance

1993 ◽  
Vol 51 ◽  
pp. 144-145
Author(s):  
C J R Sheppard
Keyword(s):  
Image Quality ◽  
Fluorescence Imaging ◽  
Confocal Microscope ◽  
Industrial Applications ◽  
Three Dimensions ◽  
Design Parameters ◽  
Weak Signals ◽  
Size And Shape ◽  
Imaging Performance ◽  
Fundamental Limitation

The confocal microscope is now widely used in both biomedical and industrial applications for imaging, in three dimensions, objects with appreciable depth. There are now a range of different microscopes on the market, which have adopted a variety of different designs. The aim of this paper is to explore the effects on imaging performance of design parameters including the method of scanning, the type of detector, and the size and shape of the confocal aperture.It is becoming apparent that there is no such thing as an ideal confocal microscope: all systems have limitations and the best compromise depends on what the microscope is used for and how it is used. The most important compromise at present is between image quality and speed of scanning, which is particularly apparent when imaging with very weak signals. If great speed is not of importance, then the fundamental limitation for fluorescence imaging is the detection of sufficient numbers of photons before the fluorochrome bleaches.

Ubiquinone-BODIPY nanoparticles for tumor redox-responsive fluorescence imaging and photodynamic activity

2021 ◽  
Author(s):  
Byunghee Hwang ◽  
Tae-Il Kim ◽  
Hyunjin Kim ◽  
Sungjin Jeon ◽  
Yongdoo Choi ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Selective Activation ◽  
Turn On ◽  
Intracellular Environment ◽  
Redox Responsive ◽  
Photodynamic Activity

A ubiquinone-BODIPY photosensitizer self-assembles into nanoparticles (PS-Q-NPs) and undergoes selective activation within the highly reductive intracellular environment of tumors, resulting in “turn-on” fluorescence and photosensitizing activities.

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